Evaluation of the anti-inflammatory and antioxidant effects of the sucralfate in diversion colitis

Rodrigues, Murilo Rocha; Sato, Daniela Tiemi; Silva, Camila Morais Gonçalves da; Kanno, Danilo Toshio; Mendonça, Roberta Lais dos Santos; Pereira, José Aires

doi:10.1016/j.jcol.2015.02.007

Cited by 12 publications

(17 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Recently, experimental studies have shown that the application of enemas with SCF in addition to forming a protective film, firmly adhered to the colonic mucosa, reduced the intensity of the inflammatory process in the mucosa devoid of fecal transit 27 . The improvement of the inflammatory process was shown to be related to the anti-inflammatory, antioxidant and stimulant action of the mucus production that SCF has 3 , 5 , 23 . The SCF application also reduced tissue RLO levels by increasing mucosal layer thickness and tissue content of the mucin molecule’s glucose fractions in the colonic glands.…”

Section: Discussionmentioning

confidence: 99%

“…Study has shown that SCF stimulates mucus production in goblet cells by increasing the production of prostaglandin E2 16 , 30 . It has also been demonstrated that the antioxidant activity of SCF, by removing RLO produced in excess by intestinal mucosa cells devoid of intestinal transit, preserves the integrity of the different defense systems of the colonic mucosa, particularly the mucus layer 20 , 23 . An experimental study reported that the antioxidant activity of SCF reduces the levels of phospholipid oxidation present in the membranes of cells of the colonic mucosa, decreasing apoptosis and, consequently, preserving the integrity of the epithelial barrier 23 .…”

Section: Discussionmentioning

confidence: 99%

“…It has also been demonstrated that the antioxidant activity of SCF, by removing RLO produced in excess by intestinal mucosa cells devoid of intestinal transit, preserves the integrity of the different defense systems of the colonic mucosa, particularly the mucus layer 20 , 23 . An experimental study reported that the antioxidant activity of SCF reduces the levels of phospholipid oxidation present in the membranes of cells of the colonic mucosa, decreasing apoptosis and, consequently, preserving the integrity of the epithelial barrier 23 . It is possible that the SCF also increases the transcription of the MUC-2 gene related to the production of the homonymous protein in the colon epithelium without fecal transit.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

[Retracted Article]: Evaluation of Enemas Containing Sucralfate in Tissue Content of Muc-2 Protein in Experimental Model of Diversion Colitis

Fernandez

Pereira

Campos

et al. 2018

ABCD, arq. bras. cir. dig.

Self Cite

View full text Add to dashboard Cite

Background: The effects of topical application of sucralfate (SCF) on the tissue content of MUC-2 protein have not yet been evaluated in experimental models of diversion colitis. Aim: To measure the tissue content of MUC-2 protein in the colonic mucosa diverted from fecal stream submitted to the SCF intervention. Methods: Thirty-six rats underwent derivation of intestinal transit through proximal colostomy and distal mucous fistula. The animals were divided into three groups which were submitted application of enemas with saline, SCF 1 g/kg/day and SCF 2 g/kg/day. Each group was divided into two subgroups, according to euthanasia was done after two or four weeks. The colitis diagnosis was established by histopathological study and the inflammatory intensity was evaluated by previously validated scale. The MUC-2 protein was identified by immunohistochemistry and the tissue content was measured computerized morphometry). Results: The application of enemas with SCF in the concentration of 2 g/kg/day reduced inflammatory score of the segments that were diverted from fecal stream. The content of MUC-2 in diverted colon of the animals submitted to the intervention with SCF, independently of intervention period and the used concentration, was significantly greater than animals submitted to the application of enemas containing saline (p< 0.01). The content of MUC-2 after the intervention with SCF in the concentration of 2 g/kg/day was significantly higher when compared to the animals submitted to the application containing SCF at concentration of 1.0 g/kg/day (p<0.01). The tissue content of MUC-2 reached the highest values after intervention with SCF in the concentration of 2 g/kg/day for four weeks (p<0.01). Conclusion: The preventive application of enemas containing SCF reduces the inflammatory score and avoids the reduction of tissue content of MUC-2, suggesting that the substance is a valid therapeutic strategy to preserve the mucus layer that covers the intestinal epithelium.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

[Retracted Article]: Evaluation of Enemas Containing Sucralfate in Tissue Content of Muc-2 Protein in Experimental Model of Diversion Colitis

Fernandez

Pereira

Campos

et al. 2018

ABCD, arq. bras. cir. dig.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Similar values of malondialdehyde (MDA) between treatments indicate that S pseudoquina extract does not inhibit tissue oxidation. MDA is a byproduct of lipid peroxidation, derived from the breakdown of polyunsaturated fatty acids (linoleic, arachidonic and docosahexoic acid), considered the general biomarker of lipid oxidative damage in plasma [ 63 ] and the general potential biomarker of oxidative stress [ 64 ]. However, MDA is a very unstable molecule andthus the results obtained by the marking of this product are not completely reliable.…”

Section: Discussionmentioning

confidence: 99%

Strychnos pseudoquina modulates the morphological reorganization of the scar tissue of second intention cutaneous wounds in rats

et al. 2018

View full text Add to dashboard Cite

Natural substances are used in folk medicines to treat injuries. Strychnos pseudoquina has scarring, antipyretic, and antimalarial actions. The present study aimed to analyze the effect of S. pseudoquina on cutaneous wound healing in rats. The S. pseudoquina extract was submitted to phytochemical prospection. The levels of flavonoids and total phenolic compounds in the extract were 50.7 mg/g and 2.59 mg/g, respectively. Thirty Wistar rats were individualized in cages with food and water ad libitum (registration no. 730/2014). After anesthesia, three circular wounds (12mm diameter) were made in the animals, which were randomly separated into five treatments: Sal, saline; VO, ointment vehicles (lanolin and Vaseline); SS, positive control (silver sulfadiazine 1%); LE 5, freeze-dried extract 5%; and LE 10, lyophilized extract 10%. The animals were treated with the ointment daily for 21 days. Every seven days, the area and the rate of wound contraction were evaluated. Tissue samples were removed for histopathological analysis of the number of mast cells, elastic and collagen fibers, and biochemical analyses, quantification of malondialdehyde (MDA), carbonylated proteins (PCN), superoxide dismutase (SOD), catalase (CAT), transforming growth factor β (TGF-β), Interleukin 10 (IL-10) and tumor necrosis factor (TNF). The number of mast cells, collagen and elastic fibers in the rat wounds were higher in the treatments with the plant. The extract also stimulated the activity of antioxidant enzymes, particularly SOD, presenting high levels, and maintained low levels of PCN. The TGF-β and IL-10 concentration was higher in the LE5 and LE10 treatment of the extract than in the Sal, OV and SS treatments on day 7. The ointment based on S. pseudoquina closed the wound faster and accelerated wound healing in animals.

show abstract

“…The immunohistochemical technique for the investigation of tissue expression of MPO was performed using a previously described methodology 22 . Briefly, all blocks were sectioned in 5µ thick sections obtained from colonic segments with or without fecal stream of the animals treated with saline or both concentration of infliximab.…”

Section: Methodsmentioning

confidence: 99%

Effects of anti-TNF-α in experimental diversion colitis

et al. 2019

Self Cite

View full text Add to dashboard Cite

Purpose:To evaluate the effects of infliximab on the inflammation of the colonic mucosa devoid from fecal stream.Methods:Twenty-four rats were submitted to a Hartmann's procedure. They remained for 12 weeks with the fecal derivation to development of diversion colitis on excluded colorectal stump. After this period, they were divided into 3 groups: one group received intervention with saline (2.0 mL / week), other group infliximab at doses of 5 mg/kg/week and the other 10 mg/kg/week for five consecutively weeks. Concluded the intervention period, the animals were euthanized to remove colon segments with and without fecal stream. Colitis was diagnosed by histological analysis and the degree of inflammation by validated score. The neutrophilic infiltrate was evaluated by tissue expression of myeloperoxidase identified by immunohistochemical. The tissue content of myeloperoxidase was measured by computer-assisted image analysis.Results:The inflammatory score was high in colonic segments without fecal stream. The intervention with infliximab reduced the inflammatory score in excluded colonic segments. The content of myeloperoxidase was reduced in colonic segments of animals treated with infliximab mainly in high concentrations.Conclusion:Intervention with infliximab reduced the inflammation and the neutrophil infiltrate in colonic segments devoid of the fecal stream.

show abstract

Evaluation of the anti-inflammatory and antioxidant effects of the sucralfate in diversion colitis

Abstract: Please cite this article in press as: Martinez CAR, et al. Evaluation of the anti-inflammatory and antioxidant effects of the sucralfate in diversion colitis. J Coloproctol (Rio J). 2015. http://dx.

Cited by 12 publications

References 37 publications

[Retracted Article]: Evaluation of Enemas Containing Sucralfate in Tissue Content of Muc-2 Protein in Experimental Model of Diversion Colitis

[Retracted Article]: Evaluation of Enemas Containing Sucralfate in Tissue Content of Muc-2 Protein in Experimental Model of Diversion Colitis

Strychnos pseudoquina modulates the morphological reorganization of the scar tissue of second intention cutaneous wounds in rats

Effects of anti-TNF-α in experimental diversion colitis

Contact Info

Product

Resources

About