Incorporation of nanohydroxyapatite and vitamin D3 into electrospun PCL/Gelatin scaffolds: The influence on the physical and chemical properties and cell behavior for bone tissue engineering

Abstract

Scaffold, an essential element of tissue engineering, should provide proper physical and chemical properties and evolve suitable cell behavior for tissue regeneration. Polycaprolactone/Gelatin (PCL/Gel)-based nanocomposite scaffolds containing hydroxyapatite nanoparticles (nHA) and vitamin D3 (Vit D3) were fabricated using the electrospinning method. Structural and mechanical properties of the scaffold were determined by scanning electron microscopy (SEM) and tensile measurement. In this study, smooth and bead-free morphology with a uniform fiber diameter and optimal porosity level with appropriate pore size was observed for PCL/Gel/nHA nanocom-

Design and fabrication of poly (glycerol sebacate)‐based fibers for neural tissue engineering: Synthesis, electrospinning, and characterization

“…Therefore, the mean fiber diameter of scaffolds significantly decrease by increasing pPGS, which could be related to the decreased viscosity solution and polymer chain tangles by increasing PGS . As the pPGS amount increase, the pore size decrease, which is attributed to the decreased fiber diameter (Table ) …”

“…Various parameters of electrospinning such as the applied voltage, the polymer solution flow rate, temperature, and needle to collector distance have reported to govern the produced fibers quality . Therefore, the above said parameters were fixed for all samples in this study to conduct a better comparison.…”

“…The cell viability and proliferation were evaluated using MTT method 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide after 1, 3, and 7 days of incubation. 23 In brief, after each given day, the medium of each well was removed, and 30 μl of MTT solution was added to each well, to follow by adding 300 μl of RPMI-1640. The medium was removed after 4 hours of incubation, and 300 μl of DMSO was added into each well.…”

“…Cell nucleus was observed by 4′, 6-diamidino-2-phenylindole (DAPI) staining after 7 days of incubation to assess the rate of living cells on each scaffold. 23 Briefly, the cultured cells on scaffolds were fixed by 4% paraformaldehyde for 8 minutes, rinsed with PBS, and then permeabilized in PBS using 0.4% Triton X-100 for 30 minutes. The scaffolds were then rinsed with PBS and stained with 0.5 μg/mL of DAPI for 30 minutes.…”

The use of polymeric meshes for pelvic organ prolapse: Current concepts, challenges, and future perspectives

“…In addition to the possibility of using an electrospinning process to manufacture 3D scaffolds with tailorable fiber design (in terms of both diameter and distribution) as well as mechanical properties, other studies have considered the opportunity to use electrospun meshes as a vehicle to deliver bioactive factors (Hu et al, 2014;Sill & von Recum, 2008). Over the years, a number of drugs including antibiotics, anticancer drugs, as well as vitamins and proteins have been investigated as loading agents for the development of smart electrospun tissue engineering meshes, particularly for skin wound healing and bone tissue engineering applications (Huang, Branford-White, Shen, Yu, & Zhu, 2012;Li, Wang, Williams, et al, 2016;Lu, Jing, Song, & Wang, 2012;Sattary, Khorasani, Rafienia, & Rozve, 2018;Zahedi et al, 2012;Zhang et al, 2006).…”