Optimization of Xylanase Production through Response Surface Methodology by Fusarium sp. BVKT R2 Isolated from Forest Soil and Its Application in Saccharification

Abstract

Xylanses are hydrolytic enzymes with wide applications in several industries like biofuels, paper and pulp, deinking, food, and feed. The present study was aimed at hitting at high yield xylanase producing fungi from natural resources. Two highest xylanase producing fungal isolates—Q12 and L1 were picked from collection of 450 fungal cultures for the utilization of xylan. These fungal isolates—Q12 and L1 were identified basing on ITS gene sequencing analysis as Fusarium sp. BVKT R2 (KT119615) and Fusarium strain BRR R6 (KT119619), respectively with construction of phylogenetic trees. Fusarium sp. BVKT R2 was further optimized for maximum xylanase production and the interaction effects between variables on production of xylanase were studied through response surface methodology. The optimal conditions for maximal production of xylanase were sorbitol 1.5%, yeast extract 1.5%, pH of 5.0, Temperature of 32.5°C, and agitation of 175 rpm. Under optimal conditions, the yields of xylanase production by Fusarium sp. BVKT R2 was as high as 4560 U/ml in SmF. Incubation of different lignocellulosic biomasses with crude enzyme of Fusarium sp. BVKT R2 at 37°C for 72 h could achieve about 45% saccharification. The results suggest that Fusarium sp. BVKT R2 has potential applications in saccharification process of biomass.

Optimized Production of Xylanase by Penicillium purpurogenum and Ultrasound Impact on Enzyme Kinetics for the Production of Monomeric Sugars From Pretreated Corn Cobs

“…Apart from biorefinery, xylanases have a potential industrial application in various fields like wine making, textile, paper and pulp industries, animal husbandry, bread making, fruit juice extraction [7] etc. Production of xylanases in large scale is gaining much importance in getting the scope to cut down the enzyme cost with the use of high yielding microbes [8]. Xylanase production by various organisms have been studied like fungi [7], bacteria [9], yeast, [10], and even with co-culture of more than one organism [11].…”

Optimized Production of Xylanase by Penicillium purpurogenum and Ultrasound Impact on Enzyme Kinetics for the Production of Monomeric Sugars From Pretreated Corn Cobs

“…Apart from biorefinery, xylanases have a potential industrial application in various fields like wine making, textile, paper and pulp industries, animal husbandry, bread making, fruit juice extraction (Chadha et al, 2019) etc. Production of xylanases in large scale is gaining much importance in getting the scope to cut down the enzyme cost with the use of high yielding microbes (Ramanjaneyulu and Rajasekhar Reddy, 2016). Xylanase production by various organisms have been studied like fungi (Chadha et al, 2019), bacteria (Kumar et al, 2018), yeast, (Ergun and Çalık, 2016), and even with co-culture of more than one organism (Yardimci and Cekmecelioglu, 2018).…”

Harnessing Mucor spp. for Xylanase Production: Statistical Optimization in Submerged Fermentation Using Agro-Industrial Wastes

“…RSM method by Azzouz et al [ 39 ] reported a 65.01% increase in xylanase output by Aspergillus niger strain BG on 84% humidified wheat bran with a pH of 2.5 at 37°C and incubation for 66 h. Statistical analysis of xylanase output by Aspergillus foetidus in submerged fermentation with soybean scraps revealed that variables pH and the interaction of pH and temperature influenced xylanase biosynthesis, with good xylanase activity (13.98 U/mL) at pH 7.0, 28 °C, and 120 rpm for 168 hours [ 40 ]. Response surface methodology by Ramanjaneyulu and Rajasekhar [ 41 ] for maximal production of xylanase (4560 U/mL) by Fusarium sp. BVKT R2 demonstrated that the optimal conditions were sorbitol 1.5%, yeast extract 1.5%, pH of 5.0, temperature of 32.5 °C, and shaking of 175 rpm.…”