Engineering of a chitosanase fused to a carbohydrate-binding module for continuous production of desirable chitooligosaccharides

Zhou, Jianli; Harindintwali, Jean Damascene; Yang, Wenhua; Han, Minghai; Deng, Bin; Luan, Hao; Zhang, Wenshuai; Liu, Xiaobo; Yu, Xiaobin

doi:10.1016/j.carbpol.2021.118609

Cited by 27 publications

(19 citation statements)

References 55 publications

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“…Several nonspecific enzymes such as protease, lipase, cellulase, hemicellulase, lysozyme, pectinase, and α-amylase can also be applied this process [ 39 ]. Meanwhile, the development and application of immobilized enzyme technology reduce the production cost and achieve the continuous production of COS [ 40 ]. The schematic diagram of the process for producing chitosan and COS from chitin is shown in Figure 1 .…”

Section: Production Physicochemical Properties and Applications Of Ch...mentioning

confidence: 99%

Chitosan and Chitooligosaccharide: The Promising Non-Plant-Derived Prebiotics with Multiple Biological Activities

Guan

Feng

2022

IJMS

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Biodegradable chitin is the second-most abundant natural polysaccharide, widely existing in the exoskeletons of crabs, shrimps, insects, and the cell walls of fungi. Chitosan and chitooligosaccharide (COS, also named chitosan oligosaccharide) are the two most important deacetylated derivatives of chitin. Compared with chitin, chitosan and COS not only have more satisfactory physicochemical properties but also exhibit additional biological activities, which cause them to be widely applied in the fields of food, medicine, and agriculture. Additionally, due to their significant ability to improve gut microbiota, chitosan and COS are deemed prospective prebiotics. Here, we introduced the production, physicochemical properties, applications, and pharmacokinetic characteristics of chitosan and COS. Furthermore, we summarized the latest research on their antioxidant, anti-inflammatory, and antimicrobial activities. Research progress on the prebiotic functions of chitosan and COS is particularly reviewed. We creatively analyzed and discussed the mechanisms and correlations underlying these activities of chitosan and COS and their physicochemical properties. Our work enriched people’s understanding of these non-plant-derived prebiotics. Based on this review, the future directions of research on chitosan and COS are explored. Collectively, optimizing the production technology of chitin derivatives and enriching understanding of their biological functions will shed more light on their capability to improve human health.

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Section: Production Physicochemical Properties and Applications Of Ch...mentioning

confidence: 99%

Chitosan and Chitooligosaccharide: The Promising Non-Plant-Derived Prebiotics with Multiple Biological Activities

Guan

Feng

2022

IJMS

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“…This also demonstrates that P. pastoris is an ideal host for the construction of synthetic pathways towards complex natural products. 58,59 Moreover, the standardized operating procedure and high-density cultivation of P. pastoris with the C1 compound methanol as the carbon source also contributes to its wide industrial applications. 60 To increase the productivity and sulfation level of the bioengineered heparin, near future studies should be focused on optimizing the biosynthesis of precursors UDP-GlcA and UDP-GlcNAc, 33 regenerating the sulfate group donor PAPS, 43 and increasing the catalytic efficiencies of these sulfotransferases in P. pastoris .…”

Section: Resultsmentioning

confidence: 99%

Synthesis of bioengineered heparin by recombinant yeast Pichia pastoris

et al. 2022

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“…The reaction was incubated at 50 °C for 20 min and stopped by boiling for 10 min . The hydrolysate was qualitatively analyzed by thin-layer chromatography (TLC) …”

Section: Methodsmentioning

confidence: 99%

Establishment of a Novel Cell Surface Display Platform Based on Natural “Chitosan Beads” of Yeast Spores

Wang

et al. 2022

J. Agric. Food Chem.

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Cell surface display technology, which expresses and anchors proteins on the surface of microbial cells, has broad application prospects in many fields, such as protein library screening, biocatalysis, and biosensor development. However, traditional cell surface display systems have disadvantages: the molecular weight of phage display proteins cannot be too large; bacterial display lacks the post-translational modification process for eukaryotic proteins; yeast display is prone to excessive protein glycosylation and misfolding of multisubunit proteins; and the compatibility of Bacillus subtilis spore display needs to be further improved. Therefore, it is extremely valuable to develop an efficient surface display platform with strong universality and stress resistance properties. Although yeast surface display systems have been extensively investigated, the establishment of a surface display platform using yeast spores has rarely been reported. In this study, a novel cell surface display platform based on natural "chitosan beads" of yeast spores was developed. The target protein in fusion with the chitosan affinity protein (CAP) exhibited strong binding capability with "chitosan beads" of yeast spores in vitro and in vivo. Moreover, this protein display system showed highly preferable enzymatic properties and stability. As an example, the displayed LXYL-P1-2-CAP demonstrated high thermostability and reusability (60% of the initial activity after seven cycles of reuse), high storage stability (75% of original activity after 8 weeks), and excellent tolerance to a concentration up to 75% (v/v) organic reagents. To prove the practicability of this surface display system, the semisynthesis of paclitaxel intermediate was demonstrated and its highest conversion rate was 92% using 0.25 mM substrate. This study provides a novel and useful platform for the surface display of proteins, especially for multimeric macromolecular proteins of eukaryotic origin.

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Engineering of a chitosanase fused to a carbohydrate-binding module for continuous production of desirable chitooligosaccharides

Cited by 27 publications

References 55 publications

Chitosan and Chitooligosaccharide: The Promising Non-Plant-Derived Prebiotics with Multiple Biological Activities

Chitosan and Chitooligosaccharide: The Promising Non-Plant-Derived Prebiotics with Multiple Biological Activities

Synthesis of bioengineered heparin by recombinant yeast Pichia pastoris

Establishment of a Novel Cell Surface Display Platform Based on Natural “Chitosan Beads” of Yeast Spores

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