Enhanced in vitro multiplication of Nothapodytes nimmoniana Graham using semisolid and liquid cultures and estimation of camptothecin in the regenerated plants

Dandin, Vijayalaxmi S.; Murthy, Hosakatte Niranjana

doi:10.1007/s11738-012-0934-x

Cited by 47 publications

(11 citation statements)

References 14 publications

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“…For example, the content of bacoside A in Bacopa monnieri shoot cultures was 3‐fold higher than in field grown plants . Similarly, shoot cultures of Nothapodytes nimmoniana contained several fold higher camptothecin amounts compared to the parent plant .…”

Section: Resultsmentioning

confidence: 96%

Enhanced production of the pharmaceutically important polyphenolic compounds in Vitex agnus castus L. shoot cultures by precursor feeding strategy

Skrzypczak-Pietraszek

Piska

Pietraszek

2018

Engineering in Life Sciences

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Agitated Vitex agnus castus L. shoot cultures were established to analyse the content of selected pharmaceutically important flavonoids and phenolic acids. Two variants (selected from nine ones) of MS medium were prepared: A (BAP 1 mg/L; NAA 0.5 mg/L; GA3 0.25 mg/L) and B (BAP 2 mg/L; NAA 0.5 mg/L). The biomass was harvested after 1, 2, 3,4, 5 and 6 weeks. Four‐week cultures (variant A) were selected to perform the precursor feeding experiment. The L‐phenylalanine dose of 1.6 g/L appears to be the most advantageous. Compared to the control cultures, the content of the individual compounds increased in a range from 1.4 to 17.3‐fold (e.g. p‐coumaric acid – 17.3 fold; casticin – 4.8‐fold). The biomass from in vitro cultures is richer in neochlorogenic acid (16‐fold), p‐coumaric acid (5.3‐fold), rutin (2.8‐fold), caffeic acid (1.5‐fold) and cinaroside (1.5‐fold) than the leaves of its parent greenhouse‐cultivated plants. Extracts contained 30 mg/100 g DW of casticin, but after the hydrolysis its amount increased up to 200 mg/100 g DW and twice exceeded the content in the greenhouse leaves. The results indicate that V. agnus castus agitated shoot cultures might be considered as a potential biotechnological source of some pharmaceutically important compounds, especially casticin, rutin, neochlorogenic and p‐coumaric acids.

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Section: Resultsmentioning

confidence: 96%

Enhanced production of the pharmaceutically important polyphenolic compounds in Vitex agnus castus L. shoot cultures by precursor feeding strategy

Skrzypczak-Pietraszek

Piska

Pietraszek

2018

Engineering in Life Sciences

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“…7.5 lM 2-iP showed highest regeneration frequency (83.3 %) with highest number of shoots (10.16 ± 2.24; Table 1). Effect of different cytokinins on multiple shoot regeneration from callus cultures was previously reported in Jatropha curcas (Maharana et al 2012) and Notahapodytes nimmoniana (Dandin and Murthy 2012a). BAP has been described as a beneficial growth regulator for induction of shoots in other Artemisa species (Sujatha and Ranjitha Kumari 2007; Umer Sharief and Jagadish Chandra 1991).…”

Section: Induction Of Multiple Shoots From Callusmentioning

confidence: 85%

Efficient in vitro propagation of Artemisia nilagirica var. nilagirica (Indian wormwood) and assessment of genetic fidelity of micropropagated plants

Shinde

Sebastian

Jain

et al. 2016

Physiol Mol Biol Plants

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A reliable protocol has been established for in vitro propagation of Artemisia nilagirica var. nilagirica (Indian wormwood), a valuable medicinal plant from India. A highly proliferating organogenic callus was obtained on Murashige and Skoog (MS) medium supplemented with 2.5 lM IAA when nodal explants were cultured on MS medium supplemented with various growth regulators. Further, highest regeneration frequency (83.3 %) of adventitious shoots was observed, when the callus was subcultured on MS medium supplemented with 6-benzylaminopurine (BAP; 2.5 lM) along with 7.5 lM 2-isopentenyl adenine (2-iP). An optimal of 10.16 ± 2.24 shoots were regenerated on medium supplemented with 2.5 lM BAP ? 7.5 lM 2-iP. Quarter strength MS medium supplemented with 10 lM IBA was effective for rooting of the shoots. Ex-vitro plants were normal and were established successfully. Cytological and molecular marker studies showed that regenerated plants showed genetic stability in micro-propagated plants.

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“…The multiplication of shoots in liquid media has several advantages, such as fast rate of multiplication, lack of impurities from agar, dilution of exudates from the explants, and uniform dispersal and better availability of nutrients and growth regulators (Lilia et al, 2002;Ascough and Fennell, 2004;Kalpana et al, 2009;Shekhawat et al, 2014). The liquid medium has been used successfully in various plants systems, especially for micropropagation of tree species (Dandin and Murthy, 2012;Rathore et al, 2014Rathore et al, , 2015. Morphological deformity and deterioration of cultures were not observed in this study; therefore liquid media system could be used in continuous multiplication of shoots of M. citrifolia.…”

Section: Tablementioning

confidence: 99%

Enhanced micropropagation protocol of Morinda citrifolia L. through nodal explants

Shekhawat¹,

Kannan²,

Manokari³

et al. 2015

Journal of Applied Research on Medicinal and Aromatic Plants

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Enhanced in vitro multiplication of Nothapodytes nimmoniana Graham using semisolid and liquid cultures and estimation of camptothecin in the regenerated plants

Cited by 47 publications

References 14 publications

Enhanced production of the pharmaceutically important polyphenolic compounds in Vitex agnus castus L. shoot cultures by precursor feeding strategy

Enhanced production of the pharmaceutically important polyphenolic compounds in Vitex agnus castus L. shoot cultures by precursor feeding strategy

Efficient in vitro propagation of Artemisia nilagirica var. nilagirica (Indian wormwood) and assessment of genetic fidelity of micropropagated plants

Enhanced micropropagation protocol of Morinda citrifolia L. through nodal explants

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