Living in today’s world requires extensive communication with the world around us, which the mobile phone is one of this equipment along with other devices such as computers, radios, telephone towers, and power lines. It is supposed that prolonged exposure to radiation may cause biological damage to living organisms. However, the results from different laboratory studies are contradictory and there is still insufficient information about the biological risks to human health. In this article, we reviewed some of the potential biological effects of Radiofrequency Electromagnetic Field (RF-EMF) exposure.
Immunomagnetic beads (IMBs) for cell sorting are universally used in medical and biological fields. At present, the IMBs on the market are ferrite coated with a silicon shell. Based on a new type of magnetic material, the graphene coated iron nitride magnetic particle (G@FeN-MP), which we previously reported, we prepared a novel IMB, a graphene oxide coated iron nitride immune magnetic bead (GO@FeN-IMBs), and explored its feasibility for cell sorting. First, the surface of the G@FeN-MP was oxidized to produce oxygen-containing groups as carboxyl, etc. by the optimized Hummers’ method, followed by a homogenization procedure to make the particles uniform in size and dispersive. The carboxy groups generated were then condensed and coupled with anti-CD3 antibodies by the carbodiimide method to produce an anti-CD3-GO@FeN-IMB after the coupling efficacy was proved by bovine serum albumin (BSA) and labeled antibodies. Finally, the anti-CD3-GO@FeN-IMBs were incubated with a cell mixture containing human T cells. With the aid of a magnetic stand, the T cells were successfully isolated from the cell mixture. The isolated T cells turned out to be intact and could proliferate with the activation of the IMBs. The results show that the G@FeN-MP can be modified for IMB preparation, and the anti-CD3-GO@FeN-IMBs we prepared can potentially separate T cells.
This study uses the methods of expert interview, questionnaire survey, literature and other research methods to study the origin, the structure and the value of Bianlong. The research shows that the "back 8" movement of Bianlong has a special rehabilitation effect on shoulder periarthritis, cervical spondylosis and other joint diseases, and Bianlong has extremely high ornamental and commercial value. Some suggestions are put forward for the future development of Bianlong: formulating Bianlong courses and competition regulations, increasing the construction of Bianlong organization, and bringing Bianlong into the campus. It aims to lay the foundation for the development of Bianlong and academic research of Bianlong.
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