Efficient in vitro propagation of Artemisia nilagirica var. nilagirica (Indian wormwood) and assessment of genetic fidelity of micropropagated plants

Shinde, Smita; Sebastian, Joseph Kadanthottu; Jain, Jyothi Ramesh; Hanamanthagouda, Manohar Shirugumbi; Murthy, Hosakatte Niranjana

doi:10.1007/s12298-016-0379-6

Cited by 36 publications

(17 citation statements)

References 26 publications

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“…Micropropagated plants are cultured from explants, established in closed containers with nutrient media and hormone supply that result in high levels of genetic and phenotypic uniformity. Usually, micropropagation requires fully equipped labs, relatively high amount of reagents, well trained people and many labor hours which results in high plant costs (Shinde et al, 2016). Several studies related to developing micropropagation strategies for arrow cane in order to produce massive plant material for planting commercial crops have been reported Suarez, 2009, Suarez et al, 2009;Rivera et al, 2009;Suarez et al, 2017); however, micropropagated plants results in higher costs than planting material from natural populations.…”

Section: Introductionmentioning

confidence: 99%

In vitro arrow cane (Gynerium sagitatum Aubl.) multiplication in double phase medium

Lopez

Suarez

2018

rcia

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Arrow cane (Gynerium sagitatum Aubl.) is a Poaceae species used as fiber source to make traditional and valuable handmade craftsmanship by indigenous communities in Northern Colombia. Since no commercial crops are established fiber needs are taken from natural plant populations affecting ecosystem. A micropropagation protocol to clonally multiply large quantities of arrow cane plant material for planting commercial crops has been developed; however, micropropagated plants are costly compared to naturally extracted plant material. To reduce micropropagated plants costs, in the present research a double phase medium formulation along with continuous shoot culture with no periodic transfers to fresh medium was compared to semisolid medium system with subculture every four weeks with respect to multiplication rate and costs of micropropagated plants. The results showed that continuous culture of explants with double phase medium and no periodic transfers resulted in higher multiplication rates and larger shoots compared to shoots cultured using the conventionalsemisolid medium system and transfer to fresh medium every four weeks. Plants from both, semisolid and double phase culture system, fully adapted and recovered when transferred to ex vitro conditions. The cost analysis showed that double phase cultured shoots are ≥20% less expensive.

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Section: Introductionmentioning

confidence: 99%

In vitro arrow cane (Gynerium sagitatum Aubl.) multiplication in double phase medium

Lopez

Suarez

2018

rcia

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“…It was also proclaimed that with increase the strength of SH medium decreases the regenerated root number as well their root growth (Thwe et al, 2013). Quarter strength MS medium along with 10 µM IBA was effective for rooting of the shoots (Shinde et al, 2016) of Artemisia nilagirica. MS medium promotes the highest growth of Cladanthus mixtus with an average of 2.75±0.12 cm shoot length and 2.60±0.29 shoots per explants and the mean number of roots achieved 3.33±0.17 root per explants with a length of 2.42±0.16 cm (Harras and Lamarti, 2014).…”

Section: Discussionmentioning

confidence: 99%

Enhancement of <i>in vitro</i> Rooting through Growth Media, Gelling Agents and Activated Charcoal in <i>Lycium chinense</i>

Kim¹,

Park²

2017

OnLine Journal of Biological Sciences

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Lycium chinense, a traditional Chinese herbal medicine is well known for its medicinal value and composition for centuries in Asia. Plant tissue culture technique has proven as a quick and sustainable ways to regenerate various plants species those are valuable in terms of medicinal as well as ornamental. Here, we investigated the in vitro root regeneration and root growth of L. chinense in response of different media, gelling agents and activated charcoal. The growth media Schenk and Hildebrandt medium (SH medium) performed the best for being the highest number of roots in each (3.50) and also for the longest root length (33.80 mm) exhibiting 20% and 18% higher roots/explant and root growth, respectively than that of the lowest roots/explant and root growth containg medium of MS. The highest number of roots (3.90) in each explant was observed when phytagar at 6 g L −1 has been used in the culture media producing a 26% higher number of roots in each explant. Phytagar at the lowest concentration (5 g L −1 ) used in this study produced the longest root length (35.10 mm) exhibiting 20% higher root length than that of the highest concentration (9 g L −1 ) of phytagar and after that with further increase the concentration of Phytagar the growth of the root length has been decreased. Here concentration of Gelrite at 3 g L −1 responded positively for getting the highest number of roots (4.00) in each explants and the longest root length (35.2 mm). Activated charcoal at 1 g L −1 produced the highest roots (4.10) in each explant and also enhanced to produce the longest (45.40 mm) root length these findings could contributed to enhance rooting ability in any crops especially some medical and ornamental cops and even could provide useful information for future industrial-scale root production of L. chinense.

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“…In vitro manipulation of different Artemisia species such as A. annua [ 20 , 21 , 22 , 23 ], A. sieberi [ 24 ], A. vulgaris [ 25 , 26 ], A. japonica [ 27 ], A. nilagirica var. nilagirica [ 28 ], A. absinthium [ 29 , 30 ], A. abrotanum [ 31 ], A. amygdalina [ 32 ], A. carvifolia [ 33 ], A. aucheri [ 34 ], A. scoparia [ 35 ], A. judaica [ 36 ], A. absinthium [ 37 ], A. chamaemelifolia [ 38 ] and A. pallens [ 39 , 40 ] has been attempted for various purposes. Some investigations were tried to increase the number of glandular trichomes as the organ responsible for accumulation of artemisinin in A .…”

Section: Production Of the Sesquiterpene Artemisinin In mentioning

confidence: 99%

Production of Plant Secondary Metabolites: Examples, Tips and Suggestions for Biotechnologists

Guerriero

Berni

Muñoz-Sánchez

et al. 2018

Genes

261

138

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Plants are sessile organisms and, in order to defend themselves against exogenous (a)biotic constraints, they synthesize an array of secondary metabolites which have important physiological and ecological effects. Plant secondary metabolites can be classified into four major classes: terpenoids, phenolic compounds, alkaloids and sulphur-containing compounds. These phytochemicals can be antimicrobial, act as attractants/repellents, or as deterrents against herbivores. The synthesis of such a rich variety of phytochemicals is also observed in undifferentiated plant cells under laboratory conditions and can be further induced with elicitors or by feeding precursors. In this review, we discuss the recent literature on the production of representatives of three plant secondary metabolite classes: artemisinin (a sesquiterpene), lignans (phenolic compounds) and caffeine (an alkaloid). Their respective production in well-known plants, i.e., Artemisia, Coffea arabica L., as well as neglected species, like the fibre-producing plant Urtica dioica L., will be surveyed. The production of artemisinin and caffeine in heterologous hosts will also be discussed. Additionally, metabolic engineering strategies to increase the bioactivity and stability of plant secondary metabolites will be surveyed, by focusing on glycosyltransferases (GTs). We end our review by proposing strategies to enhance the production of plant secondary metabolites in cell cultures by inducing cell wall modifications with chemicals/drugs, or with altered concentrations of the micronutrient boron and the quasi-essential element silicon.

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Efficient in vitro propagation of Artemisia nilagirica var. nilagirica (Indian wormwood) and assessment of genetic fidelity of micropropagated plants

Cited by 36 publications

References 26 publications

In vitro arrow cane (Gynerium sagitatum Aubl.) multiplication in double phase medium

In vitro arrow cane (Gynerium sagitatum Aubl.) multiplication in double phase medium

Enhancement of <i>in vitro</i> Rooting through Growth Media, Gelling Agents and Activated Charcoal in <i>Lycium chinense</i>

Production of Plant Secondary Metabolites: Examples, Tips and Suggestions for Biotechnologists

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