MicroRNA‑137 suppresses the proliferation, migration and invasion of cholangiocarcinoma cells by targeting WNT2B

Chen, Tengxiang; Lei, Shan; Zeng, Zhirui; Pan, Shutao; Zhang, Jinjuan; Xue, Yan; Sun, Yihong; Lan, Jinzhi; Xu, Shi‐Hai; Mao, Dahua; Guo, Bing

doi:10.3892/ijmm.2020.4474

Cited by 10 publications

(7 citation statements)

References 28 publications

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“…Preliminary studies have shown that Wnt2b is a highly conserved member of the WNT family [48]. Wnt2b was found dysregulated in several cancers with aberrant Wnt/β-catenin pathway activity [49][50][51][52][53]. In our study, we identified that Wnt2b is positively associated with osteogenesis by activating the Wnt/β-catenin signaling pathway for the first time.…”

Section: Discussionsupporting

confidence: 51%

PiRNA-63049 inhibits bone formation through Wnt/β-catenin signaling pathway

et al. 2021

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Section: Discussionsupporting

confidence: 51%

PiRNA-63049 inhibits bone formation through Wnt/β-catenin signaling pathway

et al. 2021

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“…Furthermore, analysis of hsa-miR-137 in the KM prognosis of cancer patients showed that hsa-miR-137 was a relatively favorable prognostic factor for many types of cancer, although not absolute. In previous studies, hsa-miR-137 has also been shown to be a tumor suppressor in Triple-negative breast cancer (TNBC), osteosarcoma cancer, oral cancer, STAD, COAD, cholangiocarcinoma cell and OV (Dang et al, 2013;Sakaguchi et al, 2016;Chen et al, 2018;Zhang et al, 2018;Chen et al, 2020a;Chen et al, 2020b;Weng and Wang, 2022). The negative correlation between BTLA and hsa-miR-137 suggests that BTLA may promote tumor progression by inhibiting the expression of hsa-miR-137.…”

Section: Discussionmentioning

confidence: 97%

A pan-cancer analysis of the biological function and clinical value of BTLA in tumors

Jiang¹,

He²,

WANG³

et al. 2023

Biocell

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B and T-lymphocyte attenuator (BTLA) plays an immunosuppressive role by inhibiting T-and B-cell functions.BTLA is associated with a variety of diseases, especially cancer immunity. However, the function of BTLA in various cancers and its clinical prognostic value have still not been comprehensively analyzed. This study aimed to identify the relationship between BTLA and cancer from the perspectives of differences in BTLA expression, its clinical value, immune infiltration, and the correlation with immune-related genes in various cancers. Data regarding mRNA expression, miRNA expression, lncRNA expression, and clinical data of patients of 33 existing cancers were collected from the TCGA database. Target miRNA of BTLA and the lncRNA that interacts with the target miRNA were obtained from the StarBase database. Based on bioinformatics analysis methods, the relationship between various types of cancers and BTLA was thoroughly investigated, and a competing endogenous RNA network of BTLA, target miRNA, and interacting lncRNA was constructed. The Kaplan-Meier (KM) prognostic analysis of BTLA and target miRNA (has-miR-137) in various types of cancers was completed using the KM plotter. BTLA expression varied in different cancers, with statistical significance in nine cancer types. KM plotter to analyze the overall survival (OS) and regression-free survival prognosis of cancer patients revealed that the BTLA expression was statistically different in the OS of 11 types of cancers out of 21 types of cancers; the OS of 8 type of cancers was also statistically different.Correlation analysis of tumor immune genes revealed a positive correlation of BTLA expression with immunosuppressive gene (CTLA4 and PDCD1) expression. Gene Set Enrichment Analysis showed that BTLA and its co-expressed genes mainly act through biological processes and pathways, including immune response regulation, cell surface receptor signaling pathway, antigen binding, antigen receptor-mediated signaling pathway, and leukocyte migration. BTLA has the potential as a prognostic marker for CLL, COAD, NSCLC, and OV and a diagnostic marker for CLL, COAD, and KIRC. BTLA has a close and complex relationship with the occurrence and development of tumors, and cancer immunotherapy for BTLA is worthy of further analysis.

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“…WNT2B is a key inducer of WNT signal transduction, and its abnormal expression is associated with the occurrence and development of human cancers [ 28 , 29 ]. Notably, the role of WNT2B in the progression of DN has been discovered.…”

Section: Discussionmentioning

confidence: 99%

LncRNA HOTAIR facilitates high glucose-induced mesangial cell proliferation, fibrosis and oxidative stress in diabetic nephropathy via regulating miR-147a/WNT2B axis

Wang

Huangfu

et al. 2022

Diabetol Metab Syndr

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Background Long non-coding RNAs (lncRNAs) have been shown to be involved in the regulation of many disease progression. However, the role of lncRNA HOX transcript antisense RNA (HOTAIR) in diabetic nephropathy (DN) remains unclear. Methods High glucose (HG)-induced human mesangial cells (HMC) was used to construct DN cell models in vitro. HMC proliferation was evaluated by CCK8 assay and EDU staining. Protein levels of proliferation markers, fibrosis markers, and wingless-type family member 2B (WNT2B) were measured using western blot analysis. HMC oxidative stress was assessed by determining the levels of oxygen species and malondialdehyde, as well as superoxide dismutase activity. Relative expression levels of lncRNA HOTAIR, microRNA (miR)-147a, and WNT2B were examined using quantitative real-time PCR. The interaction between miR-147a and lncRNA HOTAIR or WNT2B was confirmed by dual-luciferase reporter assay and RIP assay. Results Our data showed that lncRNA HOTAIR knockdown could inhibit the proliferation, fibrosis, and oxidative stress in HG-induced HMC. LncRNA HOTAIR could serve as a sponge of miR-147a. The inhibition effect of lncRNA HOTAIR silencing on the biological functions of HG-induced HMC could be reversed by miR-147a inhibitor. WNT2B was targeted by miR-147a, and its overexpression also overturned the suppressive effect of miR-147a on the proliferation, fibrosis, and oxidative stress of HG-induced HMC. Conclusion In total, our research pointed out that lncRNA HOTAIR could mediate miR-147a/WNT2B axis to promote DN progression.

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MicroRNA‑137 suppresses the proliferation, migration and invasion of cholangiocarcinoma cells by targeting WNT2B

Cited by 10 publications

References 28 publications

PiRNA-63049 inhibits bone formation through Wnt/β-catenin signaling pathway

PiRNA-63049 inhibits bone formation through Wnt/β-catenin signaling pathway

A pan-cancer analysis of the biological function and clinical value of BTLA in tumors

LncRNA HOTAIR facilitates high glucose-induced mesangial cell proliferation, fibrosis and oxidative stress in diabetic nephropathy via regulating miR-147a/WNT2B axis

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