3D Bioprinting of Smart Oxygen-Releasing Cartilage Scaffolds

Montesdeoca, Caterine Yesenia Carrasco; Stocco, Thiago Domingues; Marciano, Fernanda Roberta; Webster, Thomas J; Lobo, Anderson Oliveira

doi:10.3390/jfb13040252

Cited by 6 publications

(4 citation statements)

References 84 publications

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“…Materials commonly utilized to construct the scaffolds include natural polymers (such as gelatin, collagen, and chitosan-alginate), synthetic polymers (such as poly (ε-caprolactone) (PCL) and polylactic acids (PLAs), colloidal crystal poly), and bioactive ceramics (hydroxyapatite and silica) [122,123]. These materials were further used to fabricate porous scaffolds via various procedures, such as emulsion [124], electrospinning [125][126][127], solvent-casting particleleaching [127], 3D printing [128], stereolithography [129] and gas foaming [130].…”

Section: Scaffoldsmentioning

confidence: 99%

Artificial tumor matrices and bioengineered tools for tumoroid generation

Liu,

Chen,

Chang

et al. 2024

Biofabrication

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The tumor microenvironment (TME) is critical for tumor growth and metastasis. The TME contains cancer-associated cells, tumor matrix, and tumor secretory factors. The fabrication of artificial tumors, so-called tumoroids, is of great significance for understanding tumorigenesis and clinical cancer therapy. The assembly of multiple tumor cells and matrix components through interdisciplinary techniques is necessary to prepare various tumoroids. This article discusses current methods for constructing tumoroids (tumor tissue slices and tumor cell co-culture) for pre-clinical use. This article focuses on the artificial matrix materials (natural and synthetic materials) and biofabrication techniques (cell assembly, bioengineered tools, bioprinting, and microfluidic devices) used in tumoroids. This article also points out the shortcomings of current tumoroids and potential solutions. This article aims to promote the next-generation tumoroids and their potential in basic research and clinical application.

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Section: Scaffoldsmentioning

confidence: 99%

Artificial tumor matrices and bioengineered tools for tumoroid generation

Liu,

Chen,

Chang

et al. 2024

Biofabrication

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“…Um enfoque de destaque na bioimpressão 3D direcionada à cartilagem articular é a introdução de micro ou nanopartículas projetadas para liberar oxigênio dentro da estrutura impressa, já que um dos desafios predominantes na engenharia de tecidos é a falta de oxigenação adequada no interior das estruturas, levando à morte celular 36,37 . Estudos aprofundados nessa direção foram recentemente revisados e publicado por nosso grupo de pesquisa 38 .…”

Section: Cartilagem Articularunclassified

Bioimpressão 3D aplicada à engenharia de tecidos

Stocco,

Trindade da Silva,

Gonçalves Tsumura

et al. 2023

VICV

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O campo da engenharia de tecidos, uma área interdisciplinar que visa criar tecidos artificiais funcionais imitando o tecido nativo para reparar e/ou substituir tecidos e órgãos danificados, evoluiu significativamente nos últimos anos. No entanto, as abordagens tradicionais de biofabricação tiveram taxas de sucesso limitadas na construção de tecidos complexos. Nesse cenário, a bioimpressão 3D se destacou como uma tecnologia promissora para superar essas limitações. O princípio fundamental da bioimpressão 3D é a capacidade de produzir estruturas biológicas personalizadas combinando células vivas, materiais biocompatíveis e tecnologia de impressão 3D. O objetivo é fornecer soluções médicas para doenças, lesões e condições que afetam o sistema de tecidos, oferecendo uma alternativa à escassez de doadores de órgãos e ajudando a resolver problemas relacionados à rejeição. Neste capítulo, buscamos oferecer um entendimento geral sobre a tecnologia de bioimpressão 3D, abordando os conceitos fundamentais de cada etapa do processo. Em seguida, concentramo-nos nas técnicas de bioimpressão 3D mais utilizadas na fabricação de implantes de tecido e regenerativos. Por fim, apresentamos exemplos das principais aplicações da tecnologia de bioimpressão 3D na engenharia de tecidos, bem como as principais limitações e considerações finais desta estratégia de biofabricação em ascensão.

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“…However, PHD2 is less active during hypoxia, which leads to cytosolic accumulation and nuclear translocation of HIF-1α, where, together with transcriptional cofactors, it activates the expression of its target genes in the HIF complex, such as BNIP3, an essential molecule for mitophagy ( 22 , 23 ). It has been shown to stimulate chondrogenesis of progenitor or stem cells by decreasing the oxygen pressure locally within a biomaterial using various molecules or simply by limiting oxygen diffusion ( 24 , 25 ).…”

Section: Introductionmentioning

confidence: 99%

UCHL1 alleviates apoptosis in chondrocytes via upregulation of HIF‑1α‑mediated mitophagy

Yan,

Shi,

et al. 2023

Int J Mol Med

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Stem cell-based tissue engineering has shown significant potential for rapid restoration of injured cartilage tissues. Stem cells frequently undergo apoptosis because of the prevalence of oxidative stress and inflammation in the microenvironment at the sites of injury. Our previous study demonstrated that stabilization of hypoxia-inducible factor 1α (HIF-1α) is key to resisting apoptosis in chondrocytes. Recently, it was reported that Ubiquitin C-terminal hydrolase L1 (UCHL1) can stabilize HIF-1α by abrogating the ubiquitination process. However, the effect of UCHL1 on apoptosis in chondrocytes remains unclear. Herein, adipose-derived stem cells were differentiated into chondrocytes. Next, the CRISPR activation (CRISPRa) system, LDN-57444 (LDM; a specific inhibitor for UCHL1), KC7F2 (a specific inhibitor for HIF-1α), and 3-methyladenine (a specific inhibitor for mitophagy) were used to activate or block UCHL1, HIF-1α, and mitophagy. Mitophagy, apoptosis, and mitochondrial function in chondrocytes were detected using immunofluorescence, TUNEL staining, and flow cytometry. Moreover, the oxygen consumption rate of chondrocytes was measured using the Seahorse XF 96 Extracellular Flux Analyzer. UCHL1 expression was increased in hypoxia, which in turn regulated mitophagy and apoptosis in the chondrocytes. Further studies revealed that UCHL1 mediated hypoxia-regulated mitophagy in the chondrocytes. The CRISPRa module was utilized to activate UCHL1 effectively for 7 days; endogenous activation of UCHL1 accelerated mitophagy, inhibited apoptosis, and maintained mitochondrial function in the chondrocytes, which was mediated by HIF-1α. Taken together, UCHL1 could block apoptosis in chondrocytes via upregulation of HIF-1α-mediated mitophagy and maintain mitochondrial function. These results indicate the potential of UCHL1 activation using the CRISPRa system for the regeneration of cartilage tissue.

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3D Bioprinting of Smart Oxygen-Releasing Cartilage Scaffolds

Cited by 6 publications

References 84 publications

Artificial tumor matrices and bioengineered tools for tumoroid generation

Artificial tumor matrices and bioengineered tools for tumoroid generation

Bioimpressão 3D aplicada à engenharia de tecidos

UCHL1 alleviates apoptosis in chondrocytes via upregulation of HIF‑1α‑mediated mitophagy

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