Enhanced healing activity of burn wound infection by a dextran-HA hydrogel enriched with sanguinarine

Abstract

Burn wounds are associated with a series of risks, such as infection and pathologic scar tissue formation, which significantly delay wound healing and lead to complications. In this study, we successfully fabricated a dextran-hyaluronic acid (Dex-HA) hydrogel enriched with sanguinarine (SA) incorporated into gelatin microspheres (GMs), which had high porosity, good swelling ratio, enhanced NIH-3T3 fibroblast cell proliferation, and sustained SA release profile. The in vitro degradation results indicate that the SA/GMs/Dex-HA hydrogel can be degraded. The in vitro antibacterial tests showed that the SA/GMs/Dex-HA hydrogel can inhibit methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli (E. coli). We evaluated the wound-healing effects and antibacterial properties of SA/GMs/Dex-HA hydrogels in a rat full-thickness burn infection model. The hematoxylin-eosin (H&E) and Masson's trichrome staining results of the SA/GMs/Dex-HA hydrogel showed that it improved re-epithelialization and enhanced extracellular matrix remodeling, and immunohistochemistry results showed that the expression of TGF-β1 and TNF-α was decreased, while the TGF-β3 expression was increased. Our findings suggest that the SA/GMs/Dex-HA hydrogel provides a potential way for infected burn treatment with high-quality and efficient scar inhibition.

Hybrid cellulose nanocrystal/alginate/gelatin scaffold with improved mechanical properties and guided wound healing

“…8,9 With better understanding of the wound repair and management process, numerous biomaterials have been created to mimic the natural environment for skin regeneration, and accelerate cell proliferation and induce extracellular matrix (ECM) reproduction. 10,11 To date, a number of naturally derived polymers, including polysaccharides and proteins have been widely utilized in the eld of tissue engineering scaffolds, due to their excellent hydrophilicity, biodegradability and biocompatibility. [12][13][14] In particular, sodium alginate, a seaweed-derived polysaccharides, is preferred for its gelling property, excellent biocompatibility, and source-abundance.…”

Polysaccharide-Based Formulations for Healing of Skin-Related Wound Infections: Lessons from Animal Models and Clinical Trials

“…Firstly, the in vitro antimicrobial action of the formulation was evaluated against MRSA and E. coli, along with the mechanical properties and drug release pattern. In summary, the in vitro assay demonstrated that the hydrogel promoted a sustainable release of sanguinarine and preserved its antimicrobial activity [73].…”

“…The obtained results showed that SA/GMs/Dex-HA hydrogel favored the healing process of burn lesion infected by MRSA when compared with rat treated with dextran-hyaluronic acid hydrogel or dextran-hyaluronic acid hydrogel incorporated with sanguinarine. In fact, the mice treated by SA/GMs/Dex-HA showed better profiles of re-epithelialization and extracellular matrix remodeling [73]. Furthermore, the treatment down-regulated the amounts of TGF-β1 (transforming growth factor-β1; related with hypertrophic scars and keloids formation due to myofibroblast differentiation [109,110]) and TNF-α; and up-regulated the expression of TGF-β3 (transforming growth factor-β3; inductor of collagen synthesis and fibroblast proliferation [111]).…”

“…Furthermore, the treatment down-regulated the amounts of TGF-β1 (transforming growth factor-β1; related with hypertrophic scars and keloids formation due to myofibroblast differentiation [109,110]) and TNF-α; and up-regulated the expression of TGF-β3 (transforming growth factor-β3; inductor of collagen synthesis and fibroblast proliferation [111]). The authors did not report data about bacterial load in the tissue [73].…”

Preparation of Antimicrobial Hyaluronic Acid/Quaternized Chitosan Hydrogels for the Promotion of Seawater-Immersion Wound Healing

“…When the hydrogels were submerged in PBS in the presence of hyaluronidase, the weight loss by degradation was significantly higher than that in PBS solution ( Figure 3B). All of the hydrogels degraded by 82-93% after 28 days; this fast degradation rate was related to the β-elimination caused by hyaluronidase (Zhu et al, 2018b). Compared with OHA/HA-ADH, the OHA/HA-ADH/O-HACC and OHA/HA-ADH/N-HACC hydrogels shown a lower weight loss ratio.…”