Enhanced Cells Anchoring to Electrospun Hybrid Scaffolds With PHBV and HA Particles for Bone Tissue Regeneration

Abstract

Hybrid materials combining organic and inorganic compounds used as scaffolds are highly beneficial in bone regeneration. In this study, we successfully produced by blend electrospinning poly(3-hydroxybutyric acid-co-3-hydrovaleric acid) (PHBV) scaffolds enriched with hydroxyapatite (HA) particles to biomimic bone tissue for improved and faster regeneration processes. The morphology, fiber diameters, and composition of the scaffolds were investigated by scanning electron microscopy (SEM) techniques followed by focused ion beam (FIB) sectioning to verify HA particles integration with PHBV fibers. In vitro cell culture was performed for 7 days and followed with the cell proliferation test (CellTiter-Blue® Assay). Additionally, cell integration with the scaffold was visualized by confocal and SEM imaging. We developed a simple way of obtaining hybrid scaffolds by electrospinning PHBV solution with HA particles without any post-processing. The PHBV + HA scaffold enhanced cell proliferation and filopodia formation responsible for cell anchoring within the created 3D environment. The obtained results show the great potential in the development of hybrid scaffolds stimulating bone tissue regeneration.

Fabrication and Characterization of Cinnamaldehyde-Loaded Mesoporous Bioactive Glass Nanoparticles/PHBV-Based Microspheres for Preventing Bacterial Infection and Promoting Bone Tissue Regeneration

“…The EDS spectrum of SBF immersed PHBV/MBGN/CIN20 microspheres confirmed the presence of Ca and P [ 82 ]. The peak of C corresponds to the polymeric matrix (PHBV) [ 69 , 83 ]. These results confirm the bioactivity of MBGN incorporated in PHBV/MBGN/CIN20 microspheres.…”

Development and Advantages of Biodegradable PHA Polymers Based on Electrospun PHBV Fibers for Tissue Engineering and Other Biomedical Applications

“…Copyright 2020 Elsevier. Panels E and F reproduced with permission from ref ( 166 ). Copyright 2021 Karbowniczek, Kaniuk, Berniak, Gruszczyński, and Stachewicz.…”

“…The fibrous PHBV scaffolds enriched with inorganic HA particles were seen to support the anchoring and attachment of osteoblasts (see Figure 7 E,F). 166 It has been shown that the inclusion of HA particles in the fiber structure increases cell proliferation. These studies confirmed that the combination of organic and inorganic materials produces a hybrid material, the properties of which can support bone regeneration.…”

“…These studies confirmed that the combination of organic and inorganic materials produces a hybrid material, the properties of which can support bone regeneration. 166 Zhang et al 173 used another approach to support the regeneration of bone tissue: a scaffold based on PHBV, chitosan, and HA particles. Calcium deposition in HA-enriched scaffolds, as confirmed by EDX analysis, may reflect the bone mineralization process.…”

Fibrous Polymer-Based Composites Obtained by Electrospinning for Bone Tissue Engineering

“…The results of transmission electron microscopy have demonstrated that the authors managed to obtain ultrafine fibers with average diameter of approximately 200 nm and uniform distribution of HAP-based clusters ( Figure 2 ). In [ 78 ], the authors used blend electrospinning to produce poly(3-hydroxybutyric acid-co-3-hydrovaleric acid) (PHBV) filled with HAP particles. The composition and structural characteristics, including fiber diameter and surface morphology, were observed by means of scanning electron microscopy (SEM) supported by focused ion beam (FIB) application to confirm the presence of HAP inclusions and its integration with polymer matrix.…”