Engineering calcium peroxide based oxygen generating scaffolds for tissue survival

Suvarnapathaki, Sanika; Nguyen, Michelle; Goulopoulos, Anastasia; Lantigua, Darlin; Camci‐Unal, Gulden

doi:10.1039/d0bm02048f

Cited by 28 publications

(23 citation statements)

References 55 publications

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“…A major challenge in the use of CaO 2 NPs as an oxygen-generating material is that they produce H 2 O 2 as a reaction intermediate by reacting with water (eq ). ,, In the absence of catalysts, the decomposition of H 2 O 2 to release oxygen is hampered, which may lead to cellular damage . Additionally, the decomposition of H 2 O 2 should also be controlled to provide a sustained oxygen release as well as to avoid hyperoxia, which may further lead to cellular damage .…”

Section: Resultsmentioning

confidence: 99%

“…The Alamar Blue test further indicated that, although a slight cell viability decrease was observed for PLA/7.5_CaO 2 sample, all membranes were not cytotoxic (Figure F). It is important to note that although calcium peroxide decomposes exothermically in water (which, in turn, could lead to a decrease in cell viability , ), its incorporation into a polymeric matrix may contribute to the dissipation of generated heat. Besides, the PLA hydrophobic matrix reduces the water diffusivity into the fiber’s structure and, consequently, the CaO 2 NP decomposition rate, which, in turn, decreases the heat release rate, not affecting cell viability.…”

Section: Resultsmentioning

confidence: 99%

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Electrospun Composite Bead-on-String Nanofibers Containing CaO₂ Nanoparticles and MnO₂ Nanosheets as Oxygen-Release Systems for Biomedical Applications

Santos

Dias

Surur

et al. 2022

ACS Appl. Nano Mater.

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Oxygen-generating biomaterials have the potential to improve tissue engineering and regenerative therapeutic strategies. However, the development of such materials capable of controlling the local partial pressure of oxygen (pO2) in the long term is still a major challenge. Here we report nanostructured composite membranes comprising electrospun fibers exhibiting a bead-on-string structure as a controlled oxygen-release system for periodontitis treatment. For this, calcium peroxide nanoparticles (CaO2 NPs) and manganese dioxide nanosheets (MnO2 NSs) were incorporated into the structure of hydrophobic electrospun poly (lactic acid) (PLA)-based nanofibers. We use CaO2 NPs as hydrogen peroxide (H2O2)-generating precursors when exposed to water, while MnO2 NSs were applied as a nanozyme to catalyze the decomposition of H2O2 to the final oxygen product. Our results revealed that the beads on the fibrous structure acted as reservoirs of CaO2 NPs and MnO2 NSs. Moreover, the composite membranes provided sustained oxygen release over 7 days, where levels were modulated by the CaO2 NP content. Such constructs exhibited suitable physicochemical properties and antimicrobial activities against some bacteria (e.g., Porphyromonas gingivalis and Treponema denticola) typically associated with aggressive and chronic periodontitis. In vitro studies also revealed that the membranes were not cytotoxic toward human oral keratinocyte (Nok-si) cells as well as enhanced the cell viability when high content of CaO2 NP and MnO2 NS were incorporated into the fiber’s structure. Taken together, our results demonstrate that the nanostructured composite membranes show potential to be employed as oxygen-release platforms for periodontal tissue regeneration.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Electrospun Composite Bead-on-String Nanofibers Containing CaO₂ Nanoparticles and MnO₂ Nanosheets as Oxygen-Release Systems for Biomedical Applications

Santos

Dias

Surur

et al. 2022

ACS Appl. Nano Mater.

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“…Even though the level of expression of catalase mRNA is not changed during wound healing 71 , the protein level of catalase is decreased 72 . For example, catalase concentration is reported to be in the range of 50 – 100 U/mL in the oxygen generating gelatin section 73 or 1 mg/mL in the matrix of polycaprolactone (PCL) 74 . In the mixture of CPO, catalase and SLS or TLS, the dissociation of dissociation of H 2 O 2 is predominantly facilitated by catalase and partly by TLS and to less extent by SLS ( Figure S5B ).…”

Section: Discussionmentioning

confidence: 99%

Engineering Antioxidant and Oxygen-Releasing Lignin Composites to Promote Wound Healing

Balaji

Short

Farouk

et al. 2022

Preprint

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The application of engineered biomaterials for wound healing has been pursued since the beginning of tissue engineering. Here, we attempt to apply functionalized lignosulfonates to confer antioxidation to tissue microenvironments and to deliver oxygen to accelerate vascularization and healing responses without causing inflammatory responses. The results from fibrosis array shows that thiolated lignosulfonate in methacrylated gelatin can effectively attenuate fibrotic responses of human dermal fibroblasts. Elemental analysis of oxygen releasing nanoparticles shows the positive incorporation of calcium peroxide. Composites including nanoparticles of lignosulfonate and calcium peroxide release around 0.05% oxygen per day at least for 7 days. Stiffness can be precisely modulated to avoid adverse inflammatory responses. Injection of lignin composites with oxygen generation nanoparticles enhanced the formation of blood vessels and promoted infiltration of alpha-smooth muscle actin+ fibroblasts over 7 days. At 30 days after surgery, the lignin composite with oxygen generating nanoparticles remodels the collagen architecture resembling to the reticular pattern of normal collagen and leave minimal scars. Thus, our study shows the potential of functionalized lignosulfonate for wound healing applications requiring balanced antioxidation and controlled release of oxygen.

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“…Sodium, calcium and magnesium peroxides are the most used solid inorganic peroxides. Hydrolysis is the primary mechanism for oxygen release when these nano/microparticles interact with water, as shown in these equations [ 5 , 19 , 22 ]:…”

Section: Advances In Oxygen Delivery From Biomaterials For Cartilage ...mentioning

confidence: 99%

3D Bioprinting of Smart Oxygen-Releasing Cartilage Scaffolds

Montesdeoca

Stocco

Marciano

et al. 2022

JFB

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Three-dimensional bioprinting is a powerful technique for manufacturing improved engineered tissues. Three-dimensional bioprinted hydrogels have significantly advanced the medical field to repair cartilage tissue, allowing for such constructs to be loaded with different components, such as cells, nanoparticles, and/or drugs. Cartilage, as an avascular tissue, presents extreme difficulty in self-repair when it has been damaged. In this way, hydrogels with optimal chemical and physical properties have been researched to respond to external stimuli and release various bioactive agents to further promote a desired tissue response. For instance, methacryloyl gelatin (GelMA) is a type of modified hydrogel that allows for the encapsulation of cells, as well as oxygen-releasing nanoparticles that, in the presence of an aqueous medium and through controlled porosity and swelling, allow for internal and external environmental exchanges. This review explores the 3D bioprinting of hydrogels, with a particular focus on GelMA hydrogels, to repair cartilage tissue. Recent advances and future perspectives are described.

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Engineering calcium peroxide based oxygen generating scaffolds for tissue survival

Abstract: Implanted tissue constructs require 4 to 5 weeks for homogeneous vascularization. We show assemblies of oxygen-generating scaffolds to substitute vasculature and assist cell viability and function through oxygen release up to 35 days under hypoxia.

Cited by 28 publications

References 55 publications

Electrospun Composite Bead-on-String Nanofibers Containing CaO₂ Nanoparticles and MnO₂ Nanosheets as Oxygen-Release Systems for Biomedical Applications

Electrospun Composite Bead-on-String Nanofibers Containing CaO₂ Nanoparticles and MnO₂ Nanosheets as Oxygen-Release Systems for Biomedical Applications

Engineering Antioxidant and Oxygen-Releasing Lignin Composites to Promote Wound Healing

3D Bioprinting of Smart Oxygen-Releasing Cartilage Scaffolds

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Engineering calcium peroxide based oxygen generating scaffolds for tissue survival

Abstract: Implanted tissue constructs require 4 to 5 weeks for homogeneous vascularization. We show assemblies of oxygen-generating scaffolds to substitute vasculature and assist cell viability and function through oxygen release up to 35 days under hypoxia.

Cited by 28 publications

References 55 publications

Electrospun Composite Bead-on-String Nanofibers Containing CaO2 Nanoparticles and MnO2 Nanosheets as Oxygen-Release Systems for Biomedical Applications

Electrospun Composite Bead-on-String Nanofibers Containing CaO2 Nanoparticles and MnO2 Nanosheets as Oxygen-Release Systems for Biomedical Applications

Engineering Antioxidant and Oxygen-Releasing Lignin Composites to Promote Wound Healing

3D Bioprinting of Smart Oxygen-Releasing Cartilage Scaffolds

Contact Info

Product

Resources

About

Electrospun Composite Bead-on-String Nanofibers Containing CaO₂ Nanoparticles and MnO₂ Nanosheets as Oxygen-Release Systems for Biomedical Applications

Electrospun Composite Bead-on-String Nanofibers Containing CaO₂ Nanoparticles and MnO₂ Nanosheets as Oxygen-Release Systems for Biomedical Applications