Optimized Production of Xylanase by Penicillium purpurogenum and Ultrasound Impact on Enzyme Kinetics for the Production of Monomeric Sugars From Pretreated Corn Cobs

Abstract

All the experiments were designed and carried out by BS with the help of BK. BB designed the concept, monitored all the experiments, and drafted the manuscript. All authors read and approved the final manuscript.

Assessment of indigenous fungal biocatalysts towards valorization of delignified physico‐chemically pretreated corn cobs and sugarcane bagasse

“…A similar effect of acid/alkali treatment has been reported earlier in corn cobs and sugarcane bagasse by many researchers 8,30–32 . Our study reports similar rates of lignin removal through alkali treatment in less time and with a reduced chemical dose (NaOH) 11,13,33 . This may be advantageous in industrial‐scale process optimization aiming at minimal operation time and chemical costs.…”

“…The present study reports better saccharification of agro‐residues than the studies by Thite and Nerurkar 8 (15.6% saccharification from ALB) and Sunkar et al . (58.88% saccharification of hemicelluloses from ALC; 183.42 ± 4.13 mg/g xylose; enzyme loading 2000 U per gram dry substrate, gds) in a 48 h reaction time 11 . Similarly, Garai and Kumar reported the production of 190.66 mg/g xylose (CC) and 299.88 mg/g reducing sugars (SB) from 15% ammonium‐pretreated residues using crude xylanase (750 U/gds) from Aspergillus candidus , 12 whereas Chapla et al .…”

“…5). This might be due to the saturation of substrate concentration and therefore the enzyme to substrate ratio needs to be optimized for a productive enzyme hydrolysis 11 . Moreover, the effectiveness of enzyme hydrolysis and the pretreatment technique also varies for each biomass owing to their structural and compositional diversity 13 …”

“…Physico‐chemical pretreatments integrate chemical (sodium hydroxide, potassium hydroxide, sulfuric acid, hydrochloric acid, liquid ammonia) and physical parameters (pressure, heat, microwave, sonication), thereby improving the accessibility of glycosidic linkages toward biomass deconstruction 7,9 . Several studies have reported the use of indigenous as well as commercial enzyme preparations (such as Primafast 200, CTec2, Multifect xylanase) after different pretreatments for enhanced enzymatic saccharification of biomass, yet none have been optimized for maximum efficiency at the commercial level 8,10,11 …”