Enhancement of bone healing using non‐glycosylated rhBMP‐2 released from a fibrin matrix in dogs and cats

Schmoekel, Hugo G.; Weber, Franz E.; Hurter, Karin; Schense, Jason C.; Seiler, Gabriela; Rytz, Ulrich; Spreng, David; Schawalder, Peter; Hubbell, Jeffrey A.

doi:10.1111/j.1748-5827.2005.tb00269.x

Cited by 22 publications

(37 citation statements)

References 23 publications

(7 reference statements)

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“…Consequently ECM bound VEGF has become an attractive tool to induce vascularization of bioengineered tissue substitutes [135]. In parallel other growth factors or peptides with bioactive potency have been employed as ECM bound mediators of angiogenesis, cell proliferation and differentiation [136,137]. Other concepts of biologically interactive ECM materials were soon to follow.…”

Section: Smart Biomaterialsmentioning

confidence: 98%

Myocardial tissue engineering: the extracellular matrix☆

Akhyari¹,

Kamiya²,

Haverich³

et al. 2008

European Journal of Cardio-Thoracic Surgery

115

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More than a decade after the first reports on successful three-dimensional cardiac cell culture for experimental and potential therapeutic application, the interest and experimental efforts in the field of myocardial tissue engineering continues to grow. The hope that tissue cultures may one day act as graft substitute for malfunctioning myocardium continues to drive current scientific activity. Against this background interest seem to have progressively shifted towards the aim of engineering single tissue components. Accordingly, elements of the extracellular matrix (ECM) have gained increasing attention as potentially crucial mediators in developing and maintaining the characteristics of three-dimensional cardiac cell cultures. The ECM is now no longer regarded as merely a scaffold for developing tissue, a concept that is widely acknowledged in modern tissue engineering. The understanding of the role of precursor and stem cells has highlighted new complicated aspects of cell proliferation and differentiation and ECM proves to play an important role in providing essential signals to influence major intracellular pathways such as proliferation, differentiation and cell metabolism. Furthermore, progress in biochemical engineering has provided the perspective of application of synthetic ECM-linked molecules with bioactive potential. With the advent and continuous refinement of cell removal techniques, a new class of native acellular ECM has emerged with some striking advantages. The presently available ECM materials aim to closely resemble the in vivo microenvironment by acting as an active component of the developing tissue construct. It is therefore not surprising that most of the focus in myocardial tissue engineering has been on cell-matrix interaction, for both naturally derived and synthetic ECM. This article provides a review of established models of myocardial tissue engineering with respect to the employed ECM materials including current frontiers in material development.

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Section: Smart Biomaterialsmentioning

confidence: 98%

Myocardial tissue engineering: the extracellular matrix☆

Akhyari¹,

Kamiya²,

Haverich³

et al. 2008

European Journal of Cardio-Thoracic Surgery

115

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show abstract

“…158 The utility of ng1BMP-2 was also confirmed in a series of prospective veterinary clinical trials. 158,161 The same group in another strategy designed a tripartite fusion protein, referred to as TG-pl-BMP-2 with TG denoting a transglutaminase-sensitive binding domain and pl the plasmin-sensitive linking domain. TG-pl-BMP-2 can be covalently incorporated into a fibrin matrix under the influence of the blood transglutaminase factor XIIIa, while a plasmin substrate provides a cleavage site for the local release of the attached growth factor under the influence of cell-activated plasmin.…”

mentioning

confidence: 99%

A review of fibrin and fibrin composites for bone tissue engineering

Noori

Ashrafi

Vaez-Ghaemi

et al. 2017

IJN

367

256

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Tissue engineering has emerged as a new treatment approach for bone repair and regeneration seeking to address limitations associated with current therapies, such as autologous bone grafting. While many bone tissue engineering approaches have traditionally focused on synthetic materials (such as polymers or hydrogels), there has been a lot of excitement surrounding the use of natural materials due to their biologically inspired properties. Fibrin is a natural scaffold formed following tissue injury that initiates hemostasis and provides the initial matrix useful for cell adhesion, migration, proliferation, and differentiation. Fibrin has captured the interest of bone tissue engineers due to its excellent biocompatibility, controllable biodegradability, and ability to deliver cells and biomolecules. Fibrin is particularly appealing because its precursors, fibrinogen, and thrombin, which can be derived from the patient’s own blood, enable the fabrication of completely autologous scaffolds. In this article, we highlight the unique properties of fibrin as a scaffolding material to treat bone defects. Moreover, we emphasize its role in bone tissue engineering nanocomposites where approaches further emulate the natural nanostructured features of bone when using fibrin and other nanomaterials. We also review the preparation methods of fibrin glue and then discuss a wide range of fibrin applications in bone tissue engineering. These include the delivery of cells and/or biomolecules to a defect site, distributing cells, and/or growth factors throughout other pre-formed scaffolds and enhancing the physical as well as biological properties of other biomaterials. Thoughts on the future direction of fibrin research for bone tissue engineering are also presented. In the future, the development of fibrin precursors as recombinant proteins will solve problems associated with using multiple or single-donor fibrin glue, and the combination of nanomaterials that allow for the incorporation of biomolecules with fibrin will significantly improve the efficacy of fibrin for numerous bone tissue engineering applications.

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“…These studies demonstrated much higher bone formation in test sites where fibrin carrier was loaded with rhBMP as compared to control sites. Other research groups have also reported development of fibrin matrices for delivery of rhBMPs [120,121,122]. These matrices were used to treat bone defects in rat, cats and dogs.…”

Section: Carriers For Bmpsmentioning

confidence: 99%

Bone Regeneration Using Bone Morphogenetic Proteins and Various Biomaterial Carriers

et al. 2015

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Trauma and disease frequently result in fractures or critical sized bone defects and their management at times necessitates bone grafting. The process of bone healing or regeneration involves intricate network of molecules including bone morphogenetic proteins (BMPs). BMPs belong to a larger superfamily of proteins and are very promising and intensively studied for in the enhancement of bone healing. More than 20 types of BMPs have been identified but only a subset of BMPs can induce de novo bone formation. Many research groups have shown that BMPs can induce differentiation of mesenchymal stem cells and stem cells into osteogenic cells which are capable of producing bone. This review introduces BMPs and discusses current advances in preclinical and clinical application of utilizing various biomaterial carriers for local delivery of BMPs to enhance bone regeneration.

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Enhancement of bone healing using non‐glycosylated rhBMP‐2 released from a fibrin matrix in dogs and cats

Abstract: This prospective clinical study demonstrates the beneficial effects of ngly-rhBMP-2 in a specially designed fibrin matrix on the treatment of bone defects, and validates the use of this composite as an alternative to bone autografts in dogs and cats.

Cited by 22 publications

References 23 publications

Myocardial tissue engineering: the extracellular matrix☆

Myocardial tissue engineering: the extracellular matrix☆

A review of fibrin and fibrin composites for bone tissue engineering

Bone Regeneration Using Bone Morphogenetic Proteins and Various Biomaterial Carriers

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