Recovery of Campylobacter jejuni from surfaces of poultry slaughterhouses after cleaning and disinfection procedures: Analysis of a potential source of carcass contamination

Peyrat, Marie-Bénédicte; Soumet, Christophe; Maris, P.; Sandérs, Pascal

doi:10.1016/j.ijfoodmicro.2008.03.030

Cited by 79 publications

(57 citation statements)

References 30 publications

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“…isolated from broiler slaughterhouses. This observation suggests that such genotypes may be particularly adapted to survive cleaning and disinfection stress in poultry slaughterhouses and may contaminate the carcasses during processing (Peyrat et al 2008). Additionally, our results showed that Campylobacter may easily contaminate poultry carcasses at slaughter process.…”

Section: Resultsmentioning

confidence: 59%

Resistance to β-lactam and tetracycline in Campylobacter spp.isolated from broiler slaughterhouses in southern Brazil

et al. 2015

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RESUMO.-[Resistência a β-lactâmicos e tetraciclina emCampylobacter spp. isolados de matadouros-frigorífi-cos de aves no sul do Brasil.] O presente estudo foi realizado para examinar e analisar as características genéticas de resistência antimicrobiana de Campylobacter spp. a partir de fontes avícolas. Um total de 141 amostras de Campylobacter isolados em matadouros-frigoríficos de aves do estado do Rio Grande do Sul, Brasil, foi identificado por mé-todos fenotípicos e genotípicos. Foi analisada a susceptibilidade antimicrobiana e a presença de genes de resistência.As cepas foram testadas para detectar sensibilidade frente a dois antimicrobianos (ampicilina e tetraciclina) pelo método de difusão em disco. A seguir, usando a reação em cadeia da polimerase foi confirmada a espécie e a presença dos genes de resistência à ampicilina (bla OXA-61 ) e tetraciclina tet(O), assim como a detecção da bomba de efluxo (cmeB). Campylobacter jejuni foi a espécie mais isolada, sua presença foi determinada em 140 amostras (99,3%), e Campylobacter coli foi encontrada em uma única amostra (0,70%). Os resultados obtidos mostraram 65% e 35,5% de Campylobacter isolados resistentes a β-lactâmicos e tetraciclinas, respectivamente. O gene cmeB responsável pela resistência a múltiplos antimicrobianos foi detectado em 26 amostras (18,5%). Neste contexto, 36 das 141 amostras (25,6%) foram consideradas resistentes a dois grupos diferentes de antimicrobianos (β-lactâmicos e tetraciclinas). Campylobacter isolated from samples of broilers of slaughterhouses in southern Brazil was identified by phenotypic and genotypic methods. Campylobacter isolates were evaluated for its antimicrobial susceptibility and the presence of resistance genes. The strains were investigated for antimicrobial susceptibility against two agents (ampicillin and tetracycline) by disk diffusion method. PCR assay was used to confirm the specie and the presence of ampicillin (bla OXA-61 ), tetracycline tet(O), and the energy-dependent multi-drug efflux pump (cmeB) genes. Campylobacter jejuni was the most ubiquitous; its presence was determined in 140 samples out of 141 (99.3%), whereas Campylobacter coli was found only in one of the contaminated samples (0.70%). The results obtained showed 65% and 35.5% of Campylobacter isolates resistant to β-lactams and tetracyclines, respectively. The cmeB gene responsible for multidrug resistance was detected in 26 isolates out 141 strains (18.5%). Moreover, 36 out of 141 Campylobacter strains (25.6%) were found to be resistant to at least two different antimicrobia resistance markers (β-lactams and tetracyclines). INDEX TERMS:Campylobacter, tetracycline, β-lactam, efflux pump, resistance genes, PCR. Yuli M. Sierra-Arguello et al.

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Section: Resultsmentioning

confidence: 59%

Resistance to β-lactam and tetracycline in Campylobacter spp.isolated from broiler slaughterhouses in southern Brazil

et al. 2015

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“…Campylobacter-colonized flocks contaminate the abattoir environment upon entering the room and as a consequence Campylobacter can be isolated at all stages of the processing line (Ellerbroek et al 2010). C. jejuni is able to survive overnight on these processing equipment surfaces, even after cleaning and disinfection (Peyrat et al 2008). Therefore, surviving strains might possibly be a source of poultry carcass contamination of subsequent flocks, probably by intestinal contents of previously processed Campylobacter-colonized flocks (Newell et al 2001, Miwa et al 2003.…”

Section: Carcass Contaminationmentioning

confidence: 99%

Poultry as a Host for the Zoonotic PathogenCampylobacter jejuni

Hermans

Pasmans²,

Messens³

et al. 2012

Vector-Borne and Zoonotic Diseases

221

157

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Campylobacteriosis is the most reported foodborne gastroenteritic disease and poses a serious health burden in industrialized countries. Disease in humans is mainly caused by the zoonotic pathogen Campylobacter jejuni. Due to its wide-spread occurrence in the environment, the epidemiology of Campylobacter remains poorly understood. It is generally accepted, however, that chickens are a natural host for Campylobacter jejuni, and for Campylobacter spp. in general, and that colonized broiler chicks are the primary vector for transmitting this pathogen to humans. Several potential sources and vectors for transmitting C. jejuni to broiler flocks have been identified. Initially, one or a few broilers can become colonized at an age of > 2 weeks until the end of rearing, after which the infection will rapidly spread throughout the entire flock. Such a flock is generally colonized until slaughter and infected birds carry a very high C. jejuni load in their gastrointestinal tract, especially the ceca. This eventually results in contaminated carcasses during processing, which can transmit this pathogen to humans. Recent genetic typing studies showed that chicken isolates can frequently be linked to human clinical cases of Campylobacter enteritis. However, despite the increasing evidence that the chicken reservoir is the number one risk factor for disease in humans, no effective strategy exists to reduce Campylobachter prevalence in poultry flocks, which can in part be explained by the incomplete understanding of the epidemiology of C. jejuni in broiler flocks. As a result, the number of human campylobacteriosis cases associated with the chicken vector remains strikingly high.

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“…Many of these concerns stem from practices common to IFAP, such as the continued use of arsenical drugs in poultry production (110), the use of hormones in beef and dairy production, and the widespread administration of antimicrobials (see the section entitled Antimicrobial Resistance). Animal slaughtering and meat-processing facilities present further multiple opportunities for microbial cross-contamination (77,119), which are heightened by the volume of carcasses and the speed at which they are processed.…”

Section: Microbial and Chemical Contaminationmentioning

confidence: 99%

Food System Policy, Public Health, and Human Rights in the United States

Shannon

Kim

McKenzie

et al. 2015

Annu. Rev. Public Health

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The US food system functions within a complex nexus of social, political, economic, cultural, and ecological factors. Among them are many dynamic pressures such as population growth, urbanization, socioeconomic inequities, climate disruption, and the increasing demand for resourceintensive foods that place immense strains on public health and the environment. This review focuses on the role that policy plays in defining the food system, particularly with regard to agriculture. It further examines the challenges of making the food supply safe, nutritious, and sustainable, while respecting the rights of all people to have access to adequate food and to attain the highest standard of health. We conclude that the present US food system is largely unhealthy, inequitable, environmentally damaging, and insufficiently resilient to endure the impacts of climate change, resource depletion, and population increases, and is therefore unsustainable. Thus, it is imperative that the US embraces policy reforms to transform the food system into one that supports public health and reflects the principles of human rights and agroecology for the benefit of current and future generations.

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Recovery of Campylobacter jejuni from surfaces of poultry slaughterhouses after cleaning and disinfection procedures: Analysis of a potential source of carcass contamination

Cited by 79 publications

References 30 publications

Resistance to β-lactam and tetracycline in Campylobacter spp.isolated from broiler slaughterhouses in southern Brazil

Resistance to β-lactam and tetracycline in Campylobacter spp.isolated from broiler slaughterhouses in southern Brazil

Poultry as a Host for the Zoonotic PathogenCampylobacter jejuni

Food System Policy, Public Health, and Human Rights in the United States

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