Enhanced liver metabolism of mutagens and carcinogens in fish living in polluted seawater

Bagnasco, Maria; Camoirano, Anna; Flora, Silvio De; Melodia, F.; Arillo, Attilio

doi:10.1016/0165-7992(91)90119-o

Cited by 64 publications

(21 citation statements)

References 23 publications

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“…Concerning phase II, our results seem in line with previous field studies that failed to observe any alteration in GST and UDPGT activities in fish resident in PAH-contaminated sites (for a review see van der Oost et al, 2003;Martínez-Gómez et al, 2009) although some evidence of inhibition of GST activity has been reported in fish from PAH-contaminated sites (Bagnasco et al, 1991;Tuvikene et al, 1999). The different trends observed for GST and UDPGT activities with respect to other parameters is somehow unexpected as GST and UDPGT are actively involved in cell detoxification and glutathione conjugates in particular are the preferential substrates of MRP2 (Leslie et al, 2005).…”

Section: Hissupporting

confidence: 94%

Transcriptional and post-transcriptional response of drug-metabolizing enzymes to PAHs contamination in red mullet (Mullus barbatus, Linnaeus, 1758): A field study

Torre

Corsi

Nardi

et al. 2010

Marine Environmental Research

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Aim of this study was to evaluate the responsiveness of red mullet (Mullus barbatus) liver detoxification enzymes to PAHs at transcriptional and post-transcriptional levels in the field. Fish were captured in the north-eastern Adriatic Sea, close to an oil refinery. Sixteen PAHs (EPA) were determined in sediments and fish fillets; transcription levels of cyp1a, cyp3a and abcc2 genes and EROD, BROD, B(a)PMO, BFCOD, GST and UDPGT enzymatic activities were measured. Levels of PAHs in sediments reflect the oil pollution gradient of the area, with weak correspondence in fish fillets. cyp1a gene transcription and EROD, B(a)PMO and BFCOD activities were significantly induced in the oil refinery site, and a slight up-regulation of cyp3a and abcc2 was also observed. GST and UDPGT remained unchanged. The present study provides the first data on detoxification responses at transcriptional levels in the liver of red mullet and confirms phase I enzymes as suitable biomarkers of exposure to PAHs in field studies.

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Section: Hissupporting

confidence: 94%

Transcriptional and post-transcriptional response of drug-metabolizing enzymes to PAHs contamination in red mullet (Mullus barbatus, Linnaeus, 1758): A field study

Torre

Corsi

Nardi

et al. 2010

Marine Environmental Research

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“…Fishes exposed to polyaromatic hydrocarbons showed increased superoxide dismutase, catalase and aryl-hydrocarbon hydroxylase activities as well as high GSH, GSSG, and MBA levels (Winston and Di Giulio 199 1). Significant increases in aryl-hydrocarbon hydroxylase, glucose-6-P dehydrogenase, 6-phosphogluconate dehydrogenase and cytochome P-450 were observed in fish living in polluted seawater although decreased glutathione peroxidase and glutathione mnsferase activities were also detected (Bagnasco et al 1991).…”

Section: Parametermentioning

confidence: 98%

Biochemical Indicators of Oxidative Stress in Fish from Polluted Littoral Areas

Rodríguez‐Ariza¹,

Peinado²,

Pueyo³

et al. 1993

Can. J. Fish. Aquat. Sci.

186

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1993. Biochemical indicators of oxidative stress in fish from polluted littoral areas. Can. ). Fish, Aquat. Sci. 50: 2568-2573.Several biochemical parameters have been analyzed in the h e r s of fish from south Atlantic Spanish littoral areas exhibiting different pollution levels. Mugil sp. (grey mullet) from the Huelva Estuary contained higher concentrations of metals, such as Fe and Cu, than those from a reference area (Ciidiz Bay). Similarly, sediments from Huelva showed high concentrations of polyaromatic hydrocarbons, polychlorinated biphenyls, and pesticides. The contaminated animals were subjected to oxidative stress, as indicated by their oxidized glutathione redox status, but shacved low malondialdehyde and Bipohydroperoxide levels. A battery of antioxidant enzymes increased simultaneously in contaminated fishes, including glutathione peroxidase, superoxide disrnutase, catalase, glucose-6-P dehydrogenase, and glutathione reductase. These data suggest that contaminated fish were \veil protected from oxidative stress. Highly significant increases were also observed in cytochrome P-450-associated ethoxyresorufin-0-deethylase activity, and soluble and membrane-bound glutathione-Stransferase, in response to the high levels of organic xensbiotics. We propose that antioxidant enzymes and glutathione-S-transferase might be useful tools for the biomonitoring of environmental pollution.On a 6tudi6 plusieurs pararn&tres dans les foies des psissons provenant des zones avec diff6rents niveaux de pollution au long du littoral Sud-Atlantique espagnol. Ees individus de Mugilsp. provenant de B'estuaire de Huelva contenaient des concentrations en Fe, Cu plus grandes que ceux qui provenaient d'une zone de reference (C5diz). En plus, les sediments de Huelva contenaient concentrations plus grandes de hidrocarbsns polyaromatiques,

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“…Nevertheless, although reduced glutathione peroxidase (25,30) and glutathione reductase (25) activities were found in fish exposed to environmental pollution, both enzymes in general showed induced activities in the liver (7,28,31). These Acute and subchronic exposure to pollutants seems to increase reduced glutathione contents in fish cells (26,(32)(33)(34) while fish submitted to heavy and extensive chronic exposure seem to be unable to maintain high constitutive glutathione and total glutathione levels (11,28; Wilhelm Filho D, Torres MA, Testa CP and Tribess TB, unpublished results; present study). The decrease observed both in reduced and oxidized glutathione concentrations may be attributable to the decrease in total glutathione.…”

Section: Effect Of Pollutionmentioning

confidence: 99%

“…Much evidence indicates that xenobiotics can generate reactive oxygen species, including superoxide anion (O 2 · -), hydrogen peroxide (H 2 O 2 ), hydroxyl radical ( · OH), and singlet oxygen (O 2 1 ), which in turn are responsible for cell and tissue damage associated with different pathologic processes, including mutagenesis and carcinogenesis (5). Recently, oxidative stress and some parameters of fish antioxidant defenses and biotransformation enzymes (i.e., cytochrome P-450 1A1 and glutathione S-transferase, GST) of fish and molluscs have been used as biomarkers of water pollution (6)(7)(8)(9)(10)(11). Although the seasonal interference with the antioxidant defense system has not been well established in these organisms, the data available in the literature (12)(13)(14) suggest a general antioxidant defense enhancement during spring and summer when compared to low temperature periods.…”

Section: Introductionmentioning

confidence: 99%

Influence of season and pollution on the antioxidant defenses of the cichlid fish acará (Geophagus brasiliensis)

Filho

Torres

Tribess

et al. 2001

Braz J Med Biol Res

146

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The livers of Geophagus brasiliensis collected from both a nonpolluted site and a polluted site were analyzed for different antioxidant defenses, O 2 consumption, thiobarbituric acid-reactive substance (TBARS) levels, and histological damage. Compared to controls (116.6 ± 26.1 nmol g -1 ), TBARS levels were enhanced at the polluted site (284.2 ± 25.6 nmol g -1 ), as also was oxygen consumption (86.6 ± 11.3 and 128.5 ± 9.8 µmol O 2 min -1 g -1 , respectively). With respect to enzymatic antioxidants, increased catalase activities (8.7 ± 1.3 and 29.2 ± 2.4 mmol min -1 g -1 , respectively), unchanged superoxide dismutase activities (767.2 ± 113.3 and 563.3 ± 70.2 U g -1 , respectively), and diminished glutathione S-transferase activities (29.0 ± 3.2 and 14.9 ± 3.2 µmol min -1 g -1 , respectively) were detected. Reduced glutathione (1.91 ± 0.17 and 1.37 ± 0.25 mM, respectively), oxidized glutathione (1.50 ± 0.20 and 0.73 ± 0.17 mM, respectively), and total glutathione (3.40 ± 0.26 and 2.07 ± 0.27 mM, respectively) concentrations were also below control values at the polluted site. Nevertheless, the observed ethoxyresorufin-O-deethylase activities (1.34 ± 0.11 and 16.7 ± 0.21 pmol min -1 mg -1 , respectively) showed enhanced values at the polluted site. The main histological damage observed in the hepatocytes from fish collected at the polluted site was characterized by heavy lipid infiltration. Fish collected at the end of spring showed higher O 2 consumption, higher superoxide dismutase and glutathione S-transferase activities, and higher total and oxidized glutathione concentrations compared to the beginning of autumn. No seasonal changes were observed in catalase activities, glutathione or TBARS levels. Fish chronically exposed to relatively high pollution levels seem to be unable to set up adequate antioxidant defenses, probably due to severe injury to their hepatocytes. The higher antioxidant defenses found at the end of spring are probably related to the enhanced activities during high temperature periods in thermoconforming organisms.

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Enhanced liver metabolism of mutagens and carcinogens in fish living in polluted seawater

Cited by 64 publications

References 23 publications

Transcriptional and post-transcriptional response of drug-metabolizing enzymes to PAHs contamination in red mullet (Mullus barbatus, Linnaeus, 1758): A field study

Transcriptional and post-transcriptional response of drug-metabolizing enzymes to PAHs contamination in red mullet (Mullus barbatus, Linnaeus, 1758): A field study

Biochemical Indicators of Oxidative Stress in Fish from Polluted Littoral Areas

Influence of season and pollution on the antioxidant defenses of the cichlid fish acará (Geophagus brasiliensis)

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