Enhanced anthocyanin production in grape cell cultures

Hirasuna, Thomas J.; Shuler, Michael L.; Lackney, Vincent K.; Spanswick, Roger M.

doi:10.1016/0168-9452(91)90167-7

Cited by 72 publications

(32 citation statements)

References 33 publications

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“…[6][7][8][9] Cell cultures were placed in an enclosed polyacrylic labeling chamber, constructed to provide a safe containment space as labeled polyphenolic compounds were biosynthesized by the cells. 8 Uniformly labeled […”

Section: Preparation Of 14 C-labeled Grape Polyphenol Fractionsmentioning

confidence: 99%

Pharmacokinetics and Tissue Distribution of¹⁴C-Labeled Grape Polyphenols in the Periphery and the Central Nervous System Following Oral Administration

Janle

Lila

Grannan

et al. 2010

Journal of Medicinal Food

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Grape polyphenols confer potential health benefits, including prevention of neurodegenerative diseases. To determine the absorption and tissue distribution of the complex grape polyphenol mixture, 14 C-labeled polyphenols were biosynthesized by grape cell suspension cultures, during co-incubation with radioisotopically labeled sucrose, and fractionated into polyphenolic subfractions. The pharmacokinetics and distribution of grape polyphenols into blood, brain, and peripheral interstitial fluid were determined by tracking the 14 C label. The blood peak 14 C concentration of the fractions ranged from 15 minutes to 4 hours. Absorption and tissue distribution varied greatly between fractions. Concentrations in interstitial fluid were lower than in blood. The amount of residual label in the brain at 24 hours ranged from 0.1% to 1.7% of the dose, depending on the fraction. 14 C label found in the brain tissue and brain microdialysate indicated that grape polyphenols or their metabolites are able to cross the blood-brain barrier. Using 14 C-labeled plant polyphenols it is possible to track the compounds or their metabolic products into any tissue and determine distribution patterns in spite of low concentrations. A central question regarding the potential role of dietary polyphenolics in neurodegenerative research is whether they are bioavailable in the brain. Our observations indicate that some grape-derived polyphenolics do reach the brain, which suggests their potential value for applications in neurodegenerative disorders.

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Section: Preparation Of 14 C-labeled Grape Polyphenol Fractionsmentioning

confidence: 99%

Pharmacokinetics and Tissue Distribution of¹⁴C-Labeled Grape Polyphenols in the Periphery and the Central Nervous System Following Oral Administration

Janle

Lila

Grannan

et al. 2010

Journal of Medicinal Food

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“…Anthocyanin level was lower as in other cell culture systems like rabbiteye blueberry (Nawa et al, 1993). Higher sucrose levels and lower nitrate levels enhanced anthocyanin accumulation in parallel with other cell culture systems like grapes (Do and Cormier, 1991;Hirasuna et al, 1991). Previous studies in our lab- oratory have indicated that higher sucrose levels were necessary to induce anthocyanin production in cranberry cultures (Madhavi et al, 1993).…”

Section: Other Flavonoidsmentioning

confidence: 72%

Expression of Anthocyanins in Callus Cultures of Cranberry (Vaccinium macrocarpon Ait)

Madhavi

Smith

Berber-Jimenez

1995

Journal of Food Science

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Expression of anthocyanins and other flavonoids in callus cultures established from different parts of cranberry plant was investigated and the effect of explant source on the in vitro product was determined. Callus cultures were initiated from different parts of the plant in a modified Gamborg's medium with 5.37 pM o-naphthaleneacetic acid, 0.45 pM 2,4-dichlorophenoxyacetic acid, and 2.32 pM kinetin in the dark at 25°C. Callus cultures accumulated anthocyanins only on exposure to light and maximum concentration was observed by day 12. The cultures had lower levels of anthocyanins and only cyanidin 3-galactoside, cyanidin 3-glucoside, and cyanidin 3-arabinoside were identified in all cultures regardless of source of explant. Proanthocyanidin accumulation in cultures was independent of light, and levels were higher than in mature fruit. Exposure to light induced accumulation of flavonols and enhanced activity of phenylalanine ammonia-lyase in the cultures.

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“…The effect of NH 4 ? :NO 3 -ratio on the growth kinetics of cells and their secondary metabolism with phenolic structure for some cell cultures has been reported (Do and Cormier 1991;Hirasuna et al 1991;Srinivasan and Ryn 1993) including the production of RA by Anchusa officinalis (De-Eknamkul and Ellis 1985) and Lavandula vera (Ilieva and Pavlov 1999). Usually the start of the biosynthesis of secondary metabolites is related to comparatively low levels of nitrogen in the medium.…”

Section: Discussionmentioning

confidence: 99%

Establishment and characterization of a Satureja khuzistanica Jamzad (Lamiaceae) cell suspension culture: a new in vitro source of rosmarinic acid

et al. 2015

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An in vitro approach to the production of rosmarinic acid (RA), a medicinally important caffeic acid ester, in a cell suspension culture (CSC) of Satureja khuzistanica Jamzad (Lamiaceae) has been investigated for the first time. The CSC was established from friable calli derived from shoot tip explants in Gamborg's B5 liquid medium supplemented with 30 g/L sucrose, 20 mg/L L-glutamine, 200 mg/L casein hydrolysate, 5 mg/L benzyladenine (BA) and 1 mg/L indole-3-butyric acid (IBA). The effect of nitrogen source (KNO 3 and (NH 4 ) 2 SO 4 ) and their different concentrations on the fresh and dry weight (g/L), as well as RA content (mg/g dry weight)were measured. CSC growth measurements indicated a maximum specific cell growth rate of 1.5/day, a doubling time of 7.6 days and a high percentage of cell viability (96.4 %) throughout the growth cycle. Maximum cell fresh weight (353.5 g/L), dry weight (19.7 g/L) and RA production (180.0 mg/g) were attained at day 21 of culture. Cell growth and RA content were affected by nitrogen deficiency. Media containing 8.3 mM of total nitrogen ( of B5 standard medium) led to a minimum cell fresh weight (243.0 g/ L), dry weight (17.4 g/L) and RA content (38.0 mg/g) after 21 days. The established CSC provided useful material for further optimization experiments aimed at a large-scale production of RA.

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Enhanced anthocyanin production in grape cell cultures

Cited by 72 publications

References 33 publications

Pharmacokinetics and Tissue Distribution of¹⁴C-Labeled Grape Polyphenols in the Periphery and the Central Nervous System Following Oral Administration

Pharmacokinetics and Tissue Distribution of¹⁴C-Labeled Grape Polyphenols in the Periphery and the Central Nervous System Following Oral Administration

Expression of Anthocyanins in Callus Cultures of Cranberry (Vaccinium macrocarpon Ait)

Establishment and characterization of a Satureja khuzistanica Jamzad (Lamiaceae) cell suspension culture: a new in vitro source of rosmarinic acid

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Enhanced anthocyanin production in grape cell cultures

Cited by 72 publications

References 33 publications

Pharmacokinetics and Tissue Distribution of14C-Labeled Grape Polyphenols in the Periphery and the Central Nervous System Following Oral Administration

Pharmacokinetics and Tissue Distribution of14C-Labeled Grape Polyphenols in the Periphery and the Central Nervous System Following Oral Administration

Expression of Anthocyanins in Callus Cultures of Cranberry (Vaccinium macrocarpon Ait)

Establishment and characterization of a Satureja khuzistanica Jamzad (Lamiaceae) cell suspension culture: a new in vitro source of rosmarinic acid

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Product

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Pharmacokinetics and Tissue Distribution of¹⁴C-Labeled Grape Polyphenols in the Periphery and the Central Nervous System Following Oral Administration

Pharmacokinetics and Tissue Distribution of¹⁴C-Labeled Grape Polyphenols in the Periphery and the Central Nervous System Following Oral Administration