Enhancement of bone formation in hydroxyapatite implants by rhBMP‐2 coating

Schnettler, Reinhard; Knöß, Peter D.; Heiß, Christian; Stahl, J.P.; Meyer, Cindy; Kilián, O.; Wenisch, Sabine; Alt, Volker

doi:10.1002/jbm.b.31255

Cited by 23 publications

(12 citation statements)

References 30 publications

(53 reference statements)

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“…The bone healing process is associated with the presence of osteoregenerative cells (osteoblastic cells), osteopromotive proteins (growth factors, like BMP‐2,4,6,7), and local environment (supplied by a material carrier) (Ripamonti, 2006). This study used rhBMP‐2, previously described in literature (Issa et al, 2008; Schnettler et al, 2009; Wang et al, 2009) as a protein presenting osteoinductive capability, and a protein extracted from Hevea brasiliensis (P‐1), which is currently being characterized. When the surgical area was covered with collagen, a higher amount of newly formed bone was found with the exception of Group IV with pure P‐1.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of rhBMP‐2 and Natural Latex as Potential Osteogenic Proteins in Critical Size Defects by Histomorphometric Methods

Issa

Defino

Netto

et al. 2010

The Anatomical Record

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This in vivo study evaluated the osteogenic potential of two proteins, recombinant human bone morphogenetic protein-2 (rhBMP-2) and a protein extracted from natural latex (Hevea brasiliensis, P-1), and compared their effects on bone defects when combined with a carrier or a collagen gelatin. Eighty-four (84) Wistar rats were divided into two groups, with and without the use of collagen gelatin, and each of these were divided into six treatment groups of seven animals each. The treatment groups were: (1) 5 lg of pure rhBMP-2; (2) 5 lg of rhBMP-2/monoolein gel; (3) pure monoolein gel; (4) 5 lg of pure P-1; (5) 5 lg of P-1/monoolein gel; (6) critical bone defect control. The animals were anesthetized and a 6 mm diameter critical bone defect was made in the left posterior region of the parietal bone. Animals were submitted to intracardiac perfusion after 4 weeks and the calvaria tissue was removed for histomorphometric analysis. In this experimental study, it was concluded that rhBMP-2 allowed greater new bone formation than P-1 protein and this process was more effective when the bone defect was covered with collagen gelatin (P < 0.05). Anat Rec,

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Section: Discussionmentioning

confidence: 99%

Evaluation of rhBMP‐2 and Natural Latex as Potential Osteogenic Proteins in Critical Size Defects by Histomorphometric Methods

Issa

Defino

Netto

et al. 2010

The Anatomical Record

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“…In contrast to healthy bone tissue which constantly adjusts its structure to varying environmental needs [6], these materials cannot be remodeled, often resulting in material fatigue, loosening and implant failure. Hence, an important characteristic of synthetic bone substitutes is their bioactivity, which should ideally allow a defined resorption that is balanced with the speed of new bone formation, resulting in true osseointegration and restitutio ad integrum [7], [8]. In this context it is important to state that so called “resorbable” bone substitutes, including calcium phosphates, calcium sulphates, and calcium carbonates, have been somewhat unsuccessful as they were still detectable years after implantation due to impaired resorption.…”

Section: Introductionmentioning

confidence: 99%

Divergent Resorbability and Effects on Osteoclast Formation of Commonly Used Bone Substitutes in a Human In Vitro-Assay

et al. 2012

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Bioactive bone substitute materials are a valuable alternative to autologous bone transplantations in the repair of skeletal defects. However, clinical studies have reported varying success rates for many commonly used biomaterials. While osteoblasts have traditionally been regarded as key players mediating osseointegration, increasing evidence suggests that bone-resorbing osteoclasts are of crucial importance for the longevity of applied biomaterials. As no standardized data on the resorbability of biomaterials exists, we applied an in vitro-assay to compare ten commonly used bone substitutes. Human peripheral blood mononuclear cells (PBMCs) were differentiated into osteoclasts in the co-presence of dentin chips and biomaterials or dentin alone (control) for a period of 28 days. Osteoclast maturation was monitored on day 0 and 14 by light microscopy, and material-dependent changes in extracellular pH were assessed twice weekly. Mature osteoclasts were quantified using TRAP stainings on day 28 and their resorptive activity was determined on dentin (toluidin blue staining) and biomaterials (scanning electron microscopy, SEM). The analyzed biomaterials caused specific changes in the pH, which were correlated with osteoclast multinuclearity (r = 0.942; p = 0.034) and activity on biomaterials (r = 0.594; p = 0.041). Perossal led to a significant reduction of pH, nuclei per osteoclast and dentin resorption, whereas Tutogen bovine and Tutobone human strikingly increased all three parameters. Furthermore, natural biomaterials were resorbed more rapidly than synthetic biomaterials leading to differential relative resorption coefficients, which indicate whether bone substitutes lead to a balanced resorption or preferential resorption of either the biomaterial or the surrounding bone. Taken together, this study for the first time compares the effects of widely used biomaterials on osteoclast formation and resorbability in an unbiased approach that may now aid in improving the preclinical evaluation of bone substitute materials.

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“…One of the strategies to enhance bone healing around implant, and therefore implant fixation and osseointegration, is to use the HA coating on the implant as a carrier for in situ delivering of drugs, such as bone morphogenetic proteins (BMP) 17. The incorporation of the drug during the process of the coating formation will ensure a sustained release of the drug into the surrounding bony tissue after the joint replacement surgery.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and characterization of acryloyloxy guar gum

Shenoy

D’Melo

2010

J of Applied Polymer Sci

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Guar gum is a galactomannan commonly used as a viscosity modifier in the food, pharmaceutical, and cosmetics industry. The aim of this study was to synthesize acryloyloxy guar gum via a Schotten-Bauman reaction in aqueous media. The reaction products were characterized using FTIR, C 13 -NMR, wide angle X-ray diffraction techniques to ascertain the effect of acrylation on the structure of guar gum. The acrylation of guar gum was found to be limited to the primary hydroxyl groups on the guar gum molecule. The maximum degree of substitution (DS) was found to be 0.56, which was observed after 3 h of reaction. Since the reaction was carried out in an aqueous medium after 3 h of reaction the DS of the derivatised guar gum was found to decrease, because of hydrolysis of the formed ester linkages. The ester content and intrinsic viscosity of the derivatised guar gums were also evaluated. Thermal analysis showed that a higher DS resulted in products with lower thermal stability and there was no evidence of reaction via the acrylate groups on heating.

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Enhancement of bone formation in hydroxyapatite implants by rhBMP‐2 coating

Cited by 23 publications

References 30 publications

Evaluation of rhBMP‐2 and Natural Latex as Potential Osteogenic Proteins in Critical Size Defects by Histomorphometric Methods

Evaluation of rhBMP‐2 and Natural Latex as Potential Osteogenic Proteins in Critical Size Defects by Histomorphometric Methods

Divergent Resorbability and Effects on Osteoclast Formation of Commonly Used Bone Substitutes in a Human In Vitro-Assay

Synthesis and characterization of acryloyloxy guar gum

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