Enhanced cephalosporin C production with a combinational ammonium sulfate and DO-Stat based soybean oil feeding strategy

Duan, Shengbing; Gao, Yuan; Zhao, Yanli; Li, Hongfei; Ni, Weijia; Sang, Meina; Liu, Li; Shi, Zhongping

doi:10.1016/j.bej.2011.11.011

Cited by 3 publications

(1 citation statement)

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“…Oil is rich in fatty acids, which can be degraded into acyl-coenzymes A by microbes. Acyl-coenzymes A are recognized as important precursors for the biosynthesis of secondary metabolites in Streptomyces (Chen et al 2012; Duan et al 2012; Lu et al 2016). As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Enhancement of ascomycin production via a combination of atmospheric and room temperature plasma mutagenesis in Streptomyces hygroscopicus and medium optimization

Shen

et al. 2019

AMB Expr

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Ascomycin, a key intermediate for chemical synthesis of immunosuppressive drug pimecrolimus, is produced by Streptomyces hygroscopicus var. ascomyceticus . In order to improve the strain production, the original S. hygroscopicus ATCC 14891 strain was treated here with atmospheric and room temperature plasma to obtain a stable high-producing S. hygroscopicus SFK-36 strain which produced 495.3 mg/L ascomycin, a 32.5% increase in ascomycin compared to the ATCC 14891. Then, fermentation medium was optimized using response surface methodology to further enhance ascomycin production. In the optimized medium containing 81.0 g/L soluble starch, 57.4 g/L peanut meal, and 15.8 g/L soybean oil, the ascomycin yield reached 1466.3 mg/L in flask culture. Furthermore, the fermentation process was carried out in a 5 L fermenter, and the ascomycin yield reached 1476.9 mg/L, which is the highest ascomycin yield reported so far. Therefore, traditional mutagenesis breeding combined with medium optimization is an effective approach for the enhancement of ascomycin production. Electronic supplementary material The online version of this article (10.1186/s13568-019-0749-x) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%