Enhanced white matter reorganization and activated brain glucose metabolism by enriched environment following ischemic stroke: Micro PET/CT and MRI study

Li, Man-Zhong; Zhao, Yuming; Zhan, Yuan; Yang, Le; Feng, Xuefeng; Lu, Yun; Lei, Jianfeng; Zhao, Ting; Wang, Lei; Zhao, Hui

doi:10.1016/j.neuropharm.2020.108202

Cited by 26 publications

(23 citation statements)

References 62 publications

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“…Nonetheless, the majority of the studies on this model agrees that short term ischemia leads to selective neuronal loss in the striatum of the affected hemisphere and that TIA causes functional de cits in human patients 66 7 . White matter injury, as characterized by demyelination and axonal breakdown, was also found after MCAO in rats, and this injury played a critical role in these rodents' poststroke disability 13 . Segmentation of lesion constitutes a prerequisite for quantitative analysis in stroke research, but this is a tedious and bias/error-prone task when done manually.…”

Section: Discussionmentioning

confidence: 96%

“…Since demyelination and axonal breakdown play a critical role in poststroke disability, the detailed characterization of the brain lesion at the bre level (white matter) is increasingly recognized as an important outcome to measure 11 . Thus, quantifying the volume of the brain white matter over grey matter, either by quanti cation of microstructural differences or integrity of myelinated bres is of major importance to assess poststroke recovery 12,13 .…”

Section: Introductionmentioning

confidence: 99%

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High resolution micro-CT imaging in mice stroke models: from 3D detailed infarct characterization to automatic area segmentation

Gomes

Pinto

Matula

et al. 2022

Preprint

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Characterization of brain infarct lesion in rodent models of stroke is crucial to assess the outcome of stroke therapies and to study the disease pathophysiology. However, so far it has been mostly performed by: 1) histological methods, a time-consuming process that lead to significant flaws; or 2) via MRI imaging, which is faster, yielding 3D information but at high costs. High-resolution micro-CT imaging became, in the last decade, a simple, fast, and cheaper solution, allowing 3D sample analysis. Here, we describe that high-resolution micro-CT, either using iodine/OsO4 as contrast agents, can be successfully applied for fine quantification and localization of lesion size and edema volume in preclinical stroke models. We successfully correlated this new approach with the standard histological method TTC. In transient MCAO mouse stroke model, we were able to identify/quantify large lesioned areas (segmented in core/penumbra), up to degenerated finer striatal myelinated fibers in a transient-ischemic-attack (TIA) mouse model, at different timepoints post-ischemia, through manual/automatic segmentation approaches (deep learning). Furthermore, whole brain 3D reconstructions allow brain atlas co-registration with specific affected brain areas. Hence, the presented methodology, through iodine/OsO4 micro-CT imaging, constitutes a valuable advance for precise and detailed assessment of stroke outcomes in preclinical animal studies.

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Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

High resolution micro-CT imaging in mice stroke models: from 3D detailed infarct characterization to automatic area segmentation

Gomes

Pinto

Matula

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Moreover, 18 F-FDG is a fluorine 18-labeled glucose analog that is transported into the cell, phosphorylated, and trapped within the cytoplasm. Changes in the rate of glucose metabolism probably correspond to changes in neurotransmitter function [31]. More recently, 18 F-FDG microPET scan, a functional neuroimaging technique, has become available for visualizing brain activity by quantifying glucose consumption in rats.…”

Section: Discussionmentioning

confidence: 99%

Neuroprotective Effect of Valproic Acid on Salicylate-Induced Tinnitus

Song

Cho

Vijayakumar

et al. 2021

IJMS

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High-dose salicylate induces temporary moderate hearing loss and the perception of a high-pitched tinnitus in humans and animals. Previous studies demonstrated that high doses of salicylate increase N-methyl-d-aspartate (NMDA) receptor levels, resulting in a rise in Ca2+ influx and induction of excitotoxicity. Glutamate excitotoxicity is associated with failure in the maintenance of calcium homeostasis, mitochondrial dysfunction, and production of reactive oxygen species (ROS). Valproic acid (VPA) is widely used for the management of bipolar disorder, epilepsy, and migraine headaches, and is known to regulate NMDA receptor activity. In this study, we examined the beneficial effects of VPA in a salicylate-induced tinnitus model in vitro and in vivo. Cells were pretreated with VPA followed by salicylate treatment. The expression levels of NMDA receptor subunit NR2B, phosphorylated cAMP response element-binding protein—an apoptosis marker, and intracellular levels of ROS were measured using several biochemical techniques. We observed increased expression of NR2B and its related genes TNFα and ARC, increased intracellular ROS levels, and induced expression of cleaved caspase-3. These salicylate-induced changes were attenuated in the neuronal cell line SH-SY5Y and rat cortical neurons after VPA pretreatment. Together, these results provide evidence of the beneficial effects of VPA in a salicylate-induced temporary hearing loss and tinnitus model.

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“…[36][37][38] The pathological biochemical reaction of an ischemic stroke begins with energy deprivation induced by a lack of oxygen and glucose supply to local brain tissue. 39,40 This is followed by stimulation of neuronal depolarization and glutamate release, causing calcium inward flow and elevated sodium ion content in the intracytoplasmic, and more glutamate release which leads to cellular excitotoxicity and cellular swelling, 41 ion channel dysfunction, and massive reactive oxygen and/or nitrogen species, ROS/RNS or RONS production. 42 These pathological and biochemical changes at the cellular and molecular levels further spread to neighboring cells, activating a series of enzymatic cascade reactions that eventually lead to the cell membrane and mitochondrial damage and production of RONS 43,44 ; the production of RONS can further damage mitochondria and DNA, eventually leading to cellular necrosis or apoptosis.…”

Section: Pathophys Iology Of Perioper Ative S Troke Smentioning

confidence: 99%

Potential nanotherapeutic strategies for perioperative stroke

Zhao

Duan

et al. 2022

CNS Neurosci Ther

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With the advent of "global aging," stroke has become the world's second-deadliest disease after coronary heart disease, accounting for 10%-15% of global deaths. [1][2][3] The perioperative period can be a high-risk period for stroke because of the pathophysiological state of the patient, which is based on the disease, anesthesia, functional changes in the coagulation system and pharmacological factors. [4][5][6] Recently, the number of patients with perioperative acute ischemic stroke (PAIS) has increased significantly. 7 Regrettably, clinical approval for the prevention and treatment of perioperative ischemic stroke has not yet been obtained. 8 Therefore, the development of more therapeutic strategies for perioperative stroke is urgently needed. 9,10 Given the similar pathophysiology of perioperative

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Enhanced white matter reorganization and activated brain glucose metabolism by enriched environment following ischemic stroke: Micro PET/CT and MRI study

Cited by 26 publications

References 62 publications

High resolution micro-CT imaging in mice stroke models: from 3D detailed infarct characterization to automatic area segmentation

High resolution micro-CT imaging in mice stroke models: from 3D detailed infarct characterization to automatic area segmentation

Neuroprotective Effect of Valproic Acid on Salicylate-Induced Tinnitus

Potential nanotherapeutic strategies for perioperative stroke

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