Enhanced mechanical strength and biocompatibility of electrospun polycaprolactone-gelatin scaffold with surface deposited nano-hydroxyapatite

Jaiswal, Akhilesh; Chhabra, Hemlata; Soni, Vivek; Bellare, Jayesh

doi:10.1016/j.msec.2013.02.003

Cited by 67 publications

(48 citation statements)

References 65 publications

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“…As can be seen in Figure , PCL presents 2 sharp peaks at the positions 2θ = 23.5° and 2θ = 21.3°, which confirm PCL crystalline nature . The nHA powder is observed to be highly crystalline, showing the peaks with the intensities of 2θ = 25.95°, 31.85°, 32.45°, 32.95°, and 49.65° . PCL/Gel composite scaffold indicates all PCL characteristic peaks with low intensity (Figure ) that confirms its crystalline nature.…”

Section: Resultsmentioning

confidence: 62%

Incorporation of nanohydroxyapatite and vitamin D3 into electrospun PCL/Gelatin scaffolds: The influence on the physical and chemical properties and cell behavior for bone tissue engineering

Sattary

Khorasani

Rafienia

et al. 2017

Polymers for Advanced Techs

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Scaffold, an essential element of tissue engineering, should provide proper physical and chemical properties and evolve suitable cell behavior for tissue regeneration. Polycaprolactone/Gelatin (PCL/Gel)-based nanocomposite scaffolds containing hydroxyapatite nanoparticles (nHA) and vitamin D3 (Vit D3) were fabricated using the electrospinning method. Structural and mechanical properties of the scaffold were determined by scanning electron microscopy (SEM) and tensile measurement. In this study, smooth and bead-free morphology with a uniform fiber diameter and optimal porosity level with appropriate pore size was observed for PCL/Gel/nHA nanocom-

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Section: Resultsmentioning

confidence: 62%

Incorporation of nanohydroxyapatite and vitamin D3 into electrospun PCL/Gelatin scaffolds: The influence on the physical and chemical properties and cell behavior for bone tissue engineering

Sattary

Khorasani

Rafienia

et al. 2017

Polymers for Advanced Techs

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“…28 The change in Runx-2 gene expression level among samples directly relates to the observed change of OCN expression, since Runx-2 can directly stimulate transcription of OCN-the most abundant nano collagenous protein in bone ECM. [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] The up-regulation of OCN during matrix synthesis and mineralization, 27 is curtail for later stages of osteogenesis and ECM mineral formation. The obtained gene expression profile for MSCs seeded on PCL group was in agreement with other studies.…”

Section: Mscs Proliferation and Differentiation On Electrospun Scaffoldsmentioning

confidence: 99%

Physical and Biological Modification of Polycaprolactone Electrospun Nanofiber by Panax Ginseng Extract for Bone Tissue Engineering Application

2015

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Medicinal plants as a therapeutic agent with osteogenic properties can enhance fracture-healing process. In this study, the osteo-inductive potential of Asian Panax Ginseng root extract within electrospun polycaprolactone (PCL) based nanofibers has been investigated. Scanning electron microscopy images revealed that all nanofibers were highly porous and beadles with average diameter ranging from 250 to 650 nm. The incorporation of ginseng extract improved the physical characteristics (i.e., hydrophilicity) of PCL nanofibers, as well as the mechanical properties. Although ginseng extract increased the degradation rate of pure PCL nanofibers, the porous structure and morphology of fibers did not change significantly after 42 days. It was found that nanofibrous scaffolds containing ginseng extract had higher proliferation (up to ~1.5 fold) compared to the pristine PCL. The qRT-PCR analysis demonstrated the addition of ginseng extract into PCL nanofibers induced significant expression of osteogenic genes (Osteocalcin, Runx-2 and Col-1) in MSCs in a concentration dependent manner. Moreover, higher calcium content, alkaline phosphatase activity and higher mineralization of MSCs were observed compared to the pristine PCL fibers. Our results indicated the promising potential of ginseng extract as an additive to enhance osteo-inductivity, mechanical and physical properties of PCL nanofibers for bone tissue engineering application.

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“…Gelatin (GT)–polycaprolactone (PCL) is a representative polymer blend composed of the natural biopolymer GT and the synthetic polymer PCL. Since the introduction of GT/PCL formulation in an ultrafine fibrous form, this type of fibrous hybrid materials system electrospinnable from using some fluorinated alcohols [such as trifluoroethanol (TFE) and hexafluoroisopropanol] had been widely used as a versatile biomimetic fibrous substrate for engineering diversified tissues, including skin, nerve, muscle, bone, cardiovascular, and cartilage . However, fibers electrospun from TFE solutions show a clear phase separation and inhomogeneity in composition and morphology .…”

Section: Introductionmentioning

confidence: 99%

Effect of inhomogeneity of the electrospun fibrous scaffolds of gelatin/polycaprolactone hybrid on cell proliferation

Feng

Duan

et al. 2014

J Biomedical Materials Res

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In this article, gelatin (GT) and polycaprolactone (PCL) blended with a weight ratio of 50:50 were dissolved in the trifluoroethanol (TFE) or the acetic acid-doped TFE solvent system (0.2% relative to TFE) to prepare fibrous scaffolds of GT/PCL with different compositional and morphological homogeneities (denoted as the group 1 and the group 2 scaffolds) by electrospinning. The morphology and composition of the two groups of fibrous scaffolds were examined by scanning electron microscopy and Fourier transform infrared spectroscopy, respectively. Then, using green fluorescence protein-labeled mouse fibroblasts and HaCaT cells (a human keratinocyte cell line) as the model cells, cell adhesion, morphology, and proliferation were assessed by laser scanning confocal microscopy, scanning electron microscopy, and cell counting kit-8 assay, respectively. The results showed that the morphological and compositional inhomogeneity of the group 1 scaffolds had a remarkable influence on cell adhesion and proliferation. In contrast, there was no significant difference among the group 2 scaffolds because of their good consistency in fiber morphology and composition. Phase separation resultant GT content variance in the group 1 scaffolds is suggested as one of the major causes. This study highlighted the importance of producing morphologically uniform and compositionally homogeneous composite nanofibers while electrospinning natural and synthetic polymer blends.

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Enhanced mechanical strength and biocompatibility of electrospun polycaprolactone-gelatin scaffold with surface deposited nano-hydroxyapatite

Cited by 67 publications

References 65 publications

Incorporation of nanohydroxyapatite and vitamin D3 into electrospun PCL/Gelatin scaffolds: The influence on the physical and chemical properties and cell behavior for bone tissue engineering

Incorporation of nanohydroxyapatite and vitamin D3 into electrospun PCL/Gelatin scaffolds: The influence on the physical and chemical properties and cell behavior for bone tissue engineering

Physical and Biological Modification of Polycaprolactone Electrospun Nanofiber by Panax Ginseng Extract for Bone Tissue Engineering Application

Effect of inhomogeneity of the electrospun fibrous scaffolds of gelatin/polycaprolactone hybrid on cell proliferation

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