Enhanced production of xylanase from locally isolated fungal strain using agro-industrial residues under solid-state fermentation

Abdullah, Rasedee; Nisar, Kinza; Aslam, Aafia; Iqtedar, Mehwish; Naz, Shagufta

doi:10.1080/14786419.2014.968157

Cited by 8 publications

(6 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, as this commercial extract was diluted ten times for the investigations in this study, the actual activity was 7.8 U mL –1 . Suwannarangsee et al ., 18 and Abdullah et al 26 . also reported the activity profile of Celluclast™ using a proteomic analysis with liquid chromatography–tandem mass spectrometry (LC/MS/MS).…”

Section: Resultsmentioning

confidence: 99%

Screening and cocktail optimization using experimental mixture design: enzymatic saccharification as a biological pretreatment strategy

Pinheiro

Horváth

Lundin

et al. 2021

Biofuels Bioprod Bioref

View full text Add to dashboard Cite

Biofuels contribute environment‐friendly, renewable energy, minimizing dependence on fossil energy. The pretreatment of biomass is a practical step that accelerates and facilitates the hydrolysis of complex materials. This work aimed to screen, select, and study cocktail formulations for biomass hydrolysis, where the enzymes were provided both from a commercial source (Trichoderma reesei, Novozymes®) and through the cultivation of Aspergillus brasiliensis and Aspergillus tamarii Kita. Experimental mixture designs were used to optimize the enzymatic conversion of substrates into simple sugars. A crude extract rich in amylase (AAB) had a significant favorable influence on cornmeal hydrolysis by maximizing the yield of reducing sugars (RS) (173 μmol mL–1). Celluclast™, rich in cellulase, significantly affected the hydrolysis of banana peel, maximizing the RS yield (175 μmol mL–1). Variable degrees of enzyme synergism were evident from statistical analysis of the biomass hydrolysis. © 2021 Society of Chemical Industry and John Wiley & Sons, Ltd

show abstract

Section: Resultsmentioning

confidence: 99%

Screening and cocktail optimization using experimental mixture design: enzymatic saccharification as a biological pretreatment strategy

Pinheiro

Horváth

Lundin

et al. 2021

Biofuels Bioprod Bioref

View full text Add to dashboard Cite

show abstract

“…Raw EFB presents cellulose, hemicellulose, and lignin fractions of 42.2%, 29.4%, and 13.8%, respectively. Different EFB compositions are reported that are related to a different temperature, humidity, soil composition, and types of crops .…”

Section: Resultsmentioning

confidence: 99%

Sequential chemical and enzymatic pretreatment of palm empty fruit bunches for Candida pelliculosa bioethanol production

Campioni

Soccol

Libardi

et al. 2019

Biotech and App Biochem

View full text Add to dashboard Cite

Second-generation bioethanol production process was developed using pretreated empty fruit bunches (EFB). Consecutive acid/alkali EFB pretreatment was performed, first with HCl and then with NaOH with final washing steps for phenolic compounds elimination. Scanning electron microscopy images showed that EFB chemical treatments indeed attacked the cellulose fibers and removed the silica from surface pores. The optimization of enzymatic hydrolysis of EFB's cellulosic fraction was performed with 0.5%-4% v/v of Cellic R CTec2/Novozymes, different EFB concentrations (5%-15%, w/v), and hydrolysis time (6-72 H). Optimization essays were carried out in Erlenmeyer flasks and also in a 1 L stirred tank reactor. After enzymatic hydrolysis, a hydrolysate with 66 g/L of glucose was achieved with 2.2% (v/v) Cellic R CTec2, 15% (m/v) acid/alkaline pretreated EFB after 39 H of hydrolysis. A gain of 11.2% was then obtained in the 1 L stirred tank promoted by the agitation (72.2 g/L glucose). The hydrolysate was employed in bioethanol production by a new isolate Candida pelliculosa CCT 7734 in a separate hydrolysis and fermentation process reaching 16.6 and 23.0 g/L of bioethanol through batch and fed-batch operation, respectively. An integrated biorefinery process was developed for EFB processing chain.

show abstract

“…Isolation of fungal cultures with xylanase activity was made from different sources such as active compost, decaying organic materials, and soil samples (Saha and Ghosh, 2014 ; Adhyaru et al, 2015 ; Pereira et al, 2015 ). Senthil et al ( 2005 ), Abdullah et al ( 2014 ), and Bekkarevich et al ( 2015 ) reported xylanase activity of fungal and bacterial cultures within a range of 182–3060 U.…”

Section: Discussionmentioning

confidence: 99%

Optimization of Xylanase Production through Response Surface Methodology by Fusarium sp. BVKT R2 Isolated from Forest Soil and Its Application in Saccharification

Ramanjaneyulu

Reddy

2016

Front. Microbiol.

View full text Add to dashboard Cite

Xylanses are hydrolytic enzymes with wide applications in several industries like biofuels, paper and pulp, deinking, food, and feed. The present study was aimed at hitting at high yield xylanase producing fungi from natural resources. Two highest xylanase producing fungal isolates—Q12 and L1 were picked from collection of 450 fungal cultures for the utilization of xylan. These fungal isolates—Q12 and L1 were identified basing on ITS gene sequencing analysis as Fusarium sp. BVKT R2 (KT119615) and Fusarium strain BRR R6 (KT119619), respectively with construction of phylogenetic trees. Fusarium sp. BVKT R2 was further optimized for maximum xylanase production and the interaction effects between variables on production of xylanase were studied through response surface methodology. The optimal conditions for maximal production of xylanase were sorbitol 1.5%, yeast extract 1.5%, pH of 5.0, Temperature of 32.5°C, and agitation of 175 rpm. Under optimal conditions, the yields of xylanase production by Fusarium sp. BVKT R2 was as high as 4560 U/ml in SmF. Incubation of different lignocellulosic biomasses with crude enzyme of Fusarium sp. BVKT R2 at 37°C for 72 h could achieve about 45% saccharification. The results suggest that Fusarium sp. BVKT R2 has potential applications in saccharification process of biomass.

show abstract

Enhanced production of xylanase from locally isolated fungal strain using agro-industrial residues under solid-state fermentation

Cited by 8 publications

References 15 publications

Screening and cocktail optimization using experimental mixture design: enzymatic saccharification as a biological pretreatment strategy

Screening and cocktail optimization using experimental mixture design: enzymatic saccharification as a biological pretreatment strategy

Sequential chemical and enzymatic pretreatment of palm empty fruit bunches for Candida pelliculosa bioethanol production

Optimization of Xylanase Production through Response Surface Methodology by Fusarium sp. BVKT R2 Isolated from Forest Soil and Its Application in Saccharification

Contact Info

Product

Resources

About