Enhanced pro-coagulant hemostatic agents based on nanometric zeolites

Laurenti, Juliana Bergamasco; Zazeri, Gabriel; Povinelli, Ana Paula Ribeiro; Godoy, Moacir Fernandes de; Braile, Domingo Marcolino; Rocha, Tânia Rúbia Flores da; Amico, Élbio Antônio D'; Nery, José Geraldo

doi:10.1016/j.micromeso.2016.10.020

Cited by 18 publications

(10 citation statements)

References 47 publications

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“…TEG is a global evaluation of blood stasis by quantitative measurement of viscoelastic changes in the blood. Table presents the TEG parameters, where R is the time from the start of the test to the initiation of fibrin formation of clot to an amplitude of 2 mm, K is the time for the clot to reach an amplitude of 20 mm, α angle is a measure of the speed at which fibrin build up occurs, and MA is the maximum amplitude that reflects the maximum elastic property of fibrin and platelet adhesion . To prove and identify the most remarkable hemostatic agent in vitro, TEG tests were also performed on CS, CS6b, CS12b, and CS18a, and the results are shown in Figure B.…”

Section: Resultsmentioning

confidence: 99%

Exploration of Blood Coagulation of N-Alkyl Chitosan Nanofiber Membrane in Vitro

et al. 2018

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N-Alkylated chitosan (NACS) may improve the blood clotting efficiency of chitosan (CS). To study its blood coagulation capability, a series of NACSs with various carbon chain lengths and degrees of substitution (DS) of alkyl groups were synthesized and characterized by FTIR, NMR, elemental analysis, and X-ray diffraction (XRD). The corresponding NACS nanofiber membranes (NACS-NM) were subsequently fabricated by electronic spinning technique. SEM, XRD, DSC, surface area, porosity, contact angle, blood absorption, and mechanical properties were used to characterize the CS-NM/NACS-NM. Moreover, cytotoxicity, coagulation, activated partial thromboplastin time, plasma prothrombin time, thrombin time, and platelet aggregation tests were performed to evaluate the biocompatibility and blood coagulation properties of NACS-NM. The results showed that NACS-NM was not cytotoxic. NACS-NM with DS of 19.25% for N-hexane CS (CS6b), 17.87% for N-dodecane CS (CS12b), and 8.97% for N-octadecane CS (CS18a) exhibited good blood clotting performance. Moreover, NACS-NMs favored the activation of coagulation factors and platelets. In addition, intracellular Ca was not related to platelet activation. The above results suggested that NACS-NM would be an effective hemostatic agent.

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Section: Resultsmentioning

confidence: 99%

Exploration of Blood Coagulation of N-Alkyl Chitosan Nanofiber Membrane in Vitro

et al. 2018

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“…Currently, surgical suture is the only clinical method for aortic rupture and heart wound sealing; however, this is not feasible outside surgical units and is not attempted in most emergency situations 2 . With recent progress in materials science, many experimental chemical agents have been tested for rapid wound sealing, such as fibrin glue, gelatin, collagen, oxidized cellulose, zeolites, peptides, polymers, and hydrogels 3–9 . However, none of these materials are suitable for aortic and heart trauma hemostasis and sealing because of their slow hemostatic performance, poor wet tissue surface adhesion, and weak or inflexible bonding mechanics.…”

Section: Introductionmentioning

confidence: 99%

A strongly adhesive hemostatic hydrogel for the repair of arterial and heart bleeds

et al. 2019

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Uncontrollable bleeding is a major problem in surgical procedures and after major trauma. Existing hemostatic agents poorly control hemorrhaging from traumatic arterial and cardiac wounds because of their weak adhesion to wet and mobile tissues. Here we design a photo-reactive adhesive that mimics the extracellular matrix (ECM) composition. This biomacromolecule-based matrix hydrogel can undergo rapid gelling and fixation to adhere and seal bleeding arteries and cardiac walls after UV light irradiation. These repairs can withstand up to 290 mm Hg blood pressure, significantly higher than blood pressures in most clinical settings (systolic BP 60–160 mm Hg). Most importantly, the hydrogel can stop high-pressure bleeding from pig carotid arteries with 4~ 5 mm-long incision wounds and from pig hearts with 6 mm diameter cardiac penetration holes. Treated pigs survived after hemostatic treatments with this hydrogel, which is well-tolerated and appears to offer significant clinical advantage as a traumatic wound sealant.

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“…Zeolites, i.e., crystalline aluminosilicates, which are now widely used in various fields of industry, seem to be attractive materials for biomedical and tissue engineering applications. Examples of these applications include haemostatics [7], carriers for drug delivery [8,9], substrates for protein immobilization [10], substrates for incorporating metallic ions with antibacterial properties [11], and, particularly, bone implant coatings [12]. Various applications of zeolites, including applications with biomedical perspectives, have been summarized in recent reviews [13,14].…”

Section: Introductionmentioning

confidence: 99%

Silicalite-1 Layers as a Biocompatible Nano- and Micro-Structured Coating: An In Vitro Study on MG-63 Cells

et al. 2019

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Silicalite-1 is a purely siliceous form of zeolite, which does not contain potentially harmful aluminum in its structure as opposed to ZSM-5 aluminosilicate types of zeolite. This paper reports on a study of a silicalite-1 film, deposited on a silicon Si(100) substrate, as a potential anti-corrosive and biocompatible coating for orthopaedic implants. Silicalite-1 film was prepared in situ on the surface of Si(100) wafers using a reaction mixture of tetrapropyl-ammonium hydroxide (TPAOH), tetraethyl-orthosilicate (TEOS), and diH2O. The physico-chemical properties of the obtained surface were characterized by means of X-ray photoelectron spectroscopy, water contact angle measurement, atomic force microscopy, and scanning electron microscopy. The biocompatibility was assessed by interaction with the MG-63 cell line (human osteosarcoma) in terms of cell adhesion, morphology, proliferation, and viability. The synthesized silicalite-1 film consisted of two layers (b- and a, b-oriented crystals) creating a combination of micro- and nano-scale surface morphology suitable for cell growth. Despite its hydrophobicity, the silicalite-1 film increased the number of initially adhered human osteoblast-like MG-63 cells and the proliferation rate of these cells. The silicalite-1 film also improved the cell viability in comparison with the reference Si(100) substrate. It is therefore a promising candidate for coating of orthopaedic implants.

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Enhanced pro-coagulant hemostatic agents based on nanometric zeolites

Cited by 18 publications

References 47 publications

Exploration of Blood Coagulation of N-Alkyl Chitosan Nanofiber Membrane in Vitro

Exploration of Blood Coagulation of N-Alkyl Chitosan Nanofiber Membrane in Vitro

A strongly adhesive hemostatic hydrogel for the repair of arterial and heart bleeds

Silicalite-1 Layers as a Biocompatible Nano- and Micro-Structured Coating: An In Vitro Study on MG-63 Cells

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