The Layer-by-Layer (LbL) method is a well-established method for the assembly of nanomaterials with controlled structure and functionality through the alternate deposition onto a template of two mutual interacting molecules, e.g., polyelectrolytes bearing opposite charge. The current development of this methodology has allowed the fabrication of a broad range of systems by assembling different types of molecules onto substrates with different chemical nature, size, or shape, resulting in numerous applications for LbL systems. In particular, the use of soft colloidal nanosurfaces, including nanogels, vesicles, liposomes, micelles, and emulsion droplets as a template for the assembly of LbL materials has undergone a significant growth in recent years due to their potential impact on the design of platforms for the encapsulation and controlled release of active molecules. This review proposes an analysis of some of the current trends on the fabrication of LbL materials using soft colloidal nanosurfaces, including liposomes, emulsion droplets, or even cells, as templates. Furthermore, some fundamental aspects related to deposition methodologies commonly used for fabricating LbL materials on colloidal templates together with the most fundamental physicochemical aspects involved in the assembly of LbL materials will also be discussed.
This work presents a simple methodology
for coating small unilamellar
liposomes bearing different degrees of positive charge with polyelectrolyte
multilayers using the sequential layer-by-layer deposition method.
The liposomes were made of mixtures of 1,2-dioleyl-sn-glycero-3-phosphocoline and dimethyl dioctadecyl ammonium bromide
(DODAB) and coated by alternated layers of the sodium salt of poly(4-styrenesulfonate)
(PSS) and poly(allylamine) (PAH) as polyanions and polycations, respectively.
The results show that the zeta potential of the liposomes was not
very sensitive to the mole fraction of DODAB in the membrane, X
D, in the range 0.3 ≤ X
D ≤ 0.8. We were able to coat the liposomes with
up to four polymer bilayers. The growth of the capsule size was followed
by dynamic light scattering, and in some cases, by cryo-transmission
electron microscopy, with good agreement between both techniques.
The thickness of the layers, measured from the hydrodynamic radius
of the coated liposome, depends on the polyelectrolyte used, so that
the PSS layers adopt a much more packaged conformation than the PAH
layers. An interesting finding is that the PSS amount needed to reach
the isoelectric point of the capsules increases linearly with the
charge density of the bare liposomes, whereas the amount of PAH does
not depend on it. As expected, the preparation of the multilayers
has to be done in such a way that when the system is close to the
isoelectric point, the capsules do not aggregate. For this, we dropped
the polyelectrolyte solution quickly, stirred it fast, and used dilute
liposome suspensions. The method is very flexible and not limited
to liposomes or polyelectrolyte multilayers; also, coatings containing
charged nanoparticles can be easily made. Once the liposomes have
been coated, lipids can be easily eliminated, giving rise to polyelectrolyte
nanocapsules (polyelectrosomes) with potential applications as drug
delivery platforms.
Some biological microorganisms can crawl or swim due to coordinated motions of their cytoskeleton or the flagella located inside their bodies, which push the cells forward through intracellular forces. To date, there is no demonstration of synthetic systems propelling at low Reynolds number via the precise actuation of the material confined within an enclosing lipid membrane. Here, we report lipid vesicles and other more complex self-assembled biohybrid structures able to propel due to the advection flows generated by the actuated rotation of the superparamagnetic particles they contain. The proposed swimming and release strategies, based on cooperative hydrodynamic mechanisms and near-infrared laser pulse-triggered destabilization of the phospholipid membranes, open new possibilities for the on-command transport of minute quantities of drugs, fluid or nano-objects. The lipid membranes protect the confined substances from the outside environment during transportation, thus enabling them to work in physiological conditions.
Understanding the aggregation of magnetic particles is also essential for their use in the fabrication of metamaterials, [8] as magnetic separation agents in, e.g., protein purification protocols, [9] as contrast agents in magnetic resonance imaging, [10] or as cell manipulation operators [11] among others.Floating magnetic particles have also been extensively used in the bottom-up fabrication of different dynamic selfassemblies, which develop order at the same time as dissipate energy. The strong confinement of a system of particles can dramatically influence both the nature of their interactions and the long-range order possible, permitting the existence of new structures and phases both in equilibrium [12,13] and out of equilibrium. [12,14] Besides, the quasi 2D nature of these systems facilitates the experimental analysis of the structure and dynamics. In this regard, magnetic colloids adsorbed at liquid-liquid interphases have been proven to be suitable model systems to investigate the formation of static and dynamic arrangements of particles, and promising candidates to engineer novel functional planar structures unfeasible in bulk dipolar systems. [15,16,17] Grzybowski et al. studied disparate dynamic structures formed by rotating millimeter-sized magnetic disks, in processes ruled by the equilibrium between
Polyelectrolyte multilayered capsules (PEMUCs) obtained using the Layer-by-Layer (LbL) method have become powerful tools for different biomedical applications, which include drug delivery, theranosis or biosensing. However, the exploitation of PEMUCs in the biomedical field requires a deep understanding of the most fundamental bases underlying their assembly processes, and the control of their properties to fabricate novel materials with optimized ability for specific targeting and therapeutic capacity. This review presents an updated perspective on the multiple avenues opened for the application of PEMUCs to the biomedical field, aiming to highlight some of the most important advantages offered by the LbL method for the fabrication of platforms for their use in the detection and treatment of different diseases.
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