Correlation between ELF–PEMF exposure and Human RPE Cell Proliferation, Apoptosis and Gene Expression

Abstract

Purpose: Emerging evidence implies that electromagnetic fields (EMFs) can negatively affect angiogenesis. In this regard, the effects of extremely low frequency pulsed electromagnetic field (ELF–PEMF) exposure on the relative expression level of angiogenic factors involved in the pathogenesis of ocular disorders were evaluated in human retinal pigment epithelial (hRPE) cells in order to investigate a noninvasive therapeutic method for patients with several ocular diseases associated with neovascularization.
 Methods: After separating hRPE cells from globes, hRPE cells were exposed to 15 mT of ELF–PEMF (120 Hz) at 5, 10, and 15 min for seven days. Cell proliferation and apoptosis of treated cells were evaluated via ELISA assay. Moreover, relative expression changes of HIF-1α, CTGF, VEGFA, MMP-2, cathepsin D, and E2F3 were performed using real-time RT-PCR.
 Results: ELF–PEMF exposure had no significant effects on the apoptosis and proliferation rate of hRPE cells. Expression level of HIF-1α, CTGF, VEGFA, MMP- 2, cathepsin D, and E2F3 was downregulated following 5 min of ELF–PEMF exposure.
 Conclusion: As ELF–PEMF showed inhibitory effects on the expression of angiogenic genes in hRPE cells with no cytotoxic or proliferative side effects, it can be introduced as a useful procedure for managing angiogenesis induced by retinal pathogenesis, although more studies with adequate follow-up in animal models are needed.

Niosome-encapsulated auraptene reduced the mRNA expression of VEGF-A and PDGFs genes in human retina-derived RPE cell line

“…The study was approved by the Ethics Committee of Golestan University of Medical Sciences (ethics code: IR.GOUMS.REC.1399.065). Cell Culture The present study utilized an RPE cell line that had undergone prior isolation and culturing procedures in our previous investigation [22] . The cells were cultured in T-25 flasks containing a complete culture medium containing 10% fetal bovine serum (FBS) and incubated at 37°C and 5% CO 2 after thawing.…”

“…The expression of VEGF-A, PDGF-A, PDGF-B, PDGF-C, PDGF-D, and GAPDH genes in cells treated with free niosome, free AUR, and niosomal AUR were measured using SYBR Green real-time polymerase chain reaction (RT-PCR, Yekta Tajhiz Azma Inc., Tehran, Iran). Table 1 contains the list of PCR primers [22,[24][25][26][27] . Statistical Analysis The 2 -∆∆Ct method was used to calculate the relative fold changes in gene expressions.…”

Cell Biological Effects of Long-term Exposure to Electromagnetic Field of Simulated Mobile Phones

“…Researches show a noteworthy elevation in the percentage of tail DNA in the testes under prolonged irradiation in comparison with other organs of rats [43]. ELF -PEMFs have a notable prohibitory function in the expression of angiogenic factors in hRPE cells as pivotal cells in eye health without cytotoxic or proliferative side effects and ELF -PEMF can be employed for therapeutic uses in ocular diseases [44].…”

Impact of umbelliprenin-containing niosome nanoparticles on VEGF-A and CTGF genes expression in retinal pigment epithelium cells

“…VEGF-A (located on 6p21.1 with 26 identified transcript) is the most critical factor in the angiogenesis process, which leads to an increase in the number of capillaries. As a signal protein, VEGF-A is produced by many tissues with insufficient blood circulation and functions as a part of the oxygen and nutrition delivery system [10] . Like other family members of VEGFs, it is highly conserved and considered as part of evolutionary distance between fish and mammals [11] .…”