Establishment and characterization of a Satureja khuzistanica Jamzad (Lamiaceae) cell suspension culture: a new in vitro source of rosmarinic acid

Sahraroo, Amir; Mirjalili, Mohammad Hossein; Corchete, Purificación; Babalar, Mesbah; Moghadam, Mohammad Reza Fattahi

doi:10.1007/s10616-015-9901-x

Cited by 40 publications

(8 citation statements)

References 52 publications

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“…The growth kinetics is similar to that for Spilanthes acmella , in which the cell suspension culture reached a specific growth rate of 0.28 days −1 ; during the exponential phase, the doubling time was 2.50 days −1 , and the maximum biomass was 8.5-g L −1 at day 15 [ 32 ]. On the other hand, Satureja khuzistanica cell suspension cultures reached a maximum dry biomass of 19.7-g L −1 at 21 days, with a specific growth rate of 1.5 days −1 and a doubling time of 7.6 days −1 [ 33 ]. Similarly, cell suspension cultures of Helianthus annuus produced 12.7-g L −1 dry biomass after nine days of culture, showing a specific growth rate of 0.21 days −1 and a doubling time of 3.31 days −1 [ 34 ].…”

Section: Resultsmentioning

confidence: 99%

Establishment of a Cell Suspension Culture of Ageratina pichinchensis (Kunth) for the Improved Production of Anti-Inflammatory Compounds

Sánchez-Ramos

Álvarez

Romero-Estrada

et al. 2020

Plants

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Ageratina pichinchensis (Kunth) is a plant used in traditional Mexican medicine to treat multiple ailments. However, there have not been biotechnological studies on producing compounds in in vitro cultures. The aim of this study was to establish a cell suspension culture of A. pichinchensis, quantify the anti-inflammatory constituents 2,3-dihydrobenzofuran (2) and 3-epilupeol (3), evaluate the anti-inflammatory potential of its extracts, and perform a phytochemical analysis. Cell suspension cultures were established in a MS culture medium of 30-g L−1 sucrose, 1.0-mg L−1 α-naphthaleneacetic acid, and 0.1-mg L−1 6-furfurylaminopurine. The ethyl acetate extract of the cell culture analyzed by gas chromatography (GC) revealed that the maximum production of anti-inflammatory compounds 2 and 3 occurs on days eight and 16, respectively, improving the time and previously reported yields in callus cultures. The anti-inflammatory activity of these extracts exhibited a significant inhibition of nitric oxide (NO) production. Furthermore, a phytochemical study of the ethyl acetate (EtOAc) and methanol (MeOH) extracts from day 20 led to the identification of 17 known compounds. The structures of the compounds were assigned by an analysis of 1D and 2D NMR data and the remainder by GC–MS. This is the first report of the production of (-)-Artemesinol, (-)-Artemesinol glucoside, encecalin, and 3,5-diprenyl-acetophenone by a cell suspension culture of A. pichinchensis.

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Section: Resultsmentioning

confidence: 99%

Establishment of a Cell Suspension Culture of Ageratina pichinchensis (Kunth) for the Improved Production of Anti-Inflammatory Compounds

Sánchez-Ramos

Álvarez

Romero-Estrada

et al. 2020

Plants

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“…Alternatively, the biotechnological production of SMs through in vitro plant cell, tissue, and organ culture is an efficient and faster method that guarantees stable and controlled conditions. These in vitro techniques have been widely used for the production of several SMs, notably taxol [ 31 , 32 , 33 ], podophyllotoxin [ 34 , 35 , 36 ], withanolides [ 37 ], centellosides [ 38 , 39 ], and rosmarinic acid (RA) [ 40 , 41 , 42 , 43 ], as well as for mass propagation [ 44 ], in vitro cloning [ 45 , 46 , 47 ], and polyploidization [ 48 , 49 ]. Many RA-producing biotechnological platforms have been established, based on shoots [ 50 , 51 , 52 ], callus [ 53 ], cells [ 41 , 42 , 54 ], and hairy root cultures [ 55 , 56 , 57 , 58 ] of numerous species of the Boraginaceae, Anthocerotaceae, and Lamiaceae.…”

Section: Introductionmentioning

confidence: 99%

“…In recent years, our research group has developed callus and cell suspension cultures of Satureja khuzistanica Jamzad (Lamiaceae), a rich source of RA [ 41 , 42 , 53 , 54 ], for further scaling up and commercial production of this valuable natural antioxidant. The present review is focused on the recent biotechnological and metabolic engineering achievements in the production of valuable natural antioxidants, with special emphasis on the use of S. khuzistanica in vitro cultures as a chemical RA factory.…”

Section: Introductionmentioning

confidence: 99%

Powerful Plant Antioxidants: A New Biosustainable Approach to the Production of Rosmarinic Acid

et al. 2020

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Modern lifestyle factors, such as physical inactivity, obesity, smoking, and exposure to environmental pollution, induce excessive generation of free radicals and reactive oxygen species (ROS) in the body. These by-products of oxygen metabolism play a key role in the development of various human diseases such as cancer, diabetes, heart failure, brain damage, muscle problems, premature aging, eye injuries, and a weakened immune system. Synthetic and natural antioxidants, which act as free radical scavengers, are widely used in the food and beverage industries. The toxicity and carcinogenic effects of some synthetic antioxidants have generated interest in natural alternatives, especially plant-derived polyphenols (e.g., phenolic acids, flavonoids, stilbenes, tannins, coumarins, lignins, lignans, quinines, curcuminoids, chalcones, and essential oil terpenoids). This review focuses on the well-known phenolic antioxidant rosmarinic acid (RA), an ester of caffeic acid and (R)-(+)-3-(3,4-dihydroxyphenyl) lactic acid, describing its wide distribution in thirty-nine plant families and the potential productivity of plant sources. A botanical and phytochemical description is provided of a new rich source of RA, Satureja khuzistanica Jamzad (Lamiaceae). Recently reported approaches to the biotechnological production of RA are summarized, highlighting the establishment of cell suspension cultures of S. khuzistanica as an RA chemical biofactory.

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“…Many RA‐producing biotechnological platforms have been established, including shoots ; cell suspensions ; and hairy root cultures of numerous species of the Boraginaceae, Anthocerotaceae, and Lamiaceae (reviewed in Bulgakov et al. ), including Satureja khuzistanica . Several elicitor treatments have been applied to enhance RA yields, including fungal elicitors, cuprum ions, silver ions, salicylic acid, or methyl jasmonate (MeJA) .…”

Section: Introductionmentioning

confidence: 99%

Methyl jasmonate enhanced production of rosmarinic acid in cell cultures of Satureja khuzistanica in a bioreactor

Khojasteh

Mirjalili

Palazón

et al. 2016

Engineering in Life Sciences

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The growing interest in rosmarinic acid (RA), an ester of caffeic acid and 3,4‐dihydroxyphenyl lactic acid, is due to its biological activities, which include cognitive‐enhancing effects, slowing the development of Alzheimer's disease, cancer chemoprotection, and anti‐inflammatory activity. Inspired by the challenge of meeting the growing demand for this plant secondary metabolite, we developed a biotechnological platform based on cell suspension cultures of Satureja khuzistanica. The high amounts of RA produced by this system accumulated mainly inside the cells. To further improve production, two elicitors, 100 μM methyl jasmonate (MeJA) and 40 mM cyclodextrin (CD), were tested, separately and together. MeJA increased RA productivity more than 3‐fold, the elicited cultures achieving an RA production of 3.9 g L−1 without affecting biomass productivity. CD did not have a clear effect on RA production, and under the combined treatment of MeJA + CD only a small amount of RA was released to the medium. When the cell culture was transferred from a shake flask to a wave‐mixed bioreactor, a maximum RA production of 3.1 g L−1 and biomass productivity of 18.7 g L−1 d−1 was achieved under MeJA elicitation, demonstrating the suitability of S. khuzistanica cell suspensions for the biotechnological production of this bioactive plant secondary metabolite.

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Establishment and characterization of a Satureja khuzistanica Jamzad (Lamiaceae) cell suspension culture: a new in vitro source of rosmarinic acid

Cited by 40 publications

References 52 publications

Establishment of a Cell Suspension Culture of Ageratina pichinchensis (Kunth) for the Improved Production of Anti-Inflammatory Compounds

Establishment of a Cell Suspension Culture of Ageratina pichinchensis (Kunth) for the Improved Production of Anti-Inflammatory Compounds

Powerful Plant Antioxidants: A New Biosustainable Approach to the Production of Rosmarinic Acid

Methyl jasmonate enhanced production of rosmarinic acid in cell cultures of Satureja khuzistanica in a bioreactor

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