An optimized reverse-phase high performance liquid chromatographic method for evaluating percutaneous absorption of glucosamine hydrochloride

Tekko, Ismaiel A.; Bonner, Michael; Williams, Adrian C.

doi:10.1016/j.jpba.2005.11.044

Cited by 22 publications

(25 citation statements)

References 15 publications

(16 reference statements)

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“…The half-life estimated from glucosamine disappearance curves in our lean subjects was ϳ150 min, consistent with previous studies in humans (28,41). Glucosamine is a highly hydrophilic, fully ionized compound predominantly distributed in aqueous space (42). Given the aqueous distribution and relatively short half-life of glucosamine, it is unlikely that dosing three times daily would result in an increase in steady-state levels of plasma glucosamine.…”

Section: Discussionsupporting

confidence: 89%

Oral Glucosamine for 6 Weeks at Standard Doses Does Not Cause or Worsen Insulin Resistance or Endothelial Dysfunction in Lean or Obese Subjects

et al. 2006

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Glucosamine is a popular nutritional supplement used to treat osteoarthritis. Intravenous administration of glucosamine causes insulin resistance and endothelial dysfunction. However, rigorous clinical studies evaluating the safety of oral glucosamine with respect to metabolic and cardiovascular pathophysiology are lacking. Therefore, we conducted a randomized, placebo-controlled, double-blind, crossover trial of oral glucosamine at standard doses (500 mg p.o. t.i.d.) in lean (n ‫؍‬ 20) and obese (n ‫؍‬ 20) subjects. Glucosamine or placebo treatment for 6 weeks was followed by a 1-week washout and crossover to the other arm. At baseline, and after each treatment period, insulin sensitivity was assessed by hyperinsulinemic-isoglycemic glucose clamp (SI Clamp ) and endothelial function evaluated by brachial artery blood flow (BAF; Doppler ultrasound) and forearm skeletal muscle microvascular recruitment (ultrasound with microbubble contrast) before and during steady-state hyperinsulinemia. Plasma glucosamine pharmacokinetics after oral dosing were determined in each subject using a high-performance liquid chromatography method. As expected, at baseline, obese subjects had insulin resistance and endothelial dysfunction when compared with lean subjects (SI Clamp

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Section: Discussionsupporting

confidence: 89%

Oral Glucosamine for 6 Weeks at Standard Doses Does Not Cause or Worsen Insulin Resistance or Endothelial Dysfunction in Lean or Obese Subjects

et al. 2006

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“…The reaction produces a PITC-glucosamine ( fig. 1c) which according to the work of Tekko and colleagues gave maximum at 245 nm [10], compared to our work which is 240 nm ( fig. 4), whereas the analytical work of Shen for PITC-glucosamine was set at 254 nm [11].…”

Section: Fig 2: Potential Energy Against Time During Geometry Optimicontrasting

confidence: 55%

Prediction of Log P and Spectrum of Quercetine, Glucosamine, and Andrographolide and Its Correlation With Laboratory Analysis

Megantara

Mutakin

Levita

2016

Int J Pharm Pharm Sci

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Objective: This study was aimed to confirm the result of computational prediction of log P and spectrum 13 Methods: Quercetine, glucosamine and andrographolide, were downloaded from ChemSpider and were geometry optimised. Log P and spectrum were calculated and predicted and the data obtained were compared with laboratory results. The correlation was calculated by employing mean absolute deviation (MAD), mean square error (MSE), mean forecast error (MFE), and mean absolute percentage error (MAPE) parameters.C-NMR) of quercetin, glucosamine and andrographolide with laboratory analysis.Results: The smallest energy value of geometry optimisation was provided by ab initio method. Log P prediction showed good accuracy, with r-value 0.995 and p-value 0.05 respectively. The error parameters were: MAD 0.19; MSE 0.06; MFE 0.16, and MAPE 8.62%, respectively. Prediction of λ maximum by ab initio, semiempirical, and molecular mechanics were respectively: MAD 2. 67, 6.67, and 28.67; MSE 8.67, 45.33, and 830; MFE 2.67, 6.67, and 28.67; and MAPE 1.10%, 2.79%, and 11.99%; r-value 0.997, 0.997, and 0.979; and p-value 0.044, 0.043, and 0.129. 1 H-NMR and 13Conclusion: There is a positive correlation between computational ab initio calculation method with experimental results in predicting log P and spectrum of quercetine, glucosamine, and andrographolide.C-NMR spectra prediction were: MAD 0.73 and 1.58; MSE 1.15 and 7.41; MFE 0.27 and 0.69; MAPE 18.35% and 2.68%; r-value 0.942 and 0.986; and p-value 0.001 and 0.001.

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“…1) is an intermediate substrate involved in the biosynthesis of proteoglycans and glycosaminoglycan that are found in the synovial fluid, articular cartilage matrix and gastrointestinal mucosal membranes. Glucosamine from exogenous sources can lead to rebuild damaged cartilage by stimulating the production of proteoglycans and being incorporated into the metabolic pathway for glycosaminoglycan synthesis occurred in the articular cartilage (Tekko et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Pharmacokinetic studies in rats showed that orally administered glucosamine undergoes extensive hepatic first-pass metabolism resulting in a relatively low bioavailability of about 19% (Tekko et al, 2006). Due to this extensive first-pass metabolism and thereby rapid elimination of glucosamine from the systemic circulation, transdermal delivery appears to be an attractive and alternative means against oral …”

Section: Introductionmentioning

confidence: 99%

Identification and assessment of permeability enhancing vehicles for transdermal delivery of glucosamine hydrochloride

et al. 2010

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As an initial step to develop the transdermal delivery system of glucosamine hydrochloride (GL-HCl), the permeation study across the rat skin in vitro was performed to identify the most efficient vehicle with regard to the ability to deliver GL-HCl transdermally. The GL-HCl formulations such as o/w cream, liposome suspension, liposomal gel, and liquid crystalline vehicles were prepared and compared for transdermal flux of GL-HCl. The liquid crystalline vehicles were more effective in increasing the skin permeation of GL-HCl than o/w cream and liposomal vehicles. Of the liquid crystalline vehicles tested, the permeation enhancing ability of the cubic phase was greater than that of the hexagonal phase when the nanoparticle dispersion was used. The skin permeation enhancing ability of the cubic nanoparticles for GL-HCl was further increased by employing both oleic acid and polyethylene glycol 200. Therefore, the cubic liquid crystalline nanodispersion containing oleic acid and PEG 200 can provide a possibility of clinical application of transdermal GL-HCl.

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An optimized reverse-phase high performance liquid chromatographic method for evaluating percutaneous absorption of glucosamine hydrochloride

Cited by 22 publications

References 15 publications

Oral Glucosamine for 6 Weeks at Standard Doses Does Not Cause or Worsen Insulin Resistance or Endothelial Dysfunction in Lean or Obese Subjects

Oral Glucosamine for 6 Weeks at Standard Doses Does Not Cause or Worsen Insulin Resistance or Endothelial Dysfunction in Lean or Obese Subjects

Prediction of Log P and Spectrum of Quercetine, Glucosamine, and Andrographolide and Its Correlation With Laboratory Analysis

Identification and assessment of permeability enhancing vehicles for transdermal delivery of glucosamine hydrochloride

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