Enhanced cytotoxic, antioxidant and anti-inflammatory activities of curcumin diethyl disuccinate using chitosan-tripolyphosphate nanoparticles

Sorasitthiyanukarn, Feuangthit Niyamissara; Muangnoi, Chawanphat; Thaweesest, Wuttinont; Rojsitthisak, Pornchai

doi:10.1016/j.jddst.2019.06.015

Cited by 20 publications

(12 citation statements)

References 42 publications

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“…RAW 264.7 cells were seeded in 6-well plates at a density of 1.5 × 10 6 cells/well for 24 h. Then, cells were washed with phenol red-free medium and the medium was replaced with 2 mL of phenol red-free medium per well. The cells were then treated with 0.5% DMSO, curcumin, or CurDG at 5 µM (without toxicity) for 1 h, followed by stimulation with 1 µg/mL of LPS [ 31 ]. After incubation for 24 h, the culture medium was collected for determination of nitrite, TNF-α, and IL-6 levels.…”

Section: Methodsmentioning

confidence: 99%

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Molecular Insight into the Anti-Inflammatory Effects of the Curcumin Ester Prodrug Curcumin Diglutaric Acid In Vitro and In Vivo

Phumsuay

Muangnoi

Wasana

et al. 2020

IJMS

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Curcumin diglutaric acid (CurDG), an ester prodrug of curcumin, has the potential to be developed as an anti-inflammatory agent due to its improved solubility and stability. In this study, the anti-inflammatory effects of CurDG were evaluated. The effects of CurDG on inflammatory mediators were evaluated in LPS-stimulated RAW 264.7 macrophage cells. CurDG reduced the increased levels of NO, IL-6, and TNF- α, as well as iNOS and COX-2 expression in cells to a greater extent than those of curcumin, along with the potent inhibition of MAPK (ERK1/2, JNK, and p38) activity. The anti-inflammatory effects were assessed in vivo by employing a carrageenan-induced mouse paw edema model. Oral administration of CurDG demonstrated significant anti-inflammatory effects in a dose-dependent manner in mice. The effects were significantly higher compared to those of curcumin at the corresponding doses (p < 0.05). Moreover, 25 mg/kg curcumin did not exert a significant anti-inflammatory effect for the overall time course as indicated by the area under the curve data, while the equimolar dose of CurDG produced significant anti-inflammatory effects comparable with 50, 100, and 200 mg/kg curcumin (p < 0.05). Similarly, CurDG significantly reduced the proinflammatory cytokine expression in paw edema tissues compared to curcumin (p < 0.05). These results provide the first experimental evidence for CurDG as a promising anti-inflammatory agent.

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Section: Methodsmentioning

confidence: 99%

“…Then, 50 μL of N -1-Napthylenediamine dihydrochloride (NED) solution was added, incubated for 5 min, then the absorbance was measured at 520 nm. The concentration of NO was determined by comparison to the standard curve of NaNO 2 [ 31 ].…”

Section: Methodsmentioning

confidence: 99%

Molecular Insight into the Anti-Inflammatory Effects of the Curcumin Ester Prodrug Curcumin Diglutaric Acid In Vitro and In Vivo

Phumsuay

Muangnoi

Wasana

et al. 2020

IJMS

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“…For instance, ionotropic gelation is one of the major techniques assumed for the synthesis of polymers nanoparticles. [112] Others techniques based on solvent evaporation were also developed. [113] Materials based on polyelectrolytes are nowadays highly studied.…”

Section: Nanoparticlesmentioning

confidence: 99%

Recent Advances in Antiinflammatory Material Design

Lebaudy

Fournel

Lavalle

et al. 2020

Adv Healthcare Materials

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Implants and prostheses are widely used to replace damaged tissues or to treat various diseases. However, besides the risk of bacterial or fungal infection, an inflammatory response usually occurs. Here, recent progress in the field of anti‐inflammatory biomaterials is described. Different materials and approaches are used to decrease the inflammatory response, including hydrogels, nanoparticles, implant surface coating by polymers, and a variety of systems for anti‐inflammatory drug delivery. Complex multifunctional systems dealing with inflammation, microbial infection, bone regeneration, or angiogenesis are also described. New promising stimuli‐responsive systems, such as pH‐ and temperature‐responsive materials, are also being developed that would enable an “intelligent” antiinflammatory response when the inflammation occurs. Together, different approaches hold promise for creation of novel multifunctional smart materials allowing better implant integration and tissue regeneration.

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“…The technique that can be used to increase the biological activity of compounds is by encapsulating in nanometric sizes [7]. Previous studies have shown that curcumin diethyl disuccinate encapsulated by chitosan-tripolyphosphate nanoparticles has better antioxidant activity when compared to without encapsulation [9]. As well as clove essential oil [CEO] encapsulated in chitosan nanoparticles by using the emulsion-ionic gelation method has higher antioxidant activity than free CEO [10].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Encapsulated Chromolaena Odorata Leaf Extract in Chitosan Nanoparticle by Using Ionic Gelation Method and Its Antioxidant Activity

2021

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Objective: The aim of this study was to determine the antioxidant activity of Chromolaena odorata. Methods: Encapsulation of Chromolaena odorata leaf extract by nano chitosan was synthesized by using chitosan and NaTPP as the crosslinking agent. The antioxidant activity was conducted by using the DPPH method. Results: Nanoparticles of Chromolaena odorata leaf extract has an average diameter of 675±218 nm and+23.4±7.14 mV of zeta potential. The antioxidant activity of its extract was 0.86 ppm, while its nanoparticle has the better antioxidant activity of 0.21 ppm. Conclusion: Nanoparticles of Chromolaena odorata have very strong antioxidant activity and the potential to be external antioxidants.

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Enhanced cytotoxic, antioxidant and anti-inflammatory activities of curcumin diethyl disuccinate using chitosan-tripolyphosphate nanoparticles

Cited by 20 publications

References 42 publications

Molecular Insight into the Anti-Inflammatory Effects of the Curcumin Ester Prodrug Curcumin Diglutaric Acid In Vitro and In Vivo

Molecular Insight into the Anti-Inflammatory Effects of the Curcumin Ester Prodrug Curcumin Diglutaric Acid In Vitro and In Vivo

Recent Advances in Antiinflammatory Material Design

Synthesis of Encapsulated Chromolaena Odorata Leaf Extract in Chitosan Nanoparticle by Using Ionic Gelation Method and Its Antioxidant Activity

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