Environmental samples were subjected to determine frequency of occurrence of pathogenic campylobacters in the environment. The antimicrobial susceptibility of the isolates was tested to evaluate the level of antibiotic sensitive campylobacters in the environment of investigation. In all, 70 Campylobacter isolates were obtained from water and domestic animal faeces samples using Kapadnis-Baseri device and antimicrobial susceptibility of them was determined by disc diffusion test and E- test. The results indicated that all the isolates of Campylobacter were sensitive to ciprofloxacin and resistant to cefotaxime, cephalexin and ampicillin. Lowest MIC values were observed for ciprofloxacin and gentamicin (2 microg/mL) and highest MIC values for ampicillin and chloramphinicol (256 microg/mL). In general, pathogenic Campylobacter spp. were prevalent in large numbers in the environment, however, they were sensitive to ciprofloxacin.
Aim: To design a special device which can be used with nonselective blood-free nutrient agar without enrichment for detection of campylobacters from water. Methods and Results: The Kapadnis-Baseri device (KB device) was designed and evaluated in comparison with the conventional method (C method) for detection of Campylobacter spp. from river water samples. The results indicated that the recovery of Campylobacter spp. by KB device was relatively more than by C method. Conclusions: To date, the methods for recovery of campylobacters are time consuming and involve use of special culture media, which is cost ineffective. The KB device is designed based on two important characters of Campylobacter, viz. motility and activity at low temperature. With this device we isolated Campylobacter spp. from river water on nonselective media without enrichment. Thus this device is as effective as the use of antibiotic media for the isolation of campylobacters. Significance and Impact of the Study: The KB device will be useful for isolation of Campylobacter spp. from water and other environmental samples, which is less time consuming and inexpensive. Besides, this device allows isolation of antibiotic sensitive campylobacters.
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