Enhanced cell survival of melanocyte spheroids in serum starvation condition

Lin, Sung‐Jan; Jee, Shiou‐Hwa; Hsiao, Wen-Chu; Yu, Hsin‐Su; Tsai, Tsen‐Fang; Chen, Jau-Shiuh; Hsu, Chih-Chieh; Young, Tai‐Horng

doi:10.1016/j.biomaterials.2005.08.031

Cited by 37 publications

(38 citation statements)

References 42 publications

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“…It has been shown that the function, proliferation and viability of cells are related to the morphology and intercellular organization of cells [17][18][19]. We have shown that, in comparison with monolayered melanocytes, melanocyte spheroids have a better survival in stringent conditions [12].…”

Section: Biomedical Engineering-applications Basis and Communicationsmentioning

confidence: 76%

“…To overcome drawbacks using melanocyte suspension, we have proposed the concept of using chitosan-based melanocyte patch for melanocyte transplantation [11][12]. The melanocyte patch is made by preculturing melanocytes on a chitosan membrane and the chitosan-based melanocyte patch can be used to cover the lesional sites with an upside-down orientation during transplantation.…”

Section: Biomedical Engineering-applications Basis and Communicationsmentioning

confidence: 99%

“…Further, in the presence fetal calf serum, human melanocytes form three dimensional multicellular spheroids on chitosan-coated surface when an appropriate seeding density is used [11]. Functionally, human melanocytes have a better survival in growth factor and serum deprived condition when they are precultured into multicellular spheroids on chitosan-coated surface [12]. Hence, chitosan-based spheroidal melanocyte patch may provide melanocytes a survival advantage in stringent conditions and it can theoretically improve the engraftment rates of melanocyte transplantation.…”

Section: Biomedical Engineering-applications Basis and Communicationsmentioning

confidence: 99%

“…It has been shown that certain serum signaling factors are crucial to multicellular spheroid formation in certain cancer cell lines [13][14]. It is notable that animal-derived serum in used in our culture system [11][12]. In terms of clinical application, the avoidance of animal serum in culture condition can prevent possible transfection of infectious disease from animal to human.…”

Section: Biomedical Engineering-applications Basis and Communicationsmentioning

confidence: 99%

“…When initial seeding density exceeds a critical level, melanocytes spontaneously grow into spheroids within 3 days on chitosan surface [11]. In addition, in collagen-coated wells or tissue culture polystyrene wells, melanocytes can not maintain a spheroidal morphology [11][12].…”

Section: Biomedical Engineering-applications Basis and Communicationsmentioning

confidence: 99%

See 4 more Smart Citations

The Effect of Serum Concentration on the Spheroid Forming Activity and Cell Growth of Human Melanocytes on Chitosan Surface

Lin

Hsiao

Hsu

et al. 2006

Biomed. Eng. Appl. Basis Commun.

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Section: Biomedical Engineering-applications Basis and Communicationsmentioning

confidence: 76%

Section: Biomedical Engineering-applications Basis and Communicationsmentioning

confidence: 99%

Section: Biomedical Engineering-applications Basis and Communicationsmentioning

confidence: 99%

Section: Biomedical Engineering-applications Basis and Communicationsmentioning

confidence: 99%

Section: Biomedical Engineering-applications Basis and Communicationsmentioning

confidence: 99%

See 3 more Smart Citations

The Effect of Serum Concentration on the Spheroid Forming Activity and Cell Growth of Human Melanocytes on Chitosan Surface

Lin

Hsiao

Hsu

et al. 2006

Biomed. Eng. Appl. Basis Commun.

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Bottom‐up Nanoencapsulation from Single Cells to Tunable and Scalable Cellular Spheroids for Hair Follicle Regeneration

Wang

Miao

Huang

et al. 2017

Adv Healthcare Materials

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Cell surface engineering technology advances cell therapeutics and tissue engineering by accurate micro/nanoscale control in cell-biomaterial ensembles and cell spheroids formation. By tailoring cell surface, microgels can encapsulate cells for versatile uses. However, microgels are coated in a thick layer to house multiple cells together but not a single cell based. Besides, excessive deposition on cell surface is detrimental to cellular functions. Herein, layer-by-layer (LbL) self-assembly to encapsulate single cell using nanogel is reported, owing to its security and tunable thickness at nanoscale, and further forms cell spheroids by physical cross-linking on nanogel-coated cells for delivery. A hair follicle (HF) regeneration model where the dermal papilla cells (DPCs) are given a 3D installation to maintain its ability of HF induction during in vitro culture is studied. Dermal papilla (DP) spheroids are optimized and that LbL-DPCs aggregation is akin to primary DP is demonstrated. The markers ALP, Versican, and NCAM are examined to investigate that high-passaged (P8) DP spheroids can restore the hair induction potential, which are lost in 2D culture. New HFs are regenerated successfully by implantation of DP spheroids in vivo.

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Transferable crosslinked chitosan membranes for human melanocyte culture

Dong

Wang

et al. 2011

J Biomedical Materials Res

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Development of transferable biocompatible membranes, which can be used for melanocyte culture and transplantation, is considered a feasible approach to increase the success rate for vitiliginous treatment. In this study, a crosslinked chitosan membrane (CCM) was produced via physical crosslinking of chitosan with sodium sulfate. The physical and mechanical properties as well as growth and phenotype expression of melanocytes on the CCM were investigated. The CCM supported growth and proliferation of melanocytes with the existence of melanin granules in the cytoplasm. The melanocytes remained active after transplantation. The CCM absorbed water approximately doubled from its original weight and permitted ∼2400 g/m² per day of water vapor transmission, suggesting that the CCM can function as an efficient wound dressing. Dynamic mechanical and tensile measurement results showed that the CCM possessed favorable wet strength for cell culture, separation, transfer, and transplantation application. This transferable CCM has the potential to be applied for vitiligo treatment.

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Enhanced cell survival of melanocyte spheroids in serum starvation condition

Cited by 37 publications

References 42 publications

The Effect of Serum Concentration on the Spheroid Forming Activity and Cell Growth of Human Melanocytes on Chitosan Surface

The Effect of Serum Concentration on the Spheroid Forming Activity and Cell Growth of Human Melanocytes on Chitosan Surface

Bottom‐up Nanoencapsulation from Single Cells to Tunable and Scalable Cellular Spheroids for Hair Follicle Regeneration

Transferable crosslinked chitosan membranes for human melanocyte culture

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