Engineering silica-polymer hybrid nanosystems for dual drug and gene delivery

Cordeiro, Rosemeyre A.; Mendonça, Patrícia V.; Coelho, Jorge F. J.; Faneca, Henrique

doi:10.1016/j.bioadv.2022.212742

Cited by 5 publications

(5 citation statements)

References 30 publications

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“…The loading of the anticancer drug Epirubicin (Epi) into the nanoparticles was achieved through an adapted procedure [ 3 , 24 ]. Typically, 5 mg of nanoparticles was suspended in 1 mL of Epi (2 mg/mL), and the obtained suspension was stirred at room temperature in the dark for 24 h. The resulting suspension was centrifuged at 4000 rpm for 10 min (Rotofix 32 A Centrifuge (Hettich, Tuttlingen, Germany)) and washed with ultrapure water twice to remove the unloaded drug.…”

Section: Methodsmentioning

confidence: 99%

“…Mesoporous silica nanoparticle (MSN)-based systems have aroused great interest in the field of drug delivery due to numerous advantages. In fact, not only their structural properties (large surface area and pore volume) allow for a good loading capacity and easy drug encapsulation; however, their simple, scalable, and cost-effective production are attractive features in the development of a drug delivery system, namely for application in cancer treatment [ 1 , 2 , 3 ]. Their easy synthetic and post-synthetic procedures allow them to incorporate specific characteristics, such as stimuli-responsive drug release behavior, or functionalization with molecules of interest (such as ligands) to direct them to target cells [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

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Triantennary GalNAc-Functionalized Multi-Responsive Mesoporous Silica Nanoparticles for Drug Delivery Targeted at Asialoglycoprotein Receptor

Cordeiro

Carvalho

Durães

et al. 2022

IJMS

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In recent years, mesoporous silica particles have been revealed as promising drug delivery systems combining high drug loading capacity, excellent biocompatibility, and easy and affordable synthetic and post-synthetic procedures. In fact, the straightforward functionalization approaches of these particles allow their conjugation with targeting moieties in order to surpass one of the major challenges in drug administration, the absence of targeting ability of free drugs that reduces their therapeutic efficacy and causes undesired side effects. In this context, the main goal of this work was to develop a new targeted mesoporous silica nanoparticle formulation with the capability to specifically and efficiently deliver an anticancer drug to hepatocellular carcinoma (HCC) cells. To this purpose, and as proof of concept, we developed redox-responsive mesoporous silica nanoparticles functionalized with the targeting ligand triantennary N-acetylgalactosamine (GalNAc) cluster, which has high affinity to asialoglycoprotein receptors overexpressed in HCC cells, and loaded them with epirubicin, an anthracycline drug. The produced nanocarrier exhibits suitable physicochemical properties for drug delivery, high drug loading capacity, high biocompatibility, and targeting ability to HCC cells, revealing its biopharmaceutical potential as a targeted drug carrier for therapeutic applications in liver diseases.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Triantennary GalNAc-Functionalized Multi-Responsive Mesoporous Silica Nanoparticles for Drug Delivery Targeted at Asialoglycoprotein Receptor

Cordeiro

Carvalho

Durães

et al. 2022

IJMS

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“…After 1 h (SCC-9) or 4 h (U2OS) the absorbance of the cell medium was measured at 570 and 600 nm. The cell viability was calculated as described previously [ 17 ].…”

Section: Methodsmentioning

confidence: 99%

Novel Non-Viral Vectors Based on Pluronic® F68PEI with Application in Oncology Field

et al. 2022

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Copolymers composed of low-molecular-weight polyethylenimine (PEI) and amphiphilic Pluronics® are safe and efficient non-viral vectors for pDNA transfection. A variety of Pluronic® properties provides a base for tailoring transfection efficacy in combination with the unique biological activity of this polymer group. In this study, we describe the preparation of new copolymers based on hydrophilic Pluronic® F68 and PEI (F68PEI). F68PEI polyplexes obtained by doping with free F68 (1:2 and 1:5 w/w) allowed for fine-tuning of physicochemical properties and transfection activity, demonstrating improved in vitro transfection of the human bone osteosarcoma epithelial (U2OS) and oral squamous cell carcinoma (SCC-9) cells when compared to the parent formulation, F68PEI. Although all tested systems condensed pDNA at varying polymer/DNA charge ratios (N/P, 5/1–100/1), the addition of free F68 (1:5 w/w) resulted in the formation of smaller polyplexes (<200 nm). Analysis of polyplex properties by transmission electron microscopy and dynamic light scattering revealed varied polyplex morphology. Transfection potential was also found to be cell-dependent and significantly higher in SCC-9 cells compared to the control bPEI25k cells, as especially evident at higher N/P ratios (>25). The observed selectivity towards transfection of SSC-9 cells might represent a base for further optimization of a cell-specific transfection vehicle.

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“…2,259 In this case, attempts have been made to address this issue by coating the MSNs surface with polymers and lipid bilayers, or by introducing environmentally responsive factors. [260][261][262][263][264] Surface-functionalized modifications of MSNs not only offer unique advantages in terms of improved biosafety, long circulation and targeting ability, but can also be used to construct intelligent stimuli-responsive drug delivery systems. [265][266][267][268][269] Once they reach the lesion sites, the spatial and temporal controlled release of drugs in response to stimuli such as pH, 270,271 temperature, 272,273 light [274][275][276] and ultrasound 277,278 can be achieved.…”

Section: Surface Functionalizationmentioning

confidence: 99%

Multifunctional mesoporous silica nanoparticles for biomedical applications

Xu,

Li,

Shi

et al. 2023

Sig Transduct Target Ther

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Mesoporous silica nanoparticles (MSNs) are recognized as a prime example of nanotechnology applied in the biomedical field, due to their easily tunable structure and composition, diverse surface functionalization properties, and excellent biocompatibility. Over the past two decades, researchers have developed a wide variety of MSNs-based nanoplatforms through careful design and controlled preparation techniques, demonstrating their adaptability to various biomedical application scenarios. With the continuous breakthroughs of MSNs in the fields of biosensing, disease diagnosis and treatment, tissue engineering, etc., MSNs are gradually moving from basic research to clinical trials. In this review, we provide a detailed summary of MSNs in the biomedical field, beginning with a comprehensive overview of their development history. We then discuss the types of MSNs-based nanostructured architectures, as well as the classification of MSNs-based nanocomposites according to the elements existed in various inorganic functional components. Subsequently, we summarize the primary purposes of surface-functionalized modifications of MSNs. In the following, we discuss the biomedical applications of MSNs, and highlight the MSNs-based targeted therapeutic modalities currently developed. Given the importance of clinical translation, we also summarize the progress of MSNs in clinical trials. Finally, we take a perspective on the future direction and remaining challenges of MSNs in the biomedical field.

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Engineering silica-polymer hybrid nanosystems for dual drug and gene delivery

Cited by 5 publications

References 30 publications

Triantennary GalNAc-Functionalized Multi-Responsive Mesoporous Silica Nanoparticles for Drug Delivery Targeted at Asialoglycoprotein Receptor

Triantennary GalNAc-Functionalized Multi-Responsive Mesoporous Silica Nanoparticles for Drug Delivery Targeted at Asialoglycoprotein Receptor

Novel Non-Viral Vectors Based on Pluronic® F68PEI with Application in Oncology Field

Multifunctional mesoporous silica nanoparticles for biomedical applications

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