“…Previously described protocols were used to perform the immunohistochemistry for calbindin, TNF-α, and active caspase-8. 8,9,18,[23][24][25][26][27] Briefly, the 4-µm-thick sections were processed via immunohistochemistry using an antibody to TNF-α (ab6671, Abcam, Cambridge, MA, USA) and an antibody to active caspase-8 (sc-5263, Santa Cruz Biotechnology, Santa Cruz, CA, USA). Subsequent to their deparaffinization and rehydration, the sections were processed for antigen retrieval.…”