Enhanced recovery and isolation of Campylobacter spp. from water using a novel device

Abstract

Aim: To design a special device which can be used with nonselective blood-free nutrient agar without enrichment for detection of campylobacters from water. Methods and Results: The Kapadnis-Baseri device (KB device) was designed and evaluated in comparison with the conventional method (C method) for detection of Campylobacter spp. from river water samples. The results indicated that the recovery of Campylobacter spp. by KB device was relatively more than by C method. Conclusions: To date, the methods for recovery of campylobacters are time consuming and involve use of special culture media, which is cost ineffective. The KB device is designed based on two important characters of Campylobacter, viz. motility and activity at low temperature. With this device we isolated Campylobacter spp. from river water on nonselective media without enrichment. Thus this device is as effective as the use of antibiotic media for the isolation of campylobacters. Significance and Impact of the Study: The KB device will be useful for isolation of Campylobacter spp. from water and other environmental samples, which is less time consuming and inexpensive. Besides, this device allows isolation of antibiotic sensitive campylobacters.

Development of a novel triplex PCR assay for the detection and differentiation of thermophilic species of Campylobacter using 16S-23S rDNA internal transcribed spacer (ITS) region

“…Thermophilic Campylobacter species are important causative agents of gastrointestinal infections in humans throughout the world (Penner 1988; Burnens and Nicolet 1993; Baserisalehi et al. 2004).…”