We concluded that only cell-cell interactions dominated in melanocytes seeded on the PVA membrane, whereas cell-cell and cell-substrate interactions occurred on the chitosan membranes and further enhanced cellular functions. The chitosan-induced spheroids could probably overcome the diffusion and assimilation of trophic factors.
Articles you may be interested inSurface modification of silicon-containing fluorocarbon films prepared by plasma-enhanced chemical vapor deposition J. Vac. Sci. Technol. A 23, 666 (2005); 10.1116/1.1943450 Effect of fluorine incorporation on silicon dioxide prepared by high density plasma chemical vapor deposition with SiH 4 ∕ O 2 ∕ NF 3 chemistry High-density plasma chemical-vapor deposition phosphosilicate glass ͑PSG͒ films were evaluated for the application of premetal dielectric materials. The PSG films were deposited using phosphorous-related precursors reacted with silane and oxygen at a temperature ഛ550°C. The as-deposited films were subsequently furnace annealed at 400°C for 10 to 30 min to simulate the effect of thermal budget on premetal dielectric layers in the current integrated circuit scheme. In addition, the PSG films were also annealed by rapid thermal processing at 925°C for 10 to 30 s, to examine film stability near the glass transformation temperature. Fourier transform infrared spectroscopy ͑FTIR͒, stress measurement, x-ray fluorescence analysis, and x-ray photoelectron spectroscopy ͑XPS͒ were used to characterize the PSG films. Film stress measurement was used to examine the stress hysteresis of the PSG films in the thermal-budget process. The results show that residual inactive phosphorous and compounds with P v O bonds are present in the as-deposited PSG films. Some residual phosphorous became active after the thermal annealing. The FTIR results that show an increase in the P v O group upon numerous annealing treatments is in agreement with the XPS analysis.
The purpose of this work is to investigate the microstructure of blended materials using non-invasive, optical imaging modality. Multiphoton autofluorescence and second harmonic generation signals will be used for characterizing and quantifying individual non-linear optical properties of each polymer in pure polymeric thin films. In addition, reflected confocal signals will used to outline the interface of refractive index mismatch. And the phase separation phenomenon of immiscible blended membranes composed of different ratio of nylon and chitosan are analyzed and differentiated using the non-invasive optical information including autofluerescence, second harmonic generation, and reflected signals. We therefore propose the potentiality of using multiphoton autofluorescence and second harmonic generation microscopy complemented with reflected confocal microscopy for studying the synthetic blended polymeric scaffolds and also, in the future, the dynamic, in vivo, cell-matrix interaction in the field of tissue engineering.
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