Enhanced generation of retinal progenitor cells from human retinal pigment epithelial cells induced by amniotic fluid

Sanie-Jahromi, Fatemeh; Ahmadieh, Hamid; Soheili, Zahra‐Soheila; Davari, Maliheh; Ghaderi, Shahla; Kanavi, Mozhgan Rezaei; Samiei, Shahram; Deezagi, Abdolkhalegh; Pakravesh, Jalil; Bagheri, Abouzar

doi:10.1186/1756-0500-5-182

Cited by 20 publications

(10 citation statements)

References 37 publications

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“…hRPE cells were routinely isolated and each sample was assessed by ICC tests for RPE 65 and cytokeratin as specific markers for RPE cells. [ 20 ] hRPE cells were established in the 6 th passage of general hRPE cultures. These cells made sizeable spaces, devoid of cells, between the monolayer of hRPE cells, and gradually invaded unoccupied spaces.…”

Section: Resultsmentioning

confidence: 99%

Behavior of a spontaneously arising human retinal pigment epithelial cell line cultivated on thin alginate film

Najafabadi

Soheili

Ganji

2015

J Ophthalmic Vis Res

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Purpose:A cell line spontaneously derived from human retinal pigment epithelium (hRPE) was cultured on alginate film gelatinized with different concentrations of neurobasal cell culture medium (NCCM) to assess its growth and morphological behavior on this naturally occurring polysaccharide.Methods:Neonatal human globes were used to isolate hRPE cells. They were cultured in Dulbecco's modified Eagle’s-medium-and-Ham’s-F12-medium-(DMEM/F12) supplemented with 10% fetal bovine serum (FBS). Cultures were continuously studied using phase contrast microscopy. After the nineth passage, cells were characterized through immunocytochemical analysis for Oct4, Chx10, and Pax6 and Ki67 markers. In each well of a 6-well microplate, 1 and 2% weight/volume (w/v) alginate in deionized water was added and gelatinized using 1× and 10× NCCM. hRPE cells were cultured at a density of 2 × 105 cells/well in alginate-coated microplates. After 5 days, hRPE colonies were harvested and re-plated on polystyrene substrates. Morphology and growth of hRPE cultures were determined during the next 2 weeks.Results:The first few passages of the cultures were purely hRPE cells that revealed typical morphological features of the pigmented epithelium. They made spaces, devoid of cells, between hRPE cell monolayer and fill in the unoccupied spaces. They grew faster than native RPE cells and rapidly overgrew. Immunocytochemical test revealed that the founded cells expressed Chx10, Pax6, Ki67 and Oct4. The hRPE cells survived unlimitedly on alginate film and formed giant adjoining colonies. After re-plating, hRPE colonies adhered quickly on polystyrene and displayed native hRPE morphological features.Conclusion:Alginate film can support the survival and growth of hRPE cells and induce the cells to re-organize in tissue-like structures.

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Section: Resultsmentioning

confidence: 99%

Behavior of a spontaneously arising human retinal pigment epithelial cell line cultivated on thin alginate film

Najafabadi

Soheili

Ganji

2015

J Ophthalmic Vis Res

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“…The study showed that the longer PMI, the time of neurospheres formation increases ( Carter et al, 2007 ). Other research conducted on the cadavers showed that adding the amniotic fluid preparation to the in vitro RPCs medium significantly increases the generation rate of retinal progenitor cells ( Sanie-Jahromi et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Cadaveric Stem Cells: Their Research Potential and Limitations

Cieśla

Tomsia

2021

Front. Genet.

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In the era of growing interest in stem cells, the availability of donors for transplantation has become a problem. The isolation of embryonic and fetal cells raises ethical controversies, and the number of adult donors is deficient. Stem cells isolated from deceased donors, known as cadaveric stem cells (CaSCs), may alleviate this problem. So far, it was possible to isolate from deceased donors mesenchymal stem cells (MSCs), adipose delivered stem cells (ADSCs), neural stem cells (NSCs), retinal progenitor cells (RPCs), induced pluripotent stem cells (iPSCs), and hematopoietic stem cells (HSCs). Recent studies have shown that it is possible to collect and use CaSCs from cadavers, even these with an extended postmortem interval (PMI) provided proper storage conditions (like cadaver heparinization or liquid nitrogen storage) are maintained. The presented review summarizes the latest research on CaSCs and their current therapeutic applications. It describes the developments in thanatotranscriptome and scaffolding for cadaver cells, summarizes their potential applications in regenerative medicine, and lists their limitations, such as donor’s unknown medical condition in criminal cases, limited differentiation potential, higher risk of carcinogenesis, or changing DNA quality. Finally, the review underlines the need to develop procedures determining the safe CaSCs harvesting and use.

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“…[1][2][3] Through the production of different fibrotic and angiogenic factors in choroidal neovascular membranes, retinal pigment epithelial (RPE) cells contribute to choroidal neovascularization (CNV) and paracrine signaling between choriocapillaris and RPE layer. [4][5][6][7] Hypoxia is an important regulator of cell migration and angiogenesis, especially under pathologic conditions, which perform its action by upregulating hypoxiainducible factor 1 (HIF-1) and vascular endothelial growth factor A (VEGFA). [8][9][10][11] VEGFA mainly regulates angiogenesis, contributing to the migration and proliferation of vascular endothelial cells and tube formation, which increase vascular permeability in angiogenesis.…”

Section: Introductionmentioning

confidence: 99%

Correlation between ELF–PEMF exposure and Human RPE Cell Proliferation, Apoptosis and Gene Expression

Oladnabi

Mishan

Rezaeikanavi

et al. 2021

JOVR

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Purpose: Emerging evidence implies that electromagnetic fields (EMFs) can negatively affect angiogenesis. In this regard, the effects of extremely low frequency pulsed electromagnetic field (ELF–PEMF) exposure on the relative expression level of angiogenic factors involved in the pathogenesis of ocular disorders were evaluated in human retinal pigment epithelial (hRPE) cells in order to investigate a noninvasive therapeutic method for patients with several ocular diseases associated with neovascularization. Methods: After separating hRPE cells from globes, hRPE cells were exposed to 15 mT of ELF–PEMF (120 Hz) at 5, 10, and 15 min for seven days. Cell proliferation and apoptosis of treated cells were evaluated via ELISA assay. Moreover, relative expression changes of HIF-1α, CTGF, VEGFA, MMP-2, cathepsin D, and E2F3 were performed using real-time RT-PCR. Results: ELF–PEMF exposure had no significant effects on the apoptosis and proliferation rate of hRPE cells. Expression level of HIF-1α, CTGF, VEGFA, MMP- 2, cathepsin D, and E2F3 was downregulated following 5 min of ELF–PEMF exposure. Conclusion: As ELF–PEMF showed inhibitory effects on the expression of angiogenic genes in hRPE cells with no cytotoxic or proliferative side effects, it can be introduced as a useful procedure for managing angiogenesis induced by retinal pathogenesis, although more studies with adequate follow-up in animal models are needed.

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Enhanced generation of retinal progenitor cells from human retinal pigment epithelial cells induced by amniotic fluid

Cited by 20 publications

References 37 publications

Behavior of a spontaneously arising human retinal pigment epithelial cell line cultivated on thin alginate film

Behavior of a spontaneously arising human retinal pigment epithelial cell line cultivated on thin alginate film

Cadaveric Stem Cells: Their Research Potential and Limitations

Correlation between ELF–PEMF exposure and Human RPE Cell Proliferation, Apoptosis and Gene Expression

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