Enemas with n-Acetylcysteine Can Reduce the Level of Oxidative Damage in Cells of the Colonic Mucosa Diverted from the Faecal Stream

Almeida, Marcos Gonçalves de; Silva, Camila Moraes Gonçalves da; Ribeiro, Marcelo Lima; Cunha, Fernando Lorenzetti da; Rodrigues, Murilo Rocha; Sato, Daniela Tiemi; Pereira, José Aires

doi:10.1007/s10620-013-2768-9

Cited by 21 publications

(32 citation statements)

References 38 publications

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“…ROS are produced in excess by colonic mucosa without intestinal transit. The increased production of superoxide radicals, hydroxyl and hypochlorous acid, which cause oxidative stress, can damage the various defense systems of the colonic mucosa 39 . The damage to these defense systems enables the migration of antigens and bacteria in the intestinal lumen to the intima of sterile layers of the colon wall, thus triggering the inflammatory process found in DC 5,6,9 .…”

Section: (5) 2015 -335mentioning

confidence: 99%

Tissue content of sulfomucins and sialomucins in the colonic mucosa, without fecal stream, undergoing daily intervention with sucralfate

et al. 2015

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PURPOSE:To measure the content of acidic mucin, sialomucin, and sulfomucins in the colonic mucosa without fecal stream submit to intervention with sucralfate (SCF). METHODS:Thirty-six rats were submitted to a right colostomy and a distal mucous fistula and divided into two groups according to sacrifice to be performed two or four weeks. Each group was divided into three subgroups according daily application of enemas containing saline, SCF at 1.0 g/kg/day or 2.0 g/kg/day. Colitis was diagnosed by histological analysis. Acid mucins were determined with the Alcian-Blue and sulfomucin and sialomucin by high iron diamine-alcian blue (HID-AB) techniques. The mucins were quantified by computer-assisted image analysis. Mann-Whitney and ANOVA tests were used to analyze the results establishing the level of significance of 5% for both (p<0.05).RESULTS: SCF enemas decreased the inflammation score and was related to the concentration used and time of the intervention. SCF at both concentrations increased the content of acid mucin, which was related to the concentration used and to the improvement in the inflammatory score. There was an increase in the content of sulfomucins and sialomucins in SCF groups. SCF increased sulfomucins from 2 weeks of intervention, which was not related to the dose or time of application. The increase in sialomucin content was related to the time and dose used in the intervention. CONCLUSION:Sucralfate increased the content of acidic mucins, primarily at the expense of sialomucin, which was affected by the dose and time of intervention.

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Section: (5) 2015 -335mentioning

confidence: 99%

Tissue content of sulfomucins and sialomucins in the colonic mucosa, without fecal stream, undergoing daily intervention with sucralfate

et al. 2015

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“…19 MDA is a secondary product of lipid oxidation and is considered a potential candidate for being a general biomarker of oxidative stress. As to the quantification of tissue levels of MDA, 1 g of each fragment was placed in 5 mL of phosphate buffer and homogenized by vortex and ultrasound sonication for 30 s, alternately, repeating the process for three times.…”

Section: Determination Of Malondialdehyde (Mda) Levelsmentioning

confidence: 99%

Evaluation of the anti-inflammatory and antioxidant effects of the sucralfate in diversion colitis

Rodrigues

Sato

Silva

et al. 2015

Journal of Coloproctology

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“…Studies have shown that the application of enemas containing substances with antioxidant activity in colonic segments devoid from the fecal stream can protect and preserve the colonic epithelial barrier integrity 12,13,32 . zThese substances can reduce the epithelial damage, improve the inflammation and increase the mucous layer that recovers the colonic mucosa devoid of the fecal stream [1][2][3] .…”

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Claudin-3 and occludin content in the glands of colonic mucosa devoid from fecal stream submitted to topical intervention with oil extract of Curcuma longa

et al. 2017

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1 8-Experimental SurgeryActa Cir Bras. 2017;32(1):65-73 AbstractPurpose: To evaluate the inflammatory intensity and measure the tissue content of the proteins claudin-3 and occludin in the colonic mucosa without fecal stream submit to intervention with curcumin. Methods: Thirty-six rats were submitted to a proximal colostomy and a distal mucous fistula and divided into two groups according to sacrifice to be performed two or four weeks. Each group was divided into three subgroups according daily application of enemas containing saline, curcumin at 50 mg/kg/day or 200 mg/kg/day. Colitis was diagnosed by histological analysis. Claudin-3 and occludin were determined by immunohistochemistry. The tissue content of claudin-3 and occludin were quantified by computer-assisted image analysis. MannWhitney, Student t and ANOVA tests were used to analyze the results establishing the level of significance of 5% for both (p<0.05). Results: Curcumin at both concentrations reduces the inflammation and preserves the tissue content of the proteins claudin-3 and occludin, which was related to the concentration used and to the time of the intervention. Conclusion:The application of enemas with curcumin reduces inflammation and preserves the tissue content of the proteins claudin-3 and occludin in the colonic mucosa devoid from the fecal stream.

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Enemas with n-Acetylcysteine Can Reduce the Level of Oxidative Damage in Cells of the Colonic Mucosa Diverted from the Faecal Stream

Abstract: Intrarectal application of NAC reduces the inflammation as well as DNA oxidative damage and could be beneficial as a complementary agent in the treatment of DC.

Cited by 21 publications

References 38 publications

Tissue content of sulfomucins and sialomucins in the colonic mucosa, without fecal stream, undergoing daily intervention with sucralfate

Tissue content of sulfomucins and sialomucins in the colonic mucosa, without fecal stream, undergoing daily intervention with sucralfate

Evaluation of the anti-inflammatory and antioxidant effects of the sucralfate in diversion colitis

Claudin-3 and occludin content in the glands of colonic mucosa devoid from fecal stream submitted to topical intervention with oil extract of Curcuma longa

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