Enhanced release of tanshinones and phenolics by nonionic surfactants from Salvia miltiorrhiza hairy roots

Abstract

Practical applicationSalvia miltiorrhiza root or Danshen is a well-known and valuable Chinese herb. Danshen contains two major classes of bioactive constituents, diterpenoid tanshinones and phenolic acids. These intracellular metabolite products are mainly retained within the S. miltiorrhiza hairy roots. In this study, several non-ionic surfactants were applied as membrane permeabilization agents to the S. miltiorrhiza hairy root culture, some of which were found effective to stimulate the release of tanshinones and phenolics from the hairy roots to the culture medium. The enhanced release of the intracellular products may facilitate their recovery from the culture system and also improve their production.www.els-journal.com Page 3 Engineering in Life SciencesThis article is protected by copyright. All rights reserved. AbstractThe development of effective measures for induction and stimulation of the secretion of intracellular products is an important strategy for improving the productivity and processing efficiency of plant tissue and cell cultures. In this study, several non-ionic surfactants were applied as membrane permeabilization agents to the Salvia miltiorrhiza hairy root culture to stimulate the release of intracellular secondary metabolites. Tween 20 and Triton X-100 were most effective to stimulate the secretion of tanshinones and phenolics. With 1% Tween 20(w/v), the total phenolic content (water soluble) released to the medium was increased from below 10% to over 50% and the total tanshinone increased from below 3% to over 70% of the total amount in culture. Some of the surfactants also promoted the production yields of tanshinones and phenolics. The hairy root growth (dry weight) was slightly increased by some of these surfactants. The addition of suitable surfactants to the S. miltiorrhiza hairy root culture has been shown effective to stimulate the release of useful metabolites from the hairy roots to facilitate their recovery from the culture.

Exploring plant tissue culture to improve the production of phenolic compounds: A review

“…The production of phenolic compounds is the main goal of the vast majority of those studies (Andarwulan and Shetty, 1999;Lozovaya et al, 2000;Santos-Gomes et al, 2003;Gális et al, 2004;Lozovaya et al, 2006;Kouakou et al, 2007;Bairu et al, 2011;Cui et al, 2011;Krzyzanowska et al, 2011;Palacio et al, 2012;Szopa et al, 2013;Siu et al, 2014;Szopa and Ekiert, 2014;Yildirim and Turker, 2014). However, some of them are also focused on the study of the extract's bioactive properties, such as antioxidant (Grzegorczyk et al, 2007;Hakkim et al, 2007;Kovatcheva-Apostolova et al, 2008;Hussein et al, 2010;Amoo et al, 2012;Giri et al, 2012;Khateeb et al, 2012; 16 Barros et al, 2013;Bhagya and Chandrashekar, 2013;Cheniany et al, 2013;Madhu, 2013;Goyali et al, 2014;Lugato et al, 2014;Piątczak et al, 2014;Valdez-Tapia et al, 2014), antimicrobial (Hussein et al, 2010;Ncube te al., 2011;Zhao et al, 2011;Khateeb et al, 2012) and cytotoxic (Skorić et al, 2012) activities.…”

Promotion of phenolic compounds production in Salvia miltiorrhiza hairy roots by six strains of rhizosphere bacteria

“…Meanwhile, the results showed that all six strains were able to increase the contents of RA and SAB in the hairy roots of S. miltiorrhiza . The increase rate of phenolic acids production induced by strain LNHR13 (the best strain) was less significantly than that by some signal molecules, such as ethylene and SA , whereas better than other elicitors, such as heavy metal salts (Ag + ) , nonionic surfactants (Tween 20 and Triton X‐100) and yeast extract . Above all, the evidence about phenolic acids content promoted by biotic elicitors is rarely available, and even most of the biotic elicitors (both live microbes and constituents of microbial cells) inhibit the biosynthesis of phenolic acids .…”

“…Salvia miltiorrhiza Bunge (Lamiaceae), commonly known as Danshen, is an important herb in China . Its rhizomes, having been widely used in the treatment of cerebrovascular and cardiovascular diseases in China as well as other countries, contain two types of bioactive substances, namely, phenolic acids and tanshinones .…”

Biosynthesis of bioactive ingredients of Salvia miltiorrhiza and advanced biotechnologies for their production

“…Tissue culture provided the first bioactive components of S. miltiorrhiza in the 1980s. Today, the yield of secondary metabolism has been remarkably improved by polyploid induction, callus culture [64], transformed cell suspension culture and hairy root culture [5].…”