Evaluation of rhBMP‐2 and Natural Latex as Potential Osteogenic Proteins in Critical Size Defects by Histomorphometric Methods

Abstract

This in vivo study evaluated the osteogenic potential of two proteins, recombinant human bone morphogenetic protein-2 (rhBMP-2) and a protein extracted from natural latex (Hevea brasiliensis, P-1), and compared their effects on bone defects when combined with a carrier or a collagen gelatin. Eighty-four (84) Wistar rats were divided into two groups, with and without the use of collagen gelatin, and each of these were divided into six treatment groups of seven animals each. The treatment groups were: (1) 5 lg of pure rhBMP-2; (2) 5 lg of rhBMP-2/monoolein gel; (3) pure monoolein gel; (4) 5 lg of pure P-1; (5) 5 lg of P-1/monoolein gel; (6) critical bone defect control. The animals were anesthetized and a 6 mm diameter critical bone defect was made in the left posterior region of the parietal bone. Animals were submitted to intracardiac perfusion after 4 weeks and the calvaria tissue was removed for histomorphometric analysis. In this experimental study, it was concluded that rhBMP-2 allowed greater new bone formation than P-1 protein and this process was more effective when the bone defect was covered with collagen gelatin (P < 0.05). Anat Rec,

Effects of the combination of low-level laser irradiation and recombinant human bone morphogenetic protein-2 in bone repair

“…The amounts of rhBMP-2, which aim to cover the area of the bone defect, are the subject of much discussion in the literature, and are dependent on several factors including the experimental model used, the size of the bone defect and time [30][31][32][33]. Different amounts of rhBMP-2 have been reported in the literature [7,9,34,35]. However, there is consensus that the use of a carrier material is necessary to improve delivery of the protein, considering that it is very expensive and expands quickly from the site where it is placed.…”

“…Several experimental models for bone repair evaluation using osteogenic materials have been described in the literature, using different areas, as critical bone defects in long bones [5], spinal cord [6], mandible [7,8] and calvaria [9], and involving surgical techniques such as distraction osteogenesis [10,11]. Thus, the aim of the present study was to evaluate histomorphometrically the action of LLLI and recombinant human BMP-2 (rhBMP-2) with or without a carrier on the process of healing of critical bone defects.…”

Estudo qualitativo e quantitativo da interação entre a proteína morfogenética rhBMP-2 e diferentes tipos de enxertos ósseos

“…cerâmicas, matriz óssea desmineralizada, compostos ricos em colágeno, fibrina, fosfato de cálcio, ácido hialurônico, proteína extraída da Hevea brasiliensis, polímeros derivados da mamona, assim como BMPs e enxertos ósseos (BEZERRA et al, 2012;BURG;PORTER;KELLAM, 2000;FIGUEIREDO et al, 2004;ISSA et al, 2010).…”

“…Além disso, outros fatores como técnica cirúrgica interferem no reparo ósseo e dificultam a definição do termo. O DOC não se cura sem intervenção e constitui um desafio para a reconstrução óssea (CHENARD et al, 2012;COOPER et al, 2010 proteínas têm utilizados diversos animais como coelhos, ratos e cães (ISSA et al, 2010;SCHMOEKEL et al, 2004;SMITH et al, 2008).…”

“…A atividade de osteoindução das rhBMPs foi avaliada em estudos in vivo (FUJIMURA et al, 1995;ISSA et al, 2010;KUSUMOTO et al, 1995). Devido à potente ação osteoindutora observada nestes estudos, torna-se evidente o potencial dessas proteínas para uma grande variedade de aplicações clínicas como cirurgias reconstrutivas, defeitos ósseos provocados por doenças, perdas ósseas devido a alterações fisiológicas, dentre outras situações que requerem o reparo ósseo (BOYNE, 1995;MARDEN et al, 1994).…”