Enhanced plasma miR‑142‑5p promotes the progression of intrahepatic cholangiocarcinoma via targeting PTEN

Wei, Guifen; Yuan, Yiting; He, Xinzhong; Jin, Liming; Jin, Di

doi:10.3892/etm.2019.7438

Cited by 5 publications

(7 citation statements)

References 37 publications

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“…dysregulated expression or function of miRNAs has been demonstrated in a series of malignancies, including ccA (25). For example, miR-142-5p was shown to decrease the expression of phosphatase and tensin homolog and promote the progression of ccA (26). It was demonstrated that miR-329 can suppress the expression of pituitary tumor-transforming 1, inactivates the mitogen-activated protein kinase/extracellular signal-regulated kinase signaling pathway and has the potential to suppress ccA cell proliferation (27).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‑137 suppresses the proliferation, migration and invasion of cholangiocarcinoma cells by targeting WNT2B

et al. 2020

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It is widely known that abnormal regulation of microRNAs (miRNAs/miRs) may contribute to the occurrence or development of tumors. The objective of the present study was to elucidate the function and underlying mechanism of miR-137 in the progression of cholangiocarcinoma (ccA). The expression levels of miR-137 in ccA tissues and cell lines were measured using reverse transcription-quantitative PcR. The role of miR-137 in the proliferation of ccA cells was assessed using the cell counting Kit-8 assay, colony formation assay and cell cycle distribution analysis, while its effects on the migration and invasion of ccA cells were evaluated using Transwell assays. The function of miR-137 on ccA growth in vivo was also investigated using a xenograft mouse model. Furthermore, the association between miR-137 and Wnt family member 2B (WNT2B) was analyzed using bioinformatics, double luciferase assay and western blotting. It was verified that the expression of miR-137 was low in ccA tissues and cell lines, whereas increased expression of miR-137 significantly suppressed cell proliferation, decreased colony formation ability and induced G1 phase arrest. miR-137 overexpression suppressed the migration and invasion ability of TFK-1 and HuccT1 cells. Furthermore, the results of the xenograft mouse model assays revealed that miR-137 overexpression decreased tumor growth in vivo. The results of bioinformatics analysis and dual luciferase reporter assays demonstrated that WNT2B is directly regulated by miR-137. The expression of WNT2B and Wnt-pathway-related proteins was decreased when miR-137 was overexpressed. Restoring the expression of WNT2B notably reversed the inhibitory effect of miR-137 on CCA cells. Therefore, the findings of the present study demonstrated that miR-137 acts as a suppressor in ccA and inhibits ccA cell proliferation, migration and invasion through suppressing the expression of WNT2B.

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Section: Discussionmentioning

confidence: 99%

MicroRNA‑137 suppresses the proliferation, migration and invasion of cholangiocarcinoma cells by targeting WNT2B

et al. 2020

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“…The included articles were published between 2011 and 2020. Fifteen and five articles were published in English [ 7–20 , 23 ] and Chinese [ 21,22 , 24–26 ], respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Five and fifteen articles detected the expression of miR-142 in blood and tissues, respectively. A total of 12 solid tumors were included, namely HCC [ 8 , 10 ], CRC [ 9 ], NSCLC [ 11 , 24 ], CC [ 12 , 15 , 21 , 26 ], NPC [ 13 ], EC [ 18 ], ESCC [ 14 , 22 ], RCC [ 16 ], glioma [ 17 ], ICC [ 7 ], GC [19,20,], melanoma [ 23 ] and OSCC [ 25 ]. The average NOS score of the included articles was 6.40.…”

Section: Resultsmentioning

confidence: 99%

“…Studies revealed that miR-142 participates in tumor cell proliferation, differentiation, invasion, and metastasis by regulating numerous genes, such as phosphatidylinositol-4,5-bisphosphate 3-kinase (PIK3CA), ras-related protein rap-1 A (RAP1A), Frizzled7 receptor (FZD7) and phosphatase and tensin homolog (PTEN) [ 4–7 ]. Recently, studies reported that the abnormal expression of miR-142 miRNAs is associated with the prognosis and clinical characteristics of many tumors, including hepatocellular carcinoma (HCC), colorectal cancer (CRC), non-small cell lung cancer (NSCLC), cervical cancer (CC), nasopharyngeal carcinoma (NPC), endometrial cancer (EC), esophageal squamous cell carcinoma (ESCC), renal cell carcinoma (RCC), intrahepatic cholangiocarcinoma (ICC), gastric cancer (GC), oral squamous cell carcinoma (OSCC) [ 7–26 ]. However, their results were inconsistent.…”

Section: Introductionmentioning

confidence: 99%

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Prognostic value of miR-142 in solid tumors: a meta-analysis

et al. 2021

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Several studies on the prognostic value of the microRNA 142 (miR-142) in solid tumors have reported conflicting results. Therefore, the aim of this meta-analysis was to evaluate the relationship between the miR-142 and prognosis in solid tumors. A comprehensive search for relevant studies was conducted until November 10, 2020. Studies that investigated the prognostic significance of the miR-142 in solid tumors were included. The hazard ratio and 95% confidence interval were calculated using a random-effects model. All data analyses were performed using the STATA 12.0 software (Stata Corporation, College Station, TX, USA).Twenty articles involving 2451 participants were included in the meta-analysis. The results showed that high miR-142 expression was a better predictor of overall survival (OS) (HR=0.66, 95% CI: 0.47–0.93) and disease-free/progression-free/recurrence-free survival (DFS/PFS/RFS) (HR=0.71, 95% CI: 0.55–0.91) compared with low miR-142 expression. MiR-142 can be used as an effective prognostic marker for patients with solid tumors. Future large prospective studies are warranted to further confirm the present findings.

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“…There have been several reports on the relationship between ICC tumor cells and miRNAs. Wei et al 23 reported that miR‐142‐5p promotes ICC tumor cells by targeting PTEN. Wu et al 24 also reported that miR‐424‐5p suppresses ICC tumor cells via ARK5.…”

Section: Discussionmentioning

confidence: 99%

Cancer‐associated fibroblasts promote tumor cell growth via miR‐493‐5p in intrahepatic cholangiocarcinoma

et al. 2022

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The association between tumor microenvironment (TME) and cancer‐associated fibroblasts (CAFs) in intrahepatic cholangiocarcinoma (ICC) progression is poorly understood. This study aimed to reveal whether specific microRNAs (miRNAs) in extracellular vesicles (EVs) derived from CAFs were involved in ICC progression. Conditioned medium (CM) and EVs in the CM of CAFs and normal fibroblasts (NFs) derived from ICC specimens were used to investigate the effects on tumor cell lines. miRNA microarray assay was used to examine the miRNAs of EVs derived from CAFs and NFs in ICC, and the effects of miR‐493‐5p on tumor cell lines were examined. Additionally, databases were used to identify miR‐493‐5p targets, and the relationship between prognosis of ICC patients and cocaine‐ and amphetamine‐regulated transcript propeptide (CARTPT), one of the targets of miR‐493‐5p, expression in ICC tissues was retrospectively analyzed. Compared with NF‐derived CM and EVs, CAF‐derived CM and EVs promoted cell lines in proliferation, scratch, migration, and invasion assays. miRNA microarray analysis revealed that miR‐493‐5p was significantly increased in CAF‐derived EVs compared to NF‐derived EVs. Tumor cell lines transfected with miR‐493‐5p were promoted in proliferation and scratch assays. Immunohistochemical staining was performed on 76 ICC specimens; both overall and recurrence‐free survival rates were significantly worse in the CARTPT‐negative group. Univariate and multivariate analyses showed that low CARTPT expression was an independent poor prognostic factor for overall and recurrence‐free survival. Overall, our data suggest that CAFs in the ICC TME suppress CARTPT in tumor cells and promote tumor cells via miR‐493‐5p in EVs.

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Enhanced plasma miR‑142‑5p promotes the progression of intrahepatic cholangiocarcinoma via targeting PTEN

Cited by 5 publications

References 37 publications

MicroRNA‑137 suppresses the proliferation, migration and invasion of cholangiocarcinoma cells by targeting WNT2B

MicroRNA‑137 suppresses the proliferation, migration and invasion of cholangiocarcinoma cells by targeting WNT2B

Prognostic value of miR-142 in solid tumors: a meta-analysis

Cancer‐associated fibroblasts promote tumor cell growth via miR‐493‐5p in intrahepatic cholangiocarcinoma

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