Evaluation of a bioengineered construct for tissue engineering applications

Abstract

Effective biomaterial options for tissue repair and regeneration are limited. Current biologic meshes are derived from different tissue sources and are generally sold as decellularized tissues. This work evaluated two collagen based bioengineered constructs and a commercial product in a model of abdominal full thickness defect repair. To prepare the bioengineered construct, collagen type 1 from porcine skin was isolated using an acid solubilization method. After purification, the collagen was formed into collagen sheets that were physically bonded to form a mechanically robust construct that was subsequently laser micropatterned with pores as a means to promote tissue integration (collagen only construct). A second engineered construct consisted of the aforementioned collagen construct embedded in an RGD-functionalized alginate gel that serves as a bioactive interface (collagen-alginate construct). The commercial product is a biologic mesh derived from bovine pericardium (Veritas ). We observed enhanced vascularization in the midportion of the engineered collagen-alginate construct 2 weeks after implantation. Overall, the performance of the bioengineered constructs was similar to that of the commercial product with comparable integration strength at 8 weeks. Bioengineered constructs derived from monomeric collagen demonstrate promise for a variety of load bearing applications in tissue engineering. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2345-2354, 2018.

Evaluating the effects of vacuum on the microstructure and biocompatibility of bovine decellularized pericardium

“…So decellularized tissues are a crucial material in tissue engineering to regenerate demolished tissues as well as bovine pericardium with some medical applications (Chang, Tsai, Liang, & Sung, 2002; Li et al, 2018). Veritas Collagen Matrix ® , Tutopatch ® , Peri‐Guard ® , Collagen Matrix ® , and integra ® are the examples of commercialized pericardial scaffolds (Ayala et al, 2018; Khan et al, 2010; Smart, Marshall, & Daniels, 2012; Wiegmann et al, 2010). All methods for decellularization of tissue to produce scaffolds require a balance in the preservation of normal ECM structure as well as the removal of cellular remains because cell debris such as DNA, plasma membrane, mitochondria, and plasma cell residuals could irritate the immune system and cause inflammation thereby delaying reconstruction (Keane et al, 2015).…”

Evaluation of vacuum washing in the removal of SDS from decellularized bovine pericardium: method and device description

“…up to now, several types of decellularized bovine pericard scaffolds have been used in tissue engineering to repair certain defects [1]. Veritas Collagen Matrix®, a kind of a commercial scaffold, is made from bovine pericardium for reconstruction and renewal of the ventricle and pelvic defects [2]. The bovine pericardium has also been used as a commercial product called Tutopatch®, employed in the repair of abdominal hernias.…”

Vascular Tissue Engineering: Progress, Challenges, and Clinical Promise

“…The idea of using non-human tissues has appealed to the tissue engineering field due to their availability compared to human tissues. However, severe and sometimes fatal immune responses to xenogeneic cells and materials such as galactose-alpha-1,3-galactose (alpha-gal) has significantly hindered progress (Ayala et al, 2017; Galili, 2005). Advances in decellularization techniques have reduced these responses, but complete removal of immunogenic foreign biomolecules still remains as a challenge (Shirakigawa and Ijima, 2017).…”