Biochemical Indicators of Oxidative Stress in Fish from Polluted Littoral Areas

Abstract

1993. Biochemical indicators of oxidative stress in fish from polluted littoral areas. Can. ). Fish, Aquat. Sci. 50: 2568-2573.Several biochemical parameters have been analyzed in the h e r s of fish from south Atlantic Spanish littoral areas exhibiting different pollution levels. Mugil sp. (grey mullet) from the Huelva Estuary contained higher concentrations of metals, such as Fe and Cu, than those from a reference area (Ciidiz Bay). Similarly, sediments from Huelva showed high concentrations of polyaromatic hydrocarbons, polychlorinated biphenyls, and pesticides. The contaminated animals were subjected to oxidative stress, as indicated by their oxidized glutathione redox status, but shacved low malondialdehyde and Bipohydroperoxide levels. A battery of antioxidant enzymes increased simultaneously in contaminated fishes, including glutathione peroxidase, superoxide disrnutase, catalase, glucose-6-P dehydrogenase, and glutathione reductase. These data suggest that contaminated fish were \veil protected from oxidative stress. Highly significant increases were also observed in cytochrome P-450-associated ethoxyresorufin-0-deethylase activity, and soluble and membrane-bound glutathione-Stransferase, in response to the high levels of organic xensbiotics. We propose that antioxidant enzymes and glutathione-S-transferase might be useful tools for the biomonitoring of environmental pollution.On a 6tudi6 plusieurs pararn&tres dans les foies des psissons provenant des zones avec diff6rents niveaux de pollution au long du littoral Sud-Atlantique espagnol. Ees individus de Mugilsp. provenant de B'estuaire de Huelva contenaient des concentrations en Fe, Cu plus grandes que ceux qui provenaient d'une zone de reference (C5diz). En plus, les sediments de Huelva contenaient concentrations plus grandes de hidrocarbsns polyaromatiques,

Influence of Dioxin and Metal-Contaminated Sediment on Phase I and II Biotransformation Enzymes in Silver Crucian Carp

“…In contrast to EROD activity, the usefulness of the phase II enzyme GST in monitoring aquatic pollution has not been well established (Rodriguez-Ariza et al, 1993). In this study, only a slight (1.4-fold) but significant increase in GST was noted in crucian carp after 4 weeks of exposure to sediments containing with dioxin-like compounds and heavy metals (Table 5).…”

“…However, other studies have demonstrated significant induction (Leaver et al, 1992). Rodriguez-Ariza et al (1993) reported that soluble GST activity increased 1.6-fold, and that of the microsome-associated isoenzyme, 11-fold, in contaminated fish. Oxidative lesions have recently been related to liver tumor formation in fish from polluted environments (Malins and Haimanot, 1991).…”

Polluted water exacerbates Barbus callensis oocyte oxidative status

“…In contrast, previous studies on grey mullet and sardine, 16 Khebbache Wafia et al Sardina pilachardus (Walbaum) report higher catalase and superoxide dismutase activities in fish from polluted areas (Rodriguez-Ariza et al 1993, Peters et al 1994. Other studies have shown that some metal pollutants result in oxidative damage in aquatic organisms (Pretto et al 2010).…”

Biochemical Markers of Oxidative Stress in Brain of Zebrafish Danio rerio Exposed to Different Heavy Metals Lead and Cobalt

“…On the other-hand, the enzymatic antioxidants such as superoxide dismutase, GSH have been shown to be sensitive indicators of increased oxidative stress in Mugil sp. obtained from polluted area containing high concentration of pollutants [53] . Cysteine is the limiting factor of GSH synthesis in cells and reduced glutathione has sulfide functional groups that can capture unpaired electrons and thus is capable of removing harmful free radicals [54] .…”