Cadaveric Stem Cells: Their Research Potential and Limitations

Cieśla, Julia; Tomsia, Marcin

doi:10.3389/fgene.2021.798161

Cited by 10 publications

(10 citation statements)

References 126 publications

(199 reference statements)

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“…The main research on this topic has been extensively reviewed in Cieśla and Tomsia. 56 As seen from our experience on post-mortem vascular tissues, MSCs from cadaveric tissues retain stemness and differentiation features. Thus, the utilization of cadaveric sources for the isolation of MSCs provides for a promising investigation field to allow MSC-based therapies in terms of safety and effectiveness.…”

Section: Ethical Considerationsmentioning

confidence: 84%

The Characteristics and Survival Potential Under Sub-lethal Stress of Mesenchymal Stromal/Stem Cells Isolated from the Human Vascular Wall

2022

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Mesenchymal Stromal/Stem Cells (MSCs) have been identified in multiple human tissues, including the vascular wall. High proliferative potential, multilineage and immunomodulatory properties make vascular MSCs promising candidates for regenerative medicine. Indeed, their location is strategic for controlling vascular and extra-vascular tissue homeostasis. However, the clinical application of MSCs, and in particular vascular MSCs, is still challenging. Current studies are focused on developing strategies to improve MSC therapeutic applications, like priming MSCs with stress conditions (hypoxia, nutrient deprivation) to achieve a higher therapeutic potential. The goal of the present study is to review the main findings regarding the MSCs isolated from the human vascular wall. Further, the main priming strategies tested on MSCs from different sources are reported, together with the experience on vascular MSCs isolated from healthy cryopreserved and pathological arteries. Stress induction can be a priming approach able to improve MSC effectiveness through several mechanisms that are discussed in this review. Nevertheless, these issues have not been completely explored in vascular MSCs and potential side effects need to be investigated.

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Section: Ethical Considerationsmentioning

confidence: 84%

The Characteristics and Survival Potential Under Sub-lethal Stress of Mesenchymal Stromal/Stem Cells Isolated from the Human Vascular Wall

2022

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“…The high correlation coefficients between the blood and cartilage tissues for methanol suggest that methanol concentration in cartilage can be determined, and methanol behaves similarly to urine. Since the obtained results add another perspective to the distribution of xenobiotics in cartilage tissues, they may also be important in the context of using this type of tissue for regenerative medicine [ 34 ] or plastic surgery purposes [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

Epiglottis Cartilage, Costal Cartilage, and Intervertebral Disc Cartilage as Alternative Materials in the Postmortem Diagnosis of Methanol Poisoning

Tomsia

Chełmecka

Głaz

et al. 2023

Toxics

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Alternative materials for postmortem diagnosis in the case of fatal poisonings are much needed when standard materials, such as blood and urine, are unavailable. The study presents a case of fatal mass methanol intoxication resulting from industrial alcohol consumption. The study aimed to determine methanol and formic acid concentrations in epiglottis cartilage, costal cartilage, and intervertebral disc cartilage and to analyze the correlation between their concentrations in cartilage tissues and the femoral blood. Methanol and formic acid concentrations in samples collected from 17 individuals (n = 17) were estimated using gas chromatography with flame ionization detection (GC-FID). Methanol concentration in the costal cartilage correlated with its concentration in the femoral blood (r = 0.871). Similar correlations were found for epiglottis cartilage (r = 0.822) and intervertebral disc cartilage (r = 0.892). Formic acid concentration in the blood correlated only with its concentration in urine (r = 0.784) and the epiglottis (r = 0.538). Cartilage tissue could serve as an alternative material for methanol analyses in postmortem studies. Formic acid, a methanol metabolite, does not meet the requirements for its presence determination in cartilage tissues.

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“…Post mortem tissues represent plentiful alternative sources for such cellular therapies, in particular from young donors without signs of age-associated joint degeneration. Previous studies have shown that primary cells with MSC-like properties can be obtained from various tissues, even several days post mortem [ 15 , 16 , 18 , 22 , 23 , 24 ], and from tissues stored under cryoprotection for years [ 25 ]. However, comprehensive studies that include several tissues from human post mortem donors along with specific times post mortem are scarce.…”

Section: Discussionmentioning

confidence: 99%

“…The tissues harvested from post mortem donors represent an alternative tissue source for isolating MSCs, particularly with the availability and ease of access to large numbers of biopsies [ 15 , 16 ]. Studies have shown that primary cells from various tissues of cadaveric donors can be isolated several hours, or even several days, post mortem [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Knee and Peri-Knee Tissues of Post Mortem Donors Are Strategic Sources of Mesenchymal Stem/Stromal Cells for Regenerative Procedures

Haring

Zupan

2022

IJMS

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Tissues of post mortem donors represent valuable alternative sources for the isolation of primary cells with mesenchymal stem/stromal cell (MSC)-like properties. However, the properties of primary cells derived from different tissues and at different post mortem times are poorly recognized. Here, we aim to identify the optimal tissue source between three knee and peri-knee tissues for the isolation of primary cells with MSC-like properties, and to define the influence of the time post mortem on the properties of these cells. We harvested tissues from subchondral bone marrow, synovium and periosteum from 32 donors at various post mortem times. Primary cells were evaluated using detailed in vitro analyses, including colony formation, trilineage differentiation, immunophenotyping and skeletal stem cell marker-gene expression profiling. These data show that the primary cells with MSC-like properties isolated from these three tissues show no differences in their properties, except for higher expression of CD146 in bone-marrow cells. The success rate of the primary cell isolation is dependent on the post mortem time. However, synovium and periosteum cells isolated more than 48 h post mortem show improved osteogenic and chondrogenic potential. This study suggests that knee and peri-knee tissues from donors even 3 days post mortem are strategic sources of MSCs for regenerative procedures.

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Cadaveric Stem Cells: Their Research Potential and Limitations

Cited by 10 publications

References 126 publications

The Characteristics and Survival Potential Under Sub-lethal Stress of Mesenchymal Stromal/Stem Cells Isolated from the Human Vascular Wall

The Characteristics and Survival Potential Under Sub-lethal Stress of Mesenchymal Stromal/Stem Cells Isolated from the Human Vascular Wall

Epiglottis Cartilage, Costal Cartilage, and Intervertebral Disc Cartilage as Alternative Materials in the Postmortem Diagnosis of Methanol Poisoning

Knee and Peri-Knee Tissues of Post Mortem Donors Are Strategic Sources of Mesenchymal Stem/Stromal Cells for Regenerative Procedures

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