Enhanced healing of cartilaginous injuries by glucosamine hydrochloride

Tamai, Yoshin; Miyatake, K; Okamoto, Yoshiharu; Takamori, Yoshimori; Sakamoto, Hiroshi; Minami, Saburo

doi:10.1016/s0144-8617(01)00281-8

Cited by 24 publications

(11 citation statements)

References 8 publications

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“…This compound is an intermediate substrate involved in the biosynthesis of proteoglycans and glycosaminoglycan that are found in gastrointestinal mucosal membranes, the articular cartilage matrix and synovial fluid [2,3]. It has been suggested that GL from exogenous sources stimulates the production of proteoglycans and incorporates into the metabolic pathway for glycosaminoglycan synthesis in the articular cartilage, thus inhibiting its degradation [4] and indeed can rebuild damaged cartilage [5].…”

Section: Introductionmentioning

confidence: 99%

An optimized reverse-phase high performance liquid chromatographic method for evaluating percutaneous absorption of glucosamine hydrochloride

Tekko

Bonner

Williams

2006

Journal of Pharmaceutical and Biomedical Analysis

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Section: Introductionmentioning

confidence: 99%

An optimized reverse-phase high performance liquid chromatographic method for evaluating percutaneous absorption of glucosamine hydrochloride

Tekko

Bonner

Williams

2006

Journal of Pharmaceutical and Biomedical Analysis

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“…We previously reported effective regeneration of experimentally damaged cartilaginous tissue by oral administration of glucosamine and collagen peptide in a rabbit model 4–6 …”

mentioning

confidence: 99%

Enthesiopathy with an abductor pollicis longus dysfunction in a Rough Collie dog

Okamura¹,

Okamoto²,

T³

et al. 2007

Aust Veterinary J

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APLAbductor pollicis longus E nthesiopathy is well documented as a distinctive pathological feature of spondyloarthropathy in human medicine, 1 however, few reports have been published in the veterinary literature 2,3 and most of these have been limited to the racing Greyhound. In Japan, dog racing is not a recognised sport, and this disease has not been described. Here we present a Rough Collie dog with thoracic limb lameness. Radiographic examination indicated enthesiopathy of the right short radial collateral ligaments accompanying abductor pollicis longus (APL) dysfunction.A 10-year-old female Collie dog weighing 32.6 kg and with weight-bearing lameness of the right foreleg was brought to the Tottori University Veterinary Teaching Hospital (TUVTH) in Japan. The dog had been exercised, with the owner riding a bicycle and the dog running alongside, for 2 km, twice daily, from a young age. From the age of 9 years, the dog showed mild lameness after this exercise that became severe after 30 min rest. The lameness had always resolved by the following morning. These signs continued for almost a year, and then became more severe over a period of a month. Upon admission, the dog showed slight weight-bearing lameness of the right front leg at the walk. On the dorso-medial side of the distal end of the radius, a firm nodular swelling and mild skin heat was observed. No pain was evident upon deep palpation, or on rotation or flexion of the carpal joint.Radiographs showed an osteophyte in the radius (Figure 1) and calcification on the medial surfaces of the radius and the carpal bones ( Figure 2). There were no inflammatory changes evident in the carpal joint. The density of the right flexor carpi radialis was less than that of the left.The owner declined surgical intervention, and treated the dog at home with 1 g/day each of glucosamine and fish collagen peptide orally for 2 weeks. There was no improvement in the dog's lameness, and identical radiographic findings were present in the right radius at the second examination.Osteophyte resection was performed under general anaesthesia using propofol and midazoram for induction, and inhalation anaesthesia with nitrous oxide, oxygen and isoflurane for maintenance. A skin incision was made over the osteophyte with a 2 cm margin at both the proximal and distal ends. Following subcutaneous tissue dissection, the osteophyte was easily visualised. The APL was trapped by the elongated osteophyte and the distal part of the APL was buried in firm connective tissue. Blunt dissection of the connective tissue revealed the distal APL, which was kinked at the osteophyte elongation. The osteophyte was completely removed using Rongeur forceps. Complete resection of the osteophyte was confirmed radiographically (Figure 3). Figure 1. Dorso-palmar radiographic view of the right carpal joint of a 10-year-old Rough Collie that had signs of lameness after exercise. There is soft tissue swelling (large white arrow) and an osteophyte with characteristic elongation (arrow head) and calcification (s...

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“…In the Dglucosamine group, trabecular bones physically damaged by surgery were quickly absorbed and disappeared compared with the control This phenomenon gave rise to some suspicion that activation of osteoclasts may have occurred in the glucosamine group as opposed to the control. [5] From the present results, it is important that for the repair of trabecular bone, the supplement is not only D-glucosamine but also protein of a collagen component. It is suspected that the mechanism for repair is by activation of osteoblasts by these supplements.…”

Section: Results Of Image Analysis On Safranin O-and Alcian Blue-staimentioning

confidence: 62%

“…[4] We have reported an accelerated healing of experimental cartilage damage by glucosamine. [5] Full Paper: We investigated the synergistic wound-healing effects of D-glucosamine (GlcN) and collagen peptide (M w : 10 000 Daltons soluble collagen (S-Co)) on injured cartilage. A injured cartilage model in rabbit was surgically created.…”

Section: Introductionmentioning

confidence: 99%

Synergistic Effects of D‐Glucosamine and Collagen Peptides on Healing Experimental Cartilage Injury

Hashida

Miyatake

Okamoto

et al. 2003

Macromolecular Bioscience

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We investigated the synergistic wound‐healing effects of D‐glucosamine (GlcN) and collagen peptide ($\overline M _{\rm w}$: 10 000 Daltons soluble collagen (S‐Co)) on injured cartilage. A injured cartilage model in rabbit was surgically created. Under general anesthesia, three holes at articular cartilage of the medial trochlear (one hole) and trochlear sulcus (two holes) of a distal femur were created using a high‐speed orthopedic drill. After surgery, rabbits were orally administered with collagen peptide (S‐Co group), GlcN (G group), or both (CoG group) for 2 or 3 weeks. In addition, glycine (Gly group) was administered as a control for the S‐Co group in three rabbits. Control rabbits were provided with water ad lib. After 2 weeks, the holes were filled by fibroblasts and chondroblasts in the treatment group, whereas in the control group, the holes were not filled completely unlike for the treatment group. Even three weeks later, only a small change was seen in the control group. In the group receiving collagen and GlcN, the holes were completely filled by proliferating chondroblasts, remodeling of bony trabeculae was also observed. Image analysis of Safranin O‐ and Alcian blue‐stained specimens showed increased production of proteoglycans and glycosaminoglycans, respectively, not only in the injured site, but also in the normal cartilage in the treated groups. The most enhanced healing of injury and increase in Safranin O and Alcian blue positive materials was observed in the CoG group. As a result, simultaneous administration of collagen and GlcN gave a fairly enhanced healing on restoration of cartilage injuries.Artificial cartilage injury at the stifle joint of a rabbit. a: proximal hole in trochlear sulcus; b: distal hole in trochlear sulcus; c: medial hole in medial trochlear ridge.magnified imageArtificial cartilage injury at the stifle joint of a rabbit. a: proximal hole in trochlear sulcus; b: distal hole in trochlear sulcus; c: medial hole in medial trochlear ridge.

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Enhanced healing of cartilaginous injuries by glucosamine hydrochloride

Cited by 24 publications

References 8 publications

An optimized reverse-phase high performance liquid chromatographic method for evaluating percutaneous absorption of glucosamine hydrochloride

An optimized reverse-phase high performance liquid chromatographic method for evaluating percutaneous absorption of glucosamine hydrochloride

Enthesiopathy with an abductor pollicis longus dysfunction in a Rough Collie dog

Synergistic Effects of D‐Glucosamine and Collagen Peptides on Healing Experimental Cartilage Injury

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