Engineered composite fascia for stem cell therapy in tissue repair applications

Ayala, Perla; Caves, Jeffrey M.; Dai, Erbin; Siraj, Layla; Liu, Liying; Chaudhuri, Ovijit; Haller, Carolyn A.; Mooney, David J.; Chaikof, Elliot L.

doi:10.1016/j.actbio.2015.08.012

Cited by 25 publications

(21 citation statements)

References 48 publications

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“…Suture retention strength testing was done as previously described . Briefly, Prolene 4‐0 sutures were passed through 5 mm square samples, 2.5 mm from the patch edge and the suture fastened to the actuating arm of the DMTA pulled at a rate of 1 mm/s.…”

Section: Methodsmentioning

confidence: 99%

“…Alginate gel was prepared, as previously described . Briefly, medium viscosity ultrapure grade (MVG) alginate (M/G: 40/60, ProNova Biomedical) was used as the high molecular weight (HMW, 2.65 × 10 5 g/mol) gel.…”

Section: Methodsmentioning

confidence: 99%

“…Collagen sheets and constructs were fabricated, as previously described. 23 To prepare collagen gels, isolated porcine type I collagen was dissolved in 10 mM HCl (2.5 mg/mL) and neutralized with a phosphate buffer (4.14 mg/mL monobasic sodium phosphate, 12.1 mg/ml dibasic sodium phosphate, 6.86 mg/mL n-tris(hydroxymethyl)methyl-2-aminoethane sulfonic acid sodium salt, 7.89 mg/mL sodium chloride, pH 8.0) at 48C in a rectangular mold (10 3 7 3 0.8 cm) for 24 h. Porcine collagen molded gels were then allowed to warm to room temperature for 2 h. After an additional 24 h incubation at 378C, gels were rinsed in deionized water, removed, and placed on a glass substrate where they were allowed to dry overnight to form a collagen film. Collagen films were cut into rectangles (25 3 15 mm 2 ) with a CO 2 laser (Universal Laser Systems, Scottsdale, AZ) and rehydrated for 30 min in ddH 2 O.…”

Section: Fabrication Of Porcine Collagen Sheets and Multilayer Collagmentioning

confidence: 99%

“…Innovative approaches of developing collagen‐based materials will provide new opportunities to design and fabricate synthetic ECM constructs for improved clinical outcomes. We have developed high‐density collagen composite constructs with tunable strength and flexibility, and engineered for the delivery of cells, chemokines and growth factors . In this report, collagen type I was extracted from porcine skin obtained from a US Food and Drug Administration (FDA) certified tissue provider.…”

Section: Introductionmentioning

confidence: 99%

“…We have developed high-density collagen composite constructs with tunable strength and flexibility, and engineered for the delivery of cells, chemokines and growth factors. 23 In this report, collagen type I was extracted from porcine skin obtained from a US Food and Drug Administration (FDA) certified tissue provider. The collagen was fashioned into sheets of collagen type 1 fibrils that were subsequently physically laminated and micropatterned.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of a bioengineered construct for tissue engineering applications

et al. 2017

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Effective biomaterial options for tissue repair and regeneration are limited. Current biologic meshes are derived from different tissue sources and are generally sold as decellularized tissues. This work evaluated two collagen based bioengineered constructs and a commercial product in a model of abdominal full thickness defect repair. To prepare the bioengineered construct, collagen type 1 from porcine skin was isolated using an acid solubilization method. After purification, the collagen was formed into collagen sheets that were physically bonded to form a mechanically robust construct that was subsequently laser micropatterned with pores as a means to promote tissue integration (collagen only construct). A second engineered construct consisted of the aforementioned collagen construct embedded in an RGD-functionalized alginate gel that serves as a bioactive interface (collagen-alginate construct). The commercial product is a biologic mesh derived from bovine pericardium (Veritas ). We observed enhanced vascularization in the midportion of the engineered collagen-alginate construct 2 weeks after implantation. Overall, the performance of the bioengineered constructs was similar to that of the commercial product with comparable integration strength at 8 weeks. Bioengineered constructs derived from monomeric collagen demonstrate promise for a variety of load bearing applications in tissue engineering. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2345-2354, 2018.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Fabrication Of Porcine Collagen Sheets and Multilayer Collagmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluation of a bioengineered construct for tissue engineering applications

et al. 2017

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Developing a Clinically Relevant Tissue Engineered Heart Valve—A Review of Current Approaches

Nachlas

Davis

2017

Adv Healthcare Materials

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Tissue engineered heart valves (TEHVs) have the potential to address the shortcomings of current implants through the combination of cells and bioactive biomaterials that promote growth and proper mechanical function in physiological conditions. The ideal TEHV should be anti-thrombogenic, biocompatible, durable, and resistant to calcification, and should exhibit a physiological hemodynamic profile. In addition, TEHVs may possess the capability to integrate and grow with somatic growth, eliminating the need for multiple surgeries children must undergo. Thus, this review assesses clinically available heart valve prostheses, outlines the design criteria for developing a heart valve, and evaluates three types of biomaterials (decellularized, natural, and synthetic) for tissue engineering heart valves. While significant progress has been made in biomaterials and fabrication techniques, a viable tissue engineered heart valve has yet to be translated into a clinical product. Thus, current strategies and future perspectives are also discussed to facilitate the development of new approaches and considerations for heart valve tissue engineering.

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Novel In Situ Gelling Hydrogels Loaded with Recombinant Collagen Peptide Microspheres as a Slow‐Release System Induce Ectopic Bone Formation

Fahmy-Garcia

Mumcuoglu

Miguel

et al. 2018

Adv Healthcare Materials

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New solutions for large bone defect repair are needed. Here, in situ gelling slow release systems for bone induction are assessed. Collagen-I based Recombinant Peptide (RCP) microspheres (MSs) are produced and used as a carrier for bone morphogenetic protein 2 (BMP-2). The RCP-MSs are dispersed in three hydrogels: high mannuronate (SLM) alginate, high guluronate (SLG) alginate, and thermoresponsive hyaluronan derivative (HApN). HApN+RCP-MS forms a gel structure at 32 ºC or above, while SLM+RCP-MS and SLG+RCP-MS respond to shear stress displaying thixotropic behavior. Alginate formulations show sustained release of BMP-2, while there is minimal release from HApN. These formulations are injected subcutaneously in rats. SLM+RCP-MS and SLG+RCP-MS loaded with BMP-2 induce ectopic bone formation as revealed by X-ray tomography and histology, whereas HApN+RCP-MS do not. Vascularization occurs within all the formulations studied and is significantly higher in SLG+MS and HApN+RCP-MS than in SLM+RCP-MS. Inflammation (based on macrophage subset staining) decreases over time in both alginate groups, but increases in the HApN+RCP-MS condition. It is shown that a balance between inflammatory cell infiltration, BMP-2 release, and vascularization, achieved in the SLG+RCP-MS alginate condition, is optimal for the induction of de novo bone formation.

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Engineered composite fascia for stem cell therapy in tissue repair applications

Cited by 25 publications

References 48 publications

Evaluation of a bioengineered construct for tissue engineering applications

Evaluation of a bioengineered construct for tissue engineering applications

Developing a Clinically Relevant Tissue Engineered Heart Valve—A Review of Current Approaches

Novel In Situ Gelling Hydrogels Loaded with Recombinant Collagen Peptide Microspheres as a Slow‐Release System Induce Ectopic Bone Formation

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