Enhanced cytotoxicity caused by AC magnetic field for polymer microcapsules containing packed magnetic nanoparticles

Zharkov, Mikhail N.; Brodovskaya, Ekaterina P.; Kulikov, Oleg A.; Gromova, Elena V.; Ageev, Valentin P.; Атанова, А. В.; Kozyreva, Zhanna V.; Tishin, A.M.; Pyatakov, A. P.; Pyataev, N. A.; Sukhorukov, Gleb B.

doi:10.1016/j.colsurfb.2020.111548

Cited by 19 publications

(18 citation statements)

References 65 publications

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“…The method of layer-by-layer adsorption of polyelectrolytes used for the formation of the polymer shell allows magnetic [21,22] and fluorescent semiconductor [10,13,23] nanoparticles and other fluorescent labels [24] to be incorporated into the shell, thus ensuring magnetic resonance or fluorescence imaging of the MCs. These polyelectrolyte structures can be efficiently optically encoded with fluorescent semiconductor nanocrystals (quantum dots (QDs)) [23,25].…”

Section: Introductionmentioning

confidence: 99%

Designing Functionalized Polyelectrolyte Microcapsules for Cancer Treatment

et al. 2021

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The engineering of delivery systems for drugs and contrasting labels ensuring the simultaneous imaging and treatment of malignant tumors is an important hurdle in developing new tools for cancer therapy and diagnosis. Polyelectrolyte microcapsules (MCs), formed by nanosized interpolymer complexes, represent a promising platform for the designing of multipurpose agents, functionalized with various components, including high- and low-molecular-weight substances, metal nanoparticles, and organic fluorescent dyes. Here, we have developed size-homogenous MCs with different structures (core/shell and shell types) and microbeads containing doxorubicin (DOX) as a model anticancer drug, and fluorescent semiconductor nanocrystals (quantum dots, QDs) as fluorescent nanolabels. In this study, we suggest approaches to the encapsulation of DOX at different stages of the MC synthesis and describe the optimal conditions for the optical encoding of MCs with water-soluble QDs. The results of primary characterization of the designed microcarriers, including particle analysis, the efficacy of DOX and QDs encapsulation, and the drug release kinetics are reported. The polyelectrolyte MCs developed here ensure a modified (prolonged) release of DOX, under conditions close to normal and tumor tissues; they possess a bright fluorescence that paves the way to their exploitation for the delivery of antitumor drugs and fluorescence imaging.

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Section: Introductionmentioning

confidence: 99%

Designing Functionalized Polyelectrolyte Microcapsules for Cancer Treatment

et al. 2021

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“…After being internalized by muscle cells, the capsules were found to eventually accumulate around the cell nuclei [ 43 ]. Upon interaction with live cells, QD-doped and MNP-doped polyelectrolyte microcapsules did not exhibit significant cytotoxicity, which resulted in a total cell viability of 80–90% in the cases of both normal and cancer cells [ 27 , 31 , 43 , 44 ]. These data confirm the efficacy of the encapsulation approach for enhancing the nanoparticle biocompatibility and demonstrate that nanoparticle-doped polyelectrolyte microcapsules can be used as agents to follow particle–cell interaction.…”

Section: Resultsmentioning

confidence: 99%

Nanoparticle-Doped Hybrid Polyelectrolyte Microcapsules with Controlled Photoluminescence for Potential Bioimaging Applications

et al. 2021

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Fluorescent imaging is widely used in the diagnosis and tracking of the distribution, interaction, and transformation processes at molecular, cellular, and tissue levels. To be detectable, delivery systems should exhibit a strong and bright fluorescence. Quantum dots (QDs) are highly photostable fluorescent semiconductor nanocrystals with wide absorption spectra and narrow, size-tunable emission spectra, which make them suitable fluorescent nanolabels to be embedded into microparticles used as bioimaging and theranostic agents. The layer-by-layer deposition approach allows the entrapping of QDs, resulting in bright fluorescent microcapsules with tunable surface charge, size, rigidity, and functional properties. Here, we report on the engineering and validation of the structural and photoluminescent characteristics of nanoparticle-doped hybrid microcapsules assembled by the deposition of alternating oppositely charged polyelectrolytes, water-soluble PEGylated core/shell QDs with a cadmium selenide core and a zinc sulfide shell (CdSe/ZnS), and carboxylated magnetic nanoparticles (MNPs) onto calcium carbonate microtemplates. The results demonstrate the efficiency of the layer-by-layer approach to designing QD-, MNP-doped microcapsules with controlled photoluminescence properties, and pave the way for the further development of next-generation bioimaging agents based on hybrid materials for continuous fluorescence imaging.

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“…Zharkov et al [297] proposed the design of hyperthermia platforms based on LbL capsules formed with dextran sulphate and poly-(L-arginine), and embedded iron oxide particles within the LbL shell. The obtained capsules were taken up by human fibroblasts upon the application of an alternating magnetic field, inducing a stronger cytotoxic effect than that of free magnetic nanoparticles.…”

Section: Layer-by-layer Capsules For Hyperthermia Treatmentsmentioning

confidence: 99%

Polyelectrolyte Multilayered Capsules as Biomedical Tools

et al. 2022

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Polyelectrolyte multilayered capsules (PEMUCs) obtained using the Layer-by-Layer (LbL) method have become powerful tools for different biomedical applications, which include drug delivery, theranosis or biosensing. However, the exploitation of PEMUCs in the biomedical field requires a deep understanding of the most fundamental bases underlying their assembly processes, and the control of their properties to fabricate novel materials with optimized ability for specific targeting and therapeutic capacity. This review presents an updated perspective on the multiple avenues opened for the application of PEMUCs to the biomedical field, aiming to highlight some of the most important advantages offered by the LbL method for the fabrication of platforms for their use in the detection and treatment of different diseases.

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Enhanced cytotoxicity caused by AC magnetic field for polymer microcapsules containing packed magnetic nanoparticles

Cited by 19 publications

References 65 publications

Designing Functionalized Polyelectrolyte Microcapsules for Cancer Treatment

Designing Functionalized Polyelectrolyte Microcapsules for Cancer Treatment

Nanoparticle-Doped Hybrid Polyelectrolyte Microcapsules with Controlled Photoluminescence for Potential Bioimaging Applications

Polyelectrolyte Multilayered Capsules as Biomedical Tools

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