Enhanced bioactive compound production in broccoli cells due to coronatine and methyl jasmonate is linked to antioxidative metabolism

Sánchez-Pujante, Pedro Joaquín; Gionfriddo, Matteo; Sabater-Jara, Ana Belén; Almagro, Lorena; Pedreño, María A.; Díaz‐Vivancos, Pedro

doi:10.1016/j.jplph.2020.153136

Cited by 16 publications

(11 citation statements)

References 86 publications

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“…In that study, the highest amount of 6 had been obtained in the C (140.40 mg/100 g FW), while after treatment elicitors, the amount of 6 decreases with the maximum amount obtained for 0.5 g/L casein hydrolysate (68.00 mg/100 g FW). The influence of elicitors on the GSL content had also been the object of a study by Sánchez-Pujante et al (2020) on Brassica oleracea L. var. italica suspension cell cultures.…”

Section: Discussionmentioning

confidence: 99%

Impacts of elicitors on metabolite production and on antioxidant potential and tyrosinase inhibition in watercress microshoot cultures

Klimek-Szczykutowicz

Dziurka

Blažević

et al. 2022

Appl Microbiol Biotechnol

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The study has proved the stimulating effects of different strategies of treatments with elicitors on the production of glucosinolates (GSLs), flavonoids, polyphenols, saccharides, and photosynthetic pigments in watercress (Nasturtium officinale) microshoot cultures. The study also assessed antioxidant and anti-melanin activities. The following elicitors were tested: ethephon (ETH), methyl jasmonate (MeJA), sodium salicylate (NaSA), and yeast extract (YeE) and were added on day 10 of the growth period. Cultures not treated with the elicitor were used as control. The total GSL content estimations and UHPLC-DAD-MS/MS analyses showed that elicitation influenced the qualitative and quantitative profiles of GSLs. MeJA stimulated the production of gluconasturtiin (68.34 mg/100 g dried weight (DW)) and glucobrassicin (65.95 mg/100 g DW). The elicitation also increased flavonoid accumulation (max. 1131.33 mg/100 g DW, for 100 μM NaSA, collection after 24 h). The elicitors did not boost the total polyphenol content. NaSA at 100 μM increased the production of total chlorophyll a and b (5.7 times after 24 h of treatment), and 50 μM NaSA caused a 6.5 times higher production of carotenoids after 8 days of treatment. The antioxidant potential (assessed with the CUPRAC FRAP and DPPH assays) increased most after 24 h of treatment with 100 μM MeJA. The assessment of anti-melanin activities showed that the microshoot extracts were able to cause inhibition of tyrosinase (max. 27.84% for 1250 µg/mL). Key points • Elicitation stimulated of the metabolite production in N. officinale microshoots. • High production of pro-health glucosinolates and polyphenols was obtained. • N. officinale microshoots have got tyrosinase inhibition potential. • The antioxidant potential of N. officinale microshoots was evaluated. Graphical abstract

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Section: Discussionmentioning

confidence: 99%

Impacts of elicitors on metabolite production and on antioxidant potential and tyrosinase inhibition in watercress microshoot cultures

Klimek-Szczykutowicz

Dziurka

Blažević

et al. 2022

Appl Microbiol Biotechnol

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“…This gave Poland a place among the leading producers in Europe, with Spain leading the way. The consumption per person is growing steadily [1,2]. Broccoli owes its growing popularity to the fact that it is a rich source of health promoting compounds, showing high radical scavenging activity [3][4][5] and potential anti-cancer properties [6,7].…”

Section: Introductionmentioning

confidence: 99%

Response of Broccoli Transplants to LED Light during Short and Long-Term Storage

et al. 2020

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In broccoli production a big challenge is preparation of seedlings, which must be delivered during a strictly defined period to vegetable producers that are often in distant places. The goal of the present study was to investigate the response of 5-week old broccoli transplants cv. Parthenon F1 to 2 and 6 weeks of storage at 4 °C under two LED light spectra (L1: 29% red 660 nm + 14% red 630 nm + 42% blue 250 nm + 15% green 520 nm and L2: 38% red 660 nm + 18% red 630 nm +26% blue 450 nm + 15% green 520 nm + 3% UV-A 330 nm; photoperiod 16/8 h day/night and PPFD 30 ± 10 µmol m−2 s−1) and darkness. Control 5-week-old seedlings were not stored. Light treatment improved the morphology and physiological parameters of seedlings. The yield of broccoli from control seedlings was similar to that obtained after L2 treatment, regardless of seedling storage duration. The most promising effects were obtained by adding 3% UV-A radiation to the lighting spectrum. The results are applicable to the transport of broccoli transplants to remote locations or storage for several weeks, in cases where it is necessary to delay planting.

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“…In contrast, anthocyanin concentration was significantly higher in treated HRs at day 20-as was also shown in Figure 4-as well as at day 25, with increases of around 20% in both cases (Figure 5b). Taking into account that many elicitor treatments leading to enhanced secondary metabolites production have been linked to the establishment of a moderate oxidative stress [22,[26][27][28], the activity of the main antioxidant enzymes was determined in 43-R line for untreated HRs, and for the two treatments that provided the highest and the lowest anthocyanin concentrations (200 µM H 2 O 2 applied on day 12 and 200 µM H 2 O 2 applied on day 10, respectively). The elicitation process has been linked to controlled ROS overaccumulation and oxidative burst in in vitro systems, including HRs, in which antioxidant enzymes may play a key role [22,26,27,49,50].…”

Section: Resultsmentioning

confidence: 99%

“…For instance, in broccoli cells, enhanced bioactive compound production was achieved by coronatine-and methyl jasmonate-mediated mild oxidative stress. In these elicited broccoli suspension-cultured cells, the increase in glucosinolates and phenolic compounds was correlated with higher ascorbate peroxidase (APX) activity (a H 2 O 2 -scavenging enzyme), as well as with lower monodehydroascorbate reductase (MDHAR) and glutathione reductase (GR) activities, these enzymes being part of the AsA-GSH pathway [28].…”

Section: Resultsmentioning

confidence: 99%

“…Secondary metabolite accumulation in HR cultures is often triggered by elicitors [21][22][23][24][25], which may function as signaling molecules of plant stress responses. Many elicitor treatments have been linked with reactive oxygen species (ROS) over-accumulation, especially hydrogen peroxide (H 2 O 2 ), leading to an oxidative stress that directly activates secondary metabolite formation [22,[26][27][28]. Plant responses to H 2 O 2 are of great interest due to the role of H 2 O 2 as a signaling molecule, being perceived as an elicitor that, when added exogenously, may trigger an oxidative stress leading to secondary metabolites accumulation [29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

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H2O2-Elicitation of Black Carrot Hairy Roots Induces a Controlled Oxidative Burst Leading to Increased Anthocyanin Production

Barba‐Espín

Martínez-Jiménez

Izquierdo-Martínez

et al. 2021

Plants

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Hairy roots (HRs) grown in vitro are a powerful platform for plant biotechnological advances and for the bio-based production of metabolites of interest. In this work, black carrot HRs able to accumulate anthocyanin as major secondary metabolite were used. Biomass and anthocyanin accumulation were improved by modulating growth medium composition—different Murashige & Skoog (MS)-based media—and H2O2-elicitation, and the level of the main antioxidant enzymes on elicited HRs was measured. Higher growth was obtained on liquid 1/2 MS medium supplemented with 60 g/L sucrose for HRs grown over 20 days. In this medium, 200 µM H2O2 applied on day 12 induced anthocyanin accumulation by 20%. The activity of superoxide dismutase (SOD)—which generates H2O2 from O2•−—increased by over 50%, whereas the activity of H2O2-scavenging enzymes was not enhanced. Elicitation in the HRs can result in a controlled oxidative burst, in which SOD activity increased H2O2 levels, whereas anthocyanins, as effective reactive oxygen species scavengers, could be induced to modulate the oxidative burst generated. Moreover, given the proven stability of the HR lines used and their remarkable productivity, this system appears as suitable for elucidating the interplay between antioxidant and secondary metabolism.

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Enhanced bioactive compound production in broccoli cells due to coronatine and methyl jasmonate is linked to antioxidative metabolism

Cited by 16 publications

References 86 publications

Impacts of elicitors on metabolite production and on antioxidant potential and tyrosinase inhibition in watercress microshoot cultures

Impacts of elicitors on metabolite production and on antioxidant potential and tyrosinase inhibition in watercress microshoot cultures

Response of Broccoli Transplants to LED Light during Short and Long-Term Storage

H2O2-Elicitation of Black Carrot Hairy Roots Induces a Controlled Oxidative Burst Leading to Increased Anthocyanin Production

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