Enhanced repair of meniscal hoop structure injuries using an aligned electrospun nanofibrous scaffold combined with a mesenchymal stem cell-derived tissue engineered construct

Shimomura, Kazunori; Rothrauff, Benjamin B.; Hart, David A.; Hamamoto, Shuichi; Kobayashi, Masato; Yoshikawa, Hideki; Tuan, Rocky S.; Nakamura, Norimasa

doi:10.1016/j.biomaterials.2018.11.009

Cited by 59 publications

(59 citation statements)

References 47 publications

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“…An increasing number of studies are supporting the idea that cell-based strategies effectively improve meniscus repair and regeneration 32 33. However, it is still not clear which cell type is most effective for meniscus repair 34 35.…”

Section: Discussionmentioning

confidence: 99%

Single-cell RNA-seq analysis identifies meniscus progenitors and reveals the progression of meniscus degeneration

et al. 2019

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ObjectivesThe heterogeneity of meniscus cells and the mechanism of meniscus degeneration is not well understood. Here, single-cell RNA sequencing (scRNA-seq) was used to identify various meniscus cell subsets and investigate the mechanism of meniscus degeneration.MethodsscRNA-seq was used to identify cell subsets and their gene signatures in healthy human and degenerated meniscus cells to determine their differentiation relationships and characterise the diversity within specific cell types. Colony-forming, multi-differentiation assays and a mice meniscus injury model were used to identify meniscus progenitor cells. We investigated the role of degenerated meniscus progenitor (DegP) cell clusters during meniscus degeneration using computational analysis and experimental verification.ResultsWe identified seven clusters in healthy human meniscus, including five empirically defined populations and two novel populations. Pseudotime analysis showed endothelial cells and fibrochondrocyte progenitors (FCP) existed at the pseudospace trajectory start. Melanoma cell adhesion molecule ((MCAM)/CD146) was highly expressed in two clusters. CD146+ meniscus cells differentiated into osteoblasts and adipocytes and formed colonies. We identified changes in the proportions of degenerated meniscus cell clusters and found a cluster specific to degenerative meniscus with progenitor cell characteristics. The reconstruction of four progenitor cell clusters indicated that FCP differentiation into DegP was an aberrant process. Interleukin 1β stimulation in healthy human meniscus cells increased CD318+ cells, while TGFβ1 attenuated the increase in CD318+ cells in degenerated meniscus cells.ConclusionsThe identification of meniscus progenitor cells provided new insights into cell-based meniscus tissue engineering, demonstrating a novel mechanism of meniscus degeneration, which contributes to the development of a novel therapeutic strategy.

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Section: Discussionmentioning

confidence: 99%

Single-cell RNA-seq analysis identifies meniscus progenitors and reveals the progression of meniscus degeneration

et al. 2019

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“…Electrospun nanofibers have found extensive applications in the energy, environment, and biomedical field [1]. In biomedical applications, adjusting the surface properties of the nanofibers (e.g., fiber orientation and patterned structure) is frequently employed to achieve specific cell adhesion and growth on fiber surfaces [9,10]. Favorable cell adhesion is the key to tissue regeneration [11], but cell adhesion could lead to serious undesirable consequences under certain circumstances, such as tendon adhesion [12], intestinal adhesion [13], and intrauterine adhesion [14].…”

Section: Introductionmentioning

confidence: 99%

Hydration-Enhanced Lubricating Electrospun Nanofibrous Membranes Prevent Tissue Adhesion

et al. 2020

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Lubrication is the key to efficient function of human tissues and has significant impact on the comfort level. However, the construction of a lubricating nanofibrous membrane has not been reported as yet, especially using a one-step surface modification method. Here, bioinspired by the superlubrication mechanism of articular cartilage, we successfully construct hydration-enhanced lubricating nanofibers via one-step in situ grafting of a copolymer synthesized by dopamine methacrylamide (DMA) and 2-methacryloyloxyethyl phosphorylcholine (MPC) onto electrospun polycaprolactone (PCL) nanofibers. The zwitterionic MPC structure provides the nanofiber surface with hydration lubrication behavior. The coefficient of friction (COF) of the lubricating nanofibrous membrane decreases significantly and is approximately 65% less than that of pure PCL nanofibers, which are easily worn out under friction regardless of hydration. The lubricating nanofibers, however, show favorable wear-resistance performance. Besides, they possess a strong antiadhesion ability of fibroblasts compared with pure PCL nanofibers. The cell density decreases approximately 9-fold, and the cell area decreases approximately 12 times on day 7. Furthermore, the in vivo antitendon adhesion data reveals that the lubricating nanofiber group has a significantly lower adhesion score and a better antitissue adhesion. Altogether, our developed hydration-enhanced lubricating nanofibers show promising applications in the biomedical field such as antiadhesive membranes.

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“…MSCs seeded in nanofibrous meniscus scaffolds demonstrated fibrocartilage tissue growth that improved biomechanical properties at the knee and protected cartilage from degeneration in an animal model 68. Shimomura et al found similar results using a MSC-seeded nanofibrous scaffold constructed to match the meniscus circumferential fibre structure, and they demonstrated a significant decrease in meniscus extrusion following repair 71. Conversely, Hong et al 72 did not find evidence of tissue healing or regeneration with bone marrow stem cell matrix implantation in animal medial meniscus root tears.…”

Section: Bodymentioning

confidence: 92%

Biological augmentation to promote meniscus repair: from basic science to clinic application—state of the art

Strother

Saris

Verdonk³

et al. 2020

Journal of ISAKOS

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Enhanced repair of meniscal hoop structure injuries using an aligned electrospun nanofibrous scaffold combined with a mesenchymal stem cell-derived tissue engineered construct

Cited by 59 publications

References 47 publications

Single-cell RNA-seq analysis identifies meniscus progenitors and reveals the progression of meniscus degeneration

Single-cell RNA-seq analysis identifies meniscus progenitors and reveals the progression of meniscus degeneration

Hydration-Enhanced Lubricating Electrospun Nanofibrous Membranes Prevent Tissue Adhesion

Biological augmentation to promote meniscus repair: from basic science to clinic application—state of the art

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