Enhancement of antimicrobial activity of pump inhibitors associating drugs

Abstract

Introduction: with the continuous emergence of pathogenic resistance to conventional drugs through efflux pumps, increasing efforts are directed toward discovering efflux inhibitory molecules.
 Methodology: in this study three P-glycoprotein (P13CP, P22CP, P34CP) efflux-inhibitors (EIs), belonging to the series of phenoxymethylquinoxalines capable to restore/potentiate the antiproliferative activity of doxorubicin and vincristine against human tumor cell lines and different antibiotics against clinical isolates, were investigated on 10 clinical strains of Candida and 12 clinical and ATCC strains of Gram positive and Gram-negative bacteria.
 Results: MFC values of FLC were reduced in all Candida strains by the P22CP and P34CP inhibitors, and in 5/10 fungal strains by the P13CP inhibitor.
 Conclusion: novel antibiotics with new modes of action are urgently required to suppress the rise of MDR bacteria. An alternative approach would be to identify molecules that can interfere with the process of efflux.

<p>Clinical and Microbiological Characteristics of Invasive and Hypervirulent <em>Klebsiella pneumoniae</em> Infections in a Teaching Hospital in China</p>

“…Contrarily, new antibacterial drugs may be a good treatment option. 15 Some new products of natural origin against K. pneumoniae were reported to be useful in hospital settings. [16][17][18][19][20] According to the clinical characteristics of invasive and non-invasive HvKP, fifty percent (22/43) of patients with invasive HvKP infection were mainly treated with antibiotics combined with abscess drainage, and their prognosis was good.…”

Antibiotic-Resistant and Non-Resistant Bacteria Display Similar Susceptibility to Dielectric Barrier Discharge Plasma

“…Further studies are also needed to clarify the inactivation mechanism of the DBD plasma torch including detailed analysis of bactericidal factors generated by the plasma that induce damage to DNA and proteins as well as cell surface degradation including LPS, which is a component of the outer membrane of Gram-negative bacteria. Antibiotic resistant bacteria employ multiple mechanisms to confer resistance including antibiotic inactivation, target-site modification, and reduction of cytoplasmic antibiotic concentration [ 32 ]. The specific type of antibiotic resistance mechanism might be related to plasma susceptibility.…”

<p>Methicillin-Resistant <em>Staphylococcus aureus</em> (MRSA): One Health Perspective Approach to the Bacterium Epidemiology, Virulence Factors, Antibiotic-Resistance, and Zoonotic Impact</p>

“…108,109 Furthermore, an alternative approach would be to identify molecules that can interfere with the process of efflux. 110 Hospitals and veterinary authorities must apply general precautions to reduce the widespread of MRSA in community, general hygiene practice, infection control routine, disinfection of the environment are necessary. The implementation of the HACCP guidelines is necessary during the handling and processing of meat products, milk, and milk products to minify the contamination of human food with MRSA.…”