The Multifaceted Effects of Polysaccharides Isolated from Dendrobium huoshanense on Immune Functions with the Induction of Interleukin-1 Receptor Antagonist (IL-1ra) in Monocytes

Abstract

Dendrobium huoshanense is a valuable and versatile Chinese herbal medicine with the anecdotal claims of cancer prevention and anti-inflammation. However, its immunological activities are limited to in vitro studies on a few cytokines and immune cell functions. First, we investigated the effects of polysaccharides isolated from DH (DH-PS) on inducing a panel of cytokines/chemokines in mice in vivo and human in vitro. We found that DH polysaccharides (DH-PS) induced TH1, TH2, inflammatory cytokines and chemokines in mouse in vivo and human cells in vitro. Secondly, we demonstrated that DH-PS expanded mouse splenocytes in vivo including CD4+ T cells, CD8+ T cells, B cells, NK cells, NKT cells, monocytes/macrophages, granulocytes and regulatory T cells. Notably, DH-PS induced an anti-inflammatory molecule, IL-1ra, in mouse and human immune cells, especially monocytes. The serum level of IL-1ra elicited by the injection of DH-PS was over 10 folds of IL-1β, suggesting that DH-PS-induced anti-inflammatory activities might over-ride the inflammatory ones mediated by IL-1β. The signaling pathways of DH-PS-induced IL-1ra production was shown to involve ERK/ELK, p38 MAPK, PI3K and NFκB. Finally, we observed that IL-1ra level induced by DH-PS was significantly higher than that by F3, a polysaccharide extract isolated from another popular Chinese herbal medicine, Ganoderma lucidum. These results indicated that DH-PS might have potential applications for ameliorating IL-1-induced pathogenic conditions.

Evaluation of Chemical Constituents and Important Mechanism of Pharmacological Biology inDendrobiumPlants

“…Interestingly, the total polysaccharides of D. huoshanense induced the expression of interleukin-1 receptor antagonist (IL-1ra) in human monocytes. Which the IL-1ra was regulated by 37 a series of signaling pathways, like ERK/ELK, p38 MAPK, PI3K, and NF- κ B [ 83 ].…”

Dendrobium: Sources of Active Ingredients to Treat Age-Related Pathologies

“…DHP-4A consists of glucose, arabinose, mannose and rhamnose with a molar ratio of 13.8:3.0:6.1:2.1 [ 91 ]. Lin et al (2014) showed that D. huoshanense polysaccharides (DH-PS) induced a Th1, Th2, inflammatory cytokines and chemokines in mice in vivo and human in vitro [ 92 ]. DH-PS expanded mouse splenocytes in vivo including CD4 + T cells, CD8 + T cells, B cells, NK cells, NKT cells, monocytes/macrophages, granulocytes and regulatory T cells.…”

First Systematic Analysis for Chemical Composition by HPLC-ESI-MSn and Antioxidant Activity Comparison of Dendrobium huoshanense and Dendrobium officinale

“…Now, D. huoshanense is known as national first-level protected plant and Chinese National Geographic Indication product, and it was officially included in the 2020 edition of the Chinese Pharmacopoeia. D. huoshanense has a variety of pharmacological effects such as anti-oxidation activity, liver protection, immunomodulatory activity, anti-cataract activity, promotion of saliva production and building of immunity [8][9][10][11]. Due to their unique habitat, the slow growth rate and over-harvesting, D. huoshanense is on the verge of extinction and expensive.…”