Engineered Bacteria Based Biosensors for Monitoring Bioavailable Heavy Metals

Leth, Suzanne; Maltoni, Susanna; Šimkus, Remigijus; Mattìasson, Bo; Corbisier, Philippe; Klimant, Ingo; Wolfbeis, Otto S.; Csöregi, Elisabeth

doi:10.1002/1521-4109(200201)14:1<35::aid-elan35>3.0.co;2-w

Cited by 91 publications

(40 citation statements)

References 23 publications

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“…The larger slop in the calibration curve of the lower linear rang may be due to the higher quenching efficiency of 1:1 type of PPEASO3-Cu(II) non-emissive ground state complex formed primarily than other 1:n type complexes formed later in the higher concentration range. Table 2 shows a comparison of figures of merits of some reported optical methods for Cu 2+ determination [12,25,[37][38][39][40][41][42][43][44]. Our method displays comparable high sensitivity and wide linear range, in addition to the advantage of low spectral background interference endowed by NIR emission and long wavelength excitation.…”

Section: Analytical Parametersmentioning

confidence: 99%

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Highly sensitive fluorescent sensor for copper (II) based on amplified fluorescence quenching of a water-soluble NIR emitting conjugated polymer

et al. 2012

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We have synthesized the near-infrared watersoluble conjugated polymer poly[2,5-di(propyloxysulfonate)-1,4-phenylene-ethynylene-9,10-anthrylene (referred to as PPEASO3). Its fluorescence (at wavelengths between 650 and 800 nm following photoexcitation at 550 nm) is efficiently quenched by Cu(II) ions, while other physiologically relevant metal ions do not cause significant quenching at the same concentrations. Under optimum conditions, fluorescence intensity is inversely proportional to the concentration of Cu (II). The calibration curve displays two linear regions over the range of 0-3.2×10 −7 mol L −1 and 3.2×10 −7 mol L −1 to 1.0× 10 −4 mol L −1 of Cu(II), respectively. The long-wavelength excitation and emission can substantially reduce interferences by the autofluorescence and light scattering of biological matter under UV excitation. The method was successfully applied to the determination of Cu(II) in synthetic and tea samples.

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Section: Analytical Parametersmentioning

confidence: 99%

“…From the data given in the Table 3 we can see that the results were satisfactory, suggesting that the method was reliable and applicable. Bioluminescence immobilized engineered microorganisms (AE1239) 0-25000 1000 [41] luminescence Dual lifetime referenced optical sensor membrane 1000-1000000 - [42] Fluorescence quenching…”

Section: Analytical Applicationsmentioning

confidence: 99%

Highly sensitive fluorescent sensor for copper (II) based on amplified fluorescence quenching of a water-soluble NIR emitting conjugated polymer

et al. 2012

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“…The limit of detection (C lim ) was given by the equation, C lim =3δ/k, where δ was the standard deviation of blank determinations (n=10) and k was the slope of calibration graph. The detection limit was found to be 4.3×10 −8 mol L -1 , which was lower than previously reported (Table 2) [16,[36][37][38][39][40].…”

Section: Analytical Parameters and Sample Determinationmentioning

confidence: 99%

Calcein-functionalized Fe3O4@SiO2 nanoparticles as a reusable fluorescent nanoprobe for copper(II) ion

Liu

Zang

et al. 2014

Microchim Acta

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Calcein was first covalently grafted onto the surface of magnetic silica nanoparticles to obtain a novel fluorescent nanoprobe for sensitive and selective determination of Cu(II) ion. The nanoparticles were synthesized in a two-step reaction under mild conditions and characterized using small-angle Xray scattering, Fourier transform infrared spectroscopy, transmission electron microscopy, and thermogravimetric analysis. The nanoprobe also displays good selectivity over other metal ions and is highly stable under conditions of high ionic strength. The response to Cu(II) ranges from 80 nM to 1.25 μM. The detection limit (LOD) is 43 nM which is lower than previously reported LODs. The probe can be regenerated by adding the complexing agent EDTA and also can be separated from a sample mixture by applying a magnetic field. We conclude that this method represents a new and convenient approach to the development of magnetic and reusable optical probes.

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“…Thus, efforts have been devoted to designing suitable and environmentally-friendly sensors for Cu 2+ detection [10]. Unlike above techniques, fluorescence methods offer a promising approach for simple, low-costly, and rapid tracking of metal ions in biological, toxicological, and environmental samples [15][16][17][18][19][20][21][22][23][24][25]. Several organic dye molecules have been demonstrated to be selective for the detection of Cu 2+ ions based on fluorescence methods [15,16].…”

Section: Introductionmentioning

confidence: 99%

Copper nanoclusters coated with bovine serum albumin as a regenerable fluorescent probe for copper(II) ion

et al. 2014

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We show that copper nanoclusters coated with bovine serum albumin are viable probes for fluorometric determination of Cu(II) ion. The effect is attributed to the paramagnetic nature of Cu(II) bound to BSA. The ions Hg(II) and Fe(III) also give a strong effect but can be discerned by addition of EDTA. The probe can be regenerated by adding an excess of EDTA. The method is capable of detecting Cu(II) in the 0.02-34 μM, and the limit of detection is 1nM. The method was applied to the determination of Cu(II) in spiked water samples. It is rapid, simple, and fairly sensitive. The method may also be applied to detect Hg(II) and Fe(III) if Cu(II) is absent.

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Engineered Bacteria Based Biosensors for Monitoring Bioavailable Heavy Metals

Cited by 91 publications

References 23 publications

Highly sensitive fluorescent sensor for copper (II) based on amplified fluorescence quenching of a water-soluble NIR emitting conjugated polymer

Highly sensitive fluorescent sensor for copper (II) based on amplified fluorescence quenching of a water-soluble NIR emitting conjugated polymer

Calcein-functionalized Fe3O4@SiO2 nanoparticles as a reusable fluorescent nanoprobe for copper(II) ion

Copper nanoclusters coated with bovine serum albumin as a regenerable fluorescent probe for copper(II) ion

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